公开(公告)号：US20200323958A1

公开(公告)日：2020-10-15

申请号：US16763515

申请日：2018-11-05

Applicant: AMGEN INC.

Inventor： Eliyahu Kraus

IPC: A61K38/17 ,  C12M1/34 ,  A61K47/02 ,  A61K47/26

Abstract: The invention pertains to methods of improving the yield and preserving the quality of recombinantly produced proteins and polypeptides in a production bioreactor process by manipulating or controlling the temperature of a bioreactor process that precedes the ultimate production bioreactor process. In particular embodiments the invention provides such methods as well as recombinant proteins and polypeptides produced using such methods, formulations thereof, and methods of treating patients using such proteins, polypeptides, and formulations.

公开(公告)号：US20240083972A1

公开(公告)日：2024-03-14

申请号：US18268639

申请日：2021-12-21

Applicant: AMGEN INC.

Inventor： Eliyahu Kraus ,  Venkatesh Natarajan ,  Ritsdeliz Perez Rodriguez ,  Omar Cruz Nieves

IPC: C07K14/715 ,  C12N5/00 ,  C12N5/071

CPC classification number: C07K14/7151 ,  C12N5/0018 ,  C12N5/0682 ,  A61K38/00 ,  C12N2500/12 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/46 ,  C12N2501/998

Abstract: The disclosure provides a method of producing etanercept from Chinese hamster ovary cells (CHO), the method comprising running an N-1 bioreactor system using a recirculating tangential flow filtration (RTF) or alternating tangential flow filtration (ATF) cell retention device under conditions that maintain a cell aggregate size of at least 20 μm, before running an N production bioreactor.