摘要:
Methods and apparatus for imaging a sample using a microwell array are provided. The methods and apparatus allow side view imaging of a sample to acquire fluorescence or bright field images.
摘要:
An optical apparatus includes a system of optical components capable of operating in a scanning laser ophthalmoscope (SLO) mode and an optical coherence tomography (OCT) mode. The system of optical components includes a first optical module for the SLO mode, a second optical module for the OCT mode, and a first scanning device. The first optical module for the SLO mode includes a first source adapted to provide a first imaging beam for the SLO mode and a first detection device configured to receive a first signal associated with a first image of a retina of an eye. The second optical module for the OCT mode includes a second source adapted to provide a second imaging beam for the OCT mode and a second detection device configured to receive a second signal associated with a second image of the retina. The first scanning device is configured to move the first imaging beam along the retina in the slow axis of the SLO mode to acquire the first image and (ii) to move the second imaging beam along the retina in the fast axis of the OCT mode to acquire the second image.
摘要:
Real time, high-speed image stabilization with a retinal tracking scanning laser ophthalmoscope (TSLO) enables new approaches to established diagnostics. Large frequency range (DC to 19 kHz), wide-field (40-deg) stabilized Doppler flowmetry imaging is described for human subjects. The fundus imaging method is a quasi-confocal line-scanning laser ophthalmoscope (LSLO). The retinal tracking system uses a confocal reflectometer with a closed loop optical servo system to lock onto features in the ocular fundus and automatically re-lock after blinks. By performing a slow scan with the laser line imager, frequency-resolved retinal perfusion and vascular flow images can be obtained free of eye motion artifacts.
摘要:
Apparatus and methods for tracking a feature on a target surface and continually providing analog corrections to tracking mirrors in real time by utilizing a low-power incoherent tracking beam to detect the movements of a reference feature on the target and confocal reflectometry to monitor the reflection from the tracking beam's current position are described. The apparatus includes a dithering device for dithering the tracking beam in a first and a second direction with an oscillatory motion, a tracking device for controlling the position of a therapeutic beam relative to a target and for controlling the position of the tracking beam relative to a reference feature, a reflectometer for providing an output signal with a phase corresponding to a phase of the reflected tracking beam, and a signal processor for comparing the phase of the reflectometer output signal to the phases of the oscillatory motion and for controlling the tracking device so that the therapeutic beam to tracks relative to the reference feature.
摘要:
Apparatus and methods for tracking a feature on a target surface and continually providing analog corrections to tracking mirrors in real time by utilizing a low-power incoherent tracking beam to detect the movements of a reference feature on the target and confocal reflectometry to monitor the reflection from the tracking beam's current position are described. The apparatus includes a dithering device for dithering the tracking beam in a first and a second direction with an oscillatory motion, a tracking device for controlling the position of a therapeutic beam relative to a target and for controlling the position of the tracking beam relative to a reference feature, a reflectometer for providing an output signal with a phase corresponding to a phase of the reflected tracking beam, and a signal processor for comparing the phase of the reflectometer output signal to the phases of the oscillatory motion and for controlling the tracking device so that the therapeutic beam to tracks relative to the reference feature.
摘要:
A system provides an optical image of an object. A first module tracks a reference feature of the object. A second module includes a source for an imaging beam, a scanning device to move the imaging beam along a portion of the object and a detection device receives a signal associated with an image of the portion of the object. The first module controls the position of the imaging beam relative to the reference feature to correct for the motion of the object. A third module detects a distortion of the object and compensates for the distortion.
摘要:
A system provides an optical image of an object. A first module tracks a reference feature of the object. A second module includes a source for an imaging beam, a scanning device to move the imaging beam along a portion of the object and a detection device receives a signal associated with an image of the portion of the object. The first module controls the position of the imaging beam relative to the reference feature to correct for the motion of the object. A third module detects a distortion of the object and compensates for the distortion.
摘要:
A system provides an optical image of an object. A first module tracks a reference feature of the object. A second module includes a source for an imaging beam, a scanning device to move the imaging beam along a portion of the object and a detection device receives a signal associated with an image of the portion of the object. The first module controls the position of the imaging beam relative to the reference feature to correct for the motion of the object. A third module detects a distortion of the object and compensates for the distortion.
摘要:
An apparatus for imaging an eye includes a housing and a system of optical components disposed in the housing. The apparatus is capable of operating in a line scanning laser ophthalmoscope (LSLO) mode and an optical coherence tomography (OCT) mode. The system of optical components can include a first source to provide a first beam of light for the OCT mode and a second source to provide a second beam of light for the LSLO mode. In the OCT mode, a first optic is used that (i) scans, using a first surface of the first optic, the first beam of light along a retina of an eye in a first dimension, and (ii) descans, using the first surface, a first light returning from the eye in the first dimension to a detection system in the OCT mode. In the LSLO mode, the first optic is used where the second beam of light passes through a second surface of the first optic.
摘要:
A first optical module scans a portion of an eye with a line of light, descans reflected light from the scanned portion of the eye and confocally provides output light in a line focus configuration. A detection device detects the output light and images the portion of the eye. A second optical module detects an optical distortion and corrects the optical distortion in the line of light scanned on the portion of the eye.