公开(公告)号：US20240083972A1

公开(公告)日：2024-03-14

申请号：US18268639

申请日：2021-12-21

Applicant: AMGEN INC.

Inventor： Eliyahu Kraus ,  Venkatesh Natarajan ,  Ritsdeliz Perez Rodriguez ,  Omar Cruz Nieves

IPC: C07K14/715 ,  C12N5/00 ,  C12N5/071

CPC classification number: C07K14/7151 ,  C12N5/0018 ,  C12N5/0682 ,  A61K38/00 ,  C12N2500/12 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/46 ,  C12N2501/998

Abstract: The disclosure provides a method of producing etanercept from Chinese hamster ovary cells (CHO), the method comprising running an N-1 bioreactor system using a recirculating tangential flow filtration (RTF) or alternating tangential flow filtration (ATF) cell retention device under conditions that maintain a cell aggregate size of at least 20 μm, before running an N production bioreactor.