公开(公告)号：US20160377614A1

公开(公告)日：2016-12-29

申请号：US15057822

申请日：2016-03-01

Applicant: Alere Scarborough, Inc.

Inventor： Norman J. Moore ,  Mary K. Konstantin ,  Vladimir A. Koulchin ,  Elena V. Molokova

IPC: G01N33/569 ,  G01N21/78

CPC classification number: G01N33/56944 ,  C07K16/1275 ,  G01N21/78 ,  G01N2021/752 ,  G01N2021/757 ,  G01N2021/7759 ,  G01N2333/3156 ,  G01N2400/10 ,  G01N2469/10

Abstract: Disclosed is a cell wall C-polysaccharide antigen of Streptococcus pneumoniae which contains not more than 10% by weight of protein, and preferably less which has been purified with 0.1N NaOH prior to deproteinizing. Also disclosed are polyvalent antibodies raised against Streptococcus pneumoniae which have been affinity purified by passing them over a chromatographic affinity matrix to which is coupled the purified and at least partially deproteinized antigen to render them antigen-specific.

Abstract translation: 公开了肺炎链球菌的细胞壁C-多糖抗原，其含有不超过10重量％的蛋白质，并且优选较少，其在脱蛋白之前用0.1N NaOH纯化。 还公开了针对肺炎链球菌产生的多价抗体，其通过将它们通过将纯化的和至少部分去蛋白质的抗原偶联以使其成为抗原特异性的色谱亲和基质将其亲和纯化。

公开(公告)号：US20160047807A1

公开(公告)日：2016-02-18

申请号：US14828823

申请日：2015-08-18

Applicant: Alere Scarborough, Inc.

Inventor： Vladimir A. Koulchin ,  Norman J. Moore ,  Elena V. Molokova

IPC: G01N33/569 ,  G01N21/78

CPC classification number: G01N33/56911 ,  C07K16/126 ,  G01N21/78 ,  G01N33/53 ,  G01N2021/752 ,  G01N2021/757 ,  G01N2021/7759 ,  G01N2400/10 ,  G01N2469/10

Abstract: The present invention involves extracting from Legionella bacteria, particularly L. pneumophila bacteria, an essentially protein-free O-polysaccharide or carbohydrate antigen, coupling this antigen to an activated chromatographic column through a protein space molecule which is first conjugated to the antigen, utilizing the column thus prepared for the affinity purification of raw polyvalent antibodies to the same Legionella bacterium from which the O-polysaccharide or carbohydrate antigen was separated—thereby obtaining antigen-specific antibodies which are useful for the rapid detection of the corresponding Legionella bacterium or its antibody in human bodily fluids such as urine, sputum, blood and the like or in environmental samples suspected of harboring theLegionella bacterium.

Abstract translation: 本发明涉及从军团菌细菌，特别是嗜肺链球菌细菌，一种基本上无蛋白的O-多糖或碳水化合物抗原提取，该抗原通过首先与抗原缀合的蛋白质空间分子将该抗原与活化的色谱柱偶联， 将这样制备的原料多价抗体亲和纯化到与其分离O-多糖或碳水化合物抗原的相同军团杆菌细菌中，从而获得可用于快速检测相应的军团菌或其抗体的抗原特异性抗体 人体液体如尿液，痰液，血液等，或涉嫌怀疑含有白血病细菌的环境样品。

公开(公告)号：US09989529B2

公开(公告)日：2018-06-05

申请号：US14828823

申请日：2015-08-18

Applicant: Alere Scarborough, Inc.

Inventor： Vladimir A. Koulchin ,  Norman J. Moore ,  Elena V. Molokova

IPC: C12Q1/68 ,  G01N33/569 ,  C07K16/12 ,  G01N33/53 ,  G01N21/78 ,  G01N21/75 ,  G01N21/77

CPC classification number: G01N33/56911 ,  C07K16/126 ,  G01N21/78 ,  G01N33/53 ,  G01N2021/752 ,  G01N2021/757 ,  G01N2021/7759 ,  G01N2400/10 ,  G01N2469/10

Abstract: The present invention involves extracting from Legionella bacteria, particularly L. pneumophila bacteria, an essentially protein-free O-polysaccharide or carbohydrate antigen, coupling this antigen to an activated chromatographic column through a protein space molecule which is first conjugated to the antigen, utilizing the column thus prepared for the affinity purification of raw polyvalent antibodies to the same Legionella bacterium from which the O-polysaccharide or carbohydrate antigen was separated—thereby obtaining antigen-specific antibodies which are useful for the rapid detection of the corresponding Legionella bacterium or its antibody in human bodily fluids such as urine, sputum, blood and the like or in environmental samples suspected of harboring theLegionella bacterium.

