公开(公告)号：US20240209115A1

公开(公告)日：2024-06-27

申请号：US18556998

申请日：2022-04-28

Applicant: AMGEN INC. ,  AMGEN RESEARCH (MUNICH) GmbH

Inventor： Anika MENTHE ,  Joseph BASCONI ,  Nitya M. JACOB ,  Russell JAMPOL ,  Huong Thi Ngoc LE ,  Kim LE ,  Jessica PEDROSO ,  Jennitte STEVENS ,  Sreekanth SURAVAJJALA ,  Charilyn TEJAMO ,  Pheng YAM

IPC: C07K16/40 ,  C07K16/28 ,  C07K16/46

CPC classification number: C07K16/40 ,  C07K16/2809 ,  C07K16/468 ,  C07K2317/31 ,  C07K2317/622 ,  C07K2319/02

Abstract: The present invention relates to methods for reducing low molecular weight species of recombinantly-produced proteins. In particular, methods of reducing the formation of low molecular weight species produced by a host cell during the cell culture process through pH control of the production cell culture are disclosed. Also disclosed are methods for reducing or eliminating the generation of alternative splice variants by a host cell during production of a recombinant protein.

公开(公告)号：US20230082811A1

公开(公告)日：2023-03-16

申请号：US17930810

申请日：2022-09-09

Applicant: Amgen Inc.

Inventor： Kristine Marie DARIS ,  Huong Thi Ngoc LE ,  Eric GISLASON ,  Trent Phillip MUNRO

IPC: C12P21/02 ,  C12N5/00 ,  C12N15/90 ,  C07K16/00

Abstract: Methods producing a recombinant protein of interest in a mammalian cell culture in media lacking IGF-1 are provided. Methods for producing mammalian cells capable of growing in media lacking IGF-1 are also provided.

公开(公告)号：US20220064610A1

公开(公告)日：2022-03-03

申请号：US17299000

申请日：2019-12-09

Applicant: AMGEN INC.

Inventor： Neeraj J. AGRAWAL ,  Kristine M. DARIS ,  Jennitte L. STEVENS ,  Huong Thi Ngoc LE ,  Noelia Blanco TALAVAN

IPC: C12N9/12 ,  C12N15/85 ,  C07K14/725 ,  C12N15/90 ,  A61K35/17

Abstract: PiggyBac transposases engineered to increase stability in a cell. The engineered piggyBac transposases are useful for stably transforming cells, cell line development, genome modification, and improving titer of recombinant proteins, among other uses.