公开(公告)号：US20230323287A1

公开(公告)日：2023-10-12

申请号：US18034620

申请日：2021-11-01

Applicant: AMGEN INC.

Inventor： Kristine Marie DARIS ,  Fides Dwinan LAY

IPC: C12N5/073 ,  C07K14/72 ,  C12M1/00

CPC classification number: C12N5/0604 ,  C07K14/72 ,  C12M29/10 ,  C12N2510/02 ,  C12N2501/105

Abstract: Methods of mammalian cell culture for expressing a recombinant protein of interest are provided. In various embodiments the methods relate to the mammalian cells expressing an IGF1R mutant that is constitutively active.

公开(公告)号：US20230082811A1

公开(公告)日：2023-03-16

申请号：US17930810

申请日：2022-09-09

Applicant: Amgen Inc.

Inventor： Kristine Marie DARIS ,  Huong Thi Ngoc LE ,  Eric GISLASON ,  Trent Phillip MUNRO

IPC: C12P21/02 ,  C12N5/00 ,  C12N15/90 ,  C07K16/00

Abstract: Methods producing a recombinant protein of interest in a mammalian cell culture in media lacking IGF-1 are provided. Methods for producing mammalian cells capable of growing in media lacking IGF-1 are also provided.