公开(公告)号：US20130144041A1

公开(公告)日：2013-06-06

申请号：US13678376

申请日：2012-11-15

Applicant: AMGEN INC.

Inventor： THOMAS M. DILLON ,  PAVEL BONDARENKO ,  JETTE WYPYCH ,  MARTIN J. ALLEN ,  ALAIN BALLAND ,  MARGARET SPEED RICCI ,  AMY GUO ,  WENYAN SHEN ,  JEONGHOON SUN ,  CHRIS VEZINA

IPC: C07K16/24

CPC classification number: C07K16/245 ,  C07K16/00 ,  C07K16/2863 ,  C07K16/2866 ,  C07K16/2875 ,  C07K2317/53 ,  C07K2317/56

Abstract: The present invention is generally directed to methods of producing an increase in the enrichment and/or recovery of preferred forms of monoclonal antibodies. More particularly, the invention relates to methods for eliminating disulfide heterogeneity in the hinge region of recombinant IgG2 antibody proteins.

Abstract translation: 本发明一般涉及产生增殖和/或回收优选形式的单克隆抗体的方法。 更具体地说，本发明涉及消除重组IgG2抗体蛋白的铰链区中二硫键异质性的方法。