公开(公告)号：US20240343832A1

公开(公告)日：2024-10-17

申请号：US18644757

申请日：2024-04-24

Applicant: AMGEN INC.

Inventor： JOON HOI HUH ,  RIKI STEVENSON ,  PAVEL BONDARENKO ,  ANDREW NICHOLS ,  DA REN ,  NEERAJ JAGDISH AGRAWAL ,  RICHARD SMITH

IPC: C07K16/40 ,  C07K16/28 ,  G01N11/14

CPC classification number: C07K16/40 ,  C07K16/2869 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/52 ,  C07K2317/55 ,  C07K2317/90 ,  C07K2317/92 ,  C07K2317/94 ,  G01N11/14

Abstract: The present invention concerns a method for preparing antigen binding proteins specific for PCSK9 with reduced viscosity. The method proceeds by replacing residues in high viscosity variable domain subfamilies with residues in correlating low viscosity subfamilies. The method further comprises substituting residues in the Fc domain with residues associated with low viscosity and adding charged residues to the C-terminus of the Fc domain. The present invention further concerns antigen binding proteins produced by this method.

公开(公告)号：US20130144041A1

公开(公告)日：2013-06-06

申请号：US13678376

申请日：2012-11-15

Applicant: AMGEN INC.

Inventor： THOMAS M. DILLON ,  PAVEL BONDARENKO ,  JETTE WYPYCH ,  MARTIN J. ALLEN ,  ALAIN BALLAND ,  MARGARET SPEED RICCI ,  AMY GUO ,  WENYAN SHEN ,  JEONGHOON SUN ,  CHRIS VEZINA

IPC: C07K16/24

CPC classification number: C07K16/245 ,  C07K16/00 ,  C07K16/2863 ,  C07K16/2866 ,  C07K16/2875 ,  C07K2317/53 ,  C07K2317/56

Abstract: The present invention is generally directed to methods of producing an increase in the enrichment and/or recovery of preferred forms of monoclonal antibodies. More particularly, the invention relates to methods for eliminating disulfide heterogeneity in the hinge region of recombinant IgG2 antibody proteins.

Abstract translation: 本发明一般涉及产生增殖和/或回收优选形式的单克隆抗体的方法。 更具体地说，本发明涉及消除重组IgG2抗体蛋白的铰链区中二硫键异质性的方法。

公开(公告)号：US20210371544A1

公开(公告)日：2021-12-02

申请号：US17346156

申请日：2021-06-11

Applicant: AMGEN INC.

Inventor： JOON HOI HUH ,  RIKI STEVENSON ,  PAVEL BONDARENKO ,  ANDREW NICHOLS ,  DA REN ,  NEERAJ JAGDISH AGRAWAL ,  RICHARD SMITH

IPC: C07K16/40 ,  C07K16/28

Abstract: The present invention concerns a method for preparing antigen binding proteins specific for PCSK9 with reduced viscosity. The method proceeds by replacing residues in high viscosity variable domain subfamilies with residues in correlating low viscosity subfamilies. The method further comprises substituting residues in the Fc domain with residues associated with low viscosity and adding charged residues to the C-terminus of the Fc domain. The present invention further concerns antigen binding proteins produced by this method.