公开(公告)号：US20140255924A1

公开(公告)日：2014-09-11

申请号：US13791616

申请日：2013-03-08

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： NICHOLAS M. SAMPAS ,  Kristin Bernick

IPC: C12Q1/68

CPC classification number: C12Q1/682 ,  C12Q1/6818 ,  C12Q2525/161 ,  C12Q2565/101

Abstract: A composition comprising population of labeled oligonucleotides is provided herein. In some embodiments, the probes are of the formula: T1-V-T2, wherein: the nucleotide sequence of the V region varies in said population; the V regions of the different oligonucleotides hybridize to sites that are tiled across a sequence in a target nucleic acid; the T1 and T2 regions do not hybridize with said target nucleic acid; within each oligonucleotide of the population, the T1 and T2 regions are not complementary; and within each oligonucleotide of the population, the T1 region is complementary to the T2 region of at least one of other oligonucleotide of the population. Also provided is a method that comprises hybridizing the population of labeled oligonucleotides with a target nucleic acid to produce a complex.

Abstract translation: 提供包含标记寡核苷酸群的组合物。 在一些实施方案中，探针具有下式：T1-V-T2，其中：V区的核苷酸序列在所述群体中变化; 不同寡核苷酸的V区与靶核酸中的序列平铺的位点杂交; T1和T2区不与所述靶核酸杂交; 在群体的每个寡核苷酸内，T1和T2区域不互补; 并且在群体的每个寡核苷酸内，T1区域与群体的其他寡核苷酸中的至少一个的T2区域互补。 还提供了一种方法，其包括将标记的寡核苷酸群与靶核酸杂交以产生复合物。