公开(公告)号：US20220091109A1

公开(公告)日：2022-03-24

申请号：US17481175

申请日：2021-09-21

Applicant: Applied Materials, Inc.

Inventor： Zhen Li ,  Marc Glazer ,  Yun-Ching Chang

IPC: G01N33/533 ,  G01N21/64

Abstract: A readout probe for use in fluorescent in-situ hybridization imaging has a targeting portion to bind to a first hybridization sequence in an encoding probe that targets an analyte in a sample, a first fluorophore to emit light at a wavelength range, and a first cleaving region between the first fluorophore and the targeting portion.

公开(公告)号：US20220092288A1

公开(公告)日：2022-03-24

申请号：US17481197

申请日：2021-09-21

Applicant: Applied Materials, Inc.

Inventor： Zhen Li ,  Marc Glazer ,  Yun-Ching Chang

IPC: G06K9/00 ,  C12Q1/6841 ,  B01L3/00 ,  G01N21/64 ,  G02B21/16 ,  G02B21/36

Abstract: A computer program product includes instructions to cause one or more computers to obtain first and second images in the same color channel in the same hybridization step from a fluorescent in-situ hybridization imaging system, and to register the first image and the second image. One or more pixels having a first intensity exceeding a first threshold in the first image and having a second intensity exceeding a second threshold in the second image are identified and classified as activated in a first readout call, and one or more pixels having the first intensity exceeding the first threshold in the first image and the second intensity below the second threshold in the second image are identified and classified as activated in a second readout call.

公开(公告)号：US20220090182A1

公开(公告)日：2022-03-24

申请号：US17481161

申请日：2021-09-21

Applicant: Applied Materials, Inc.

Inventor： Zhen Li ,  Marc Glazer ,  Yun-Ching Chang

IPC: C12Q1/6841 ,  C12Q1/6876 ,  C12Q1/6825 ,  G01N21/64

Abstract: A method of fluorescent in-situ hybridization imaging includes exposing a sample to a first plurality of first readout probes and a second plurality of second readout probes, obtaining a first image of the sample with first readout probes and the second readout probes bound to a first analyte and a second analyte, respectively, so as to emit light at a first wavelength range, treating the sample so as to modify the second readout probes, and obtaining a second image of the sample with the first readout probes bound to the first analyte so as to emit light at the first wavelength range and the second analyte substantially not emitting light at the first wavelength range.