公开(公告)号：US09921220B2

公开(公告)日：2018-03-20

申请号：US15057822

申请日：2016-03-01

Applicant: Alere Scarborough, Inc.

Inventor： Norman J. Moore ,  Mary K. Konstantin ,  Vladimir A. Koulchin ,  Elena V. Molokova

IPC: G01N33/569 ,  C07K16/12 ,  G01N21/78 ,  G01N21/75 ,  G01N21/77

CPC classification number: G01N33/56944 ,  C07K16/1275 ,  G01N21/78 ,  G01N2021/752 ,  G01N2021/757 ,  G01N2021/7759 ,  G01N2333/3156 ,  G01N2400/10 ,  G01N2469/10

Abstract: Disclosed is a cell wall C-polysaccharide antigen of Streptococcus pneumoniae which contains not more than 10% by weight of protein, and preferably less which has been purified with 0.1N Na OH prior to deproteinizing. Also disclosed are polyvalent antibodies raised against Streptococcus pneumoniae which have been affinity purified by passing them over a chromatographic affinity matrix to which is coupled the purified and at least partially deproteinized antigen to render them antigen-specific.

公开(公告)号：US09310369B2

公开(公告)日：2016-04-12

申请号：US14628827

申请日：2015-02-23

Applicant: Alere Scarborough, Inc.

Inventor： Norman J. Moore ,  Mary K. Konstantin ,  Vladimir A. Koulchin ,  Elena V. Molokova

IPC: G01N33/558 ,  G01N33/569 ,  C07K16/12

CPC classification number: G01N33/56944 ,  C07K16/1275 ,  G01N21/78 ,  G01N2021/752 ,  G01N2021/757 ,  G01N2021/7759 ,  G01N2333/3156 ,  G01N2400/10 ,  G01N2469/10

Abstract: Disclosed is a cell wall C-polysaccharide antigen of Streptococcus pneumoniae which contains not more than 10% by weight of protein, and preferably less which has been purified with 0.1N Na OH prior to deproteinizing. Also disclosed are polyvalent antibodies raised against Streptococcus pneumoniae which have been affinity purified by passing them over a chromatographic affinity matrix to which is coupled the purified and at least partially deproteinized antigen to render them antigen-specific.

Abstract translation: 公开了一种细胞壁C型多糖抗原，其含有不超过10重量％的蛋白质，优选较少的是在脱蛋白之前用0.1N Na OH纯化的。 还公开了针对肺炎链球菌产生的多价抗体，其通过将它们通过将纯化的和至少部分去蛋白质的抗原偶联以使其成为抗原特异性的色谱亲和基质将其亲和纯化。

公开(公告)号：US20150219648A1

公开(公告)日：2015-08-06

申请号：US14628827

申请日：2015-02-23

Applicant: Alere Scarborough, Inc.

Inventor： Norman J. Moore ,  Mary K. Fent ,  Vladimir A. Koulchin ,  Elena V. Molokova

IPC: G01N33/569

CPC classification number: G01N33/56944 ,  C07K16/1275 ,  G01N21/78 ,  G01N2021/752 ,  G01N2021/757 ,  G01N2021/7759 ,  G01N2333/3156 ,  G01N2400/10 ,  G01N2469/10

Abstract: Disclosed is a cell wall C-polysaccharide antigen of Streptococcus pneumoniae which contains not more than 10% by weight of protein, and preferably less which has been purified with 0.1N Na OH prior to deproteinizing. Also disclosed are polyvalent antibodies raised against Streptococcus pneumoniae which have been affinity purified by passing them over a chromatographic affinity matrix to which is coupled the purified and at least partially deproteinized antigen to render them antigen-specific.

Abstract translation: 公开了一种细胞壁C型多糖抗原，其含有不超过10重量％的蛋白质，优选较少的是在脱蛋白之前用0.1N Na OH纯化的。 还公开了针对肺炎链球菌产生的多价抗体，其通过将它们通过将纯化的和至少部分去蛋白质的抗原偶联以使其成为抗原特异性的色谱亲和基质将其亲和纯化。