公开(公告)号：US10808264B2

公开(公告)日：2020-10-20

申请号：US16032206

申请日：2018-07-11

Applicant: AJINOMOTO CO., INC.

Inventor： Yasuaki Takakura ,  Keiko Danjo ,  Keita Fukui ,  Sayaka Asari ,  Takuto Ono

IPC: C12P7/24

Abstract: A method for producing benzaldehyde is provided. Benzaldehyde is produced from L-phenylalanine or a carbon source by using microorganism(s) having amino acid deaminase, 4-hydroxymandelate synthase, (S)-mandelate dehydrogenase, and benzoylformate decarboxylase.

公开(公告)号：US20140234916A1

公开(公告)日：2014-08-21

申请号：US14230766

申请日：2014-03-31

Applicant: Ajinomoto Co., Inc.

Inventor： Yasuaki Takakura ,  Hiroomi Ogino ,  Masakazu Sugiyama ,  Kenichi Mori ,  Eri Tabuchi ,  Koki Ishikawa ,  Uno Tagami ,  Hidemi Fujii

IPC: C12P13/04

CPC classification number: C12P13/04 ,  C12N9/1096 ,  C12P17/10

Abstract: The present invention provides a methodology for improving a yield of 2R,4R-Monatin. Specifically, the present invention provides a method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-amino acid aminotransferase in the presence of an L-amino acid to form the 2S,4R-Monatin; a method for producing 2R,4R-Monatin or a salt thereof, comprising isomerizing the 2S,4R-Monatin to form the 2R,4R-Monatin; and the like. These production methods may further comprise condensing indole-3-pyruvate and pyruvate to form the 4R-IHOG, and deaminating a tryptophan to form the indole-3-pyruvate.

Abstract translation: 本发明提供了提高2R，4R-莫纳甜产率的方法。 具体地说，本发明提供了制备2S，4R-莫纳甜或其盐的方法，包括在L-氨基酸的存在下将4R-1HOG与L-氨基酸氨基转移酶接触以形成2S，4R-莫纳甜; 一种制备2R，4R-莫纳甜或其盐的方法，包括使2S，4R-莫纳甜异构化形成2R，4R-莫纳甜; 等等。 这些制备方法还可以包括将吲哚-3-丙酮酸和丙酮酸缩合形成4R-1HOG，并使色氨酸脱氨基形成吲哚-3-丙酮酸盐。

公开(公告)号：US20220251611A1

公开(公告)日：2022-08-11

申请号：US17730360

申请日：2022-04-27

Applicant: AJINOMOTO CO., INC.

Inventor： Yasuaki Takakura ,  Takuto Ono ,  Olga Igonina ,  Keiko Koenuma ,  Hiroyuki Nozaki

IPC: C12P7/24

Abstract: A method for producing benzaldehyde is provided. Benzaldehyde is produced by a method including a step of using amino acid deaminase (AAD), 4-hydroxymandelate synthase (HMAS), (S)-mandelate dehydrogenase (SMDH), and benzoylformate decarboxylase (BFDC) over a time period, wherein the producing step is carried out in the presence of catalase during a portion of said time period.

公开(公告)号：US20180312881A1

公开(公告)日：2018-11-01

申请号：US16032206

申请日：2018-07-11

Applicant: AJINOMOTO CO., INC.

Inventor： Yasuaki Takakura ,  Keiko Danjo ,  Keita Fukui ,  Sayaka Asari ,  Takuto Ono

IPC: C12P7/24

CPC classification number: C12P7/24 ,  C12Y101/99031 ,  C12Y104/03002 ,  C12Y113/11046 ,  C12Y401/01007

Abstract: A method for producing benzaldehyde is provided. Benzaldehyde is produced from L-phenylalanine or a carbon source by using microorganism(s) having amino acid deaminase, 4-hydroxymandelate synthase, (S)-mandelate dehydrogenase, and benzoylformate decarboxylase.