公开(公告)号：US20250035627A1

公开(公告)日：2025-01-30

申请号：US18778278

申请日：2024-07-19

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY ,  Mayo Foundation for Medical Education and Research

Inventor： Shaopeng WANG ,  Nanxi YU ,  Chao CHEN ,  Xinyu ZHOU ,  Januario E. CASTRO ,  Eider F. Moreno Cortes

IPC: G01N33/569 ,  B01L3/00 ,  C12N5/00 ,  G01N33/543 ,  G01N33/68 ,  G06V10/70

Abstract: Provided herein are methods of differentiating cell types in a cell population. The methods include removing at least some non-Chimeric Antigen Receptor (CAR)-T cells from a fluidic sample obtained from a subject without centrifuging the fluidic sample to produce a purified fluidic sample. The fluidic sample comprises CAR-T cells and the non-CAR-T cells. The methods also include capturing cells in the purified fluidic sample on a surface that comprises binding moieties that bind at least to the CAR-T cells to produce a captured cell population. In addition, the methods also include distinguishing the CAR-T cells from the non-CAR-T cells in the captured cell population using a trained machine learning model to produce a captured CAR-T cell population data set. Additional methods as well as related devices and systems are also provided.

公开(公告)号：US20250164509A1

公开(公告)日：2025-05-22

申请号：US18949000

申请日：2024-11-15

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Shaopeng WANG ,  Chao CHEN

IPC: G01N33/74 ,  B01L3/00 ,  G01N33/543

Abstract: Provided herein are methods of quantifying amounts of N-terminal prohormone B-type natriuretic peptide (NT-proBNP) in whole blood samples. The methods include contacting detection antibody-NT-proBNP complexes with a plurality of capture antibodies, or antigen binding portions thereof, that specifically bind to the NT-proBNP molecules of the detection antibody-NT-proBNP complexes to form captured NT-proBNP complexes, and contacting NPs that each comprise a second recognition moiety that binds to the first recognition moiety of the detection antibodies, or antigen binding portions thereof, of the captured NT-proBNP complexes to form captured NP-NT-proBNP complexes. The methods also include taking images of the captured NP-NT-proBNP complexes to produce imaged captured NP-NT-proBNP complexes using a detection mechanism, and quantifying an amount of NT-proBNP in the sample aliquots using the imaged captured NP-NT-proBNP complexes. Additional methods as well as related devices and systems are also provided.