公开(公告)号：US07429630B2

公开(公告)日：2008-09-30

申请号：US11429518

申请日：2006-05-04

申请人： Zuifang Liu ,  James Jain Rodgers ,  Geoffrey Lillie

发明人： Zuifang Liu ,  James Jain Rodgers ,  Geoffrey Lillie

IPC分类号： C08F271/02

CPC分类号： C12Q1/006 ,  C12Q1/002 ,  C12Q1/004 ,  Y10S435/817

摘要： A redox polymer for use in an electrochemical-based sensor includes a hydrophobic polymer backbone (e.g., a hydrophobic poly(methyl methacrylate) polymer backbone) and at least one hydrophilic polymer arm (such as a hydrophilic oligo(N-vinylpyrrolidinone) polymer arm) attached to the hydrophobic polymer backbone. The redox polymer also includes a plurality of redox mediators (e.g., ferrocene-based redox mediators) attached to the at least one hydrophilic polymer arm.

摘要翻译： 用于基于电化学的传感器的氧化还原聚合物包括疏水性聚合物主链（例如，疏水聚（甲基丙烯酸甲酯）聚合物主链）和至少一种亲水聚合物臂（例如亲水性低聚（N-乙烯基吡咯烷酮）聚合物臂） 连接到疏水性聚合物主链上。 氧化还原聚合物还包括连接到至少一个亲水性聚合物臂上的多个氧化还原介体（例如，二茂铁基的氧化还原介体）。

公开(公告)号：US20080035479A1

公开(公告)日：2008-02-14

申请号：US11875681

申请日：2007-10-19

申请人： Zuifang LIU ,  James Rodgers ,  Geoffrey Ulue

发明人： Zuifang LIU ,  James Rodgers ,  Geoffrey Ulue

IPC分类号： C12Q1/26

CPC分类号： C12Q1/006 ,  C12Q1/002 ,  C12Q1/004 ,  Y10S435/817

摘要： A redox polymer for use in an electrochemical-based sensor includes a hydrophobic polymer backbone (e.g., a hydrophobic poly(methyl methacrylate) polymer backbone) and at least one hydrophilic polymer arm (such as a hydrophilic oligo(N-vinylpyrrolidinone) polymer arm) attached to the hydrophobic polymer backbone. The redox polymer also includes a plurality of redox mediators (e.g., ferrocene-based redox mediators) attached to the at least one hydrophilic polymer arm.

摘要翻译： 用于基于电化学的传感器的氧化还原聚合物包括疏水性聚合物主链（例如，疏水聚（甲基丙烯酸甲酯）聚合物主链）和至少一种亲水聚合物臂（例如亲水性低聚（N-乙烯基吡咯烷酮）聚合物臂） 连接到疏水性聚合物主链上。 氧化还原聚合物还包括连接到至少一个亲水性聚合物臂上的多个氧化还原介体（例如，二茂铁基的氧化还原介体）。

公开(公告)号：US20130284609A1

公开(公告)日：2013-10-31

申请号：US13460236

申请日：2012-04-30

申请人： Zuifang LIU ,  Steven Setford ,  Marco Cardosi

发明人： Zuifang LIU ,  Steven Setford ,  Marco Cardosi

IPC分类号： G01N27/327 ,  G01N33/50

CPC分类号： C12Q1/004 ,  C12Q1/005

摘要： A nicotinamide adenine dinucleotide (NAD) polymeric coenzyme for use in enzymatic electrochemical-based sensors includes NAD moieties covalently bound as pendent groups to a polymer backbone. An enzymatic electrochemical-based biosensor includes nicotinamide adenine dinucleotide (NAD) polymeric coenzyme, a polymeric electron transfer agent (e.g., polymeric ferrocene) at least one working electrode, and at least one reference electrode.

摘要翻译： 用于基于酶电化学的传感器中的烟酰胺腺嘌呤二核苷酸（NAD）聚合辅酶包括与聚合物主链共价结合作为侧基的NAD部分。 基于酶电化学的生物传感器包括烟酰胺腺嘌呤二核苷酸（NAD）聚合辅酶，聚合电子转移剂（例如聚合二茂铁）至少一个工作电极和至少一个参比电极。

公开(公告)号：US07588670B2

公开(公告)日：2009-09-15

申请号：US11118507

申请日：2005-04-28

申请人： James Iain Rodgers ,  Zuifang Liu ,  Alan Watson McNeilage ,  Margaret MacLennan ,  James Moffat ,  Geoffrey Lillie ,  Michael MacDonald

发明人： James Iain Rodgers ,  Zuifang Liu ,  Alan Watson McNeilage ,  Margaret MacLennan ,  James Moffat ,  Geoffrey Lillie ,  Michael MacDonald

IPC分类号： G01N27/327

CPC分类号： C12Q1/004

摘要： An enzymatic electrochemical-based sensor includes a substrate and a conductive layer formed from a dried water-miscible conductive ink. The water-miscible conductive ink used to form the conductive layer includes a conductive material, an enzyme, a mediator, and a binding agent and is formulated such that the water-miscible conductive ink is a water-miscible aqueous-based dispersion and the binding agent became operatively water-insoluble upon drying.

摘要翻译： 酶电化学传感器包括基材和由干燥的水混溶性导电油墨形成的导电层。 用于形成导电层的与水可混溶的导电油墨包括导电材料，酶，介体和粘合剂，并且被配制成使得水混溶性导电油墨是水混溶性的基于水的分散体和结合 试剂在干燥时变得可操作地不溶于水。

公开(公告)号：US07572356B2

公开(公告)日：2009-08-11

申请号：US10931724

申请日：2004-08-31

申请人： James Iain Rodgers ,  Zuifang Liu ,  Geoffrey Lillie

发明人： James Iain Rodgers ,  Zuifang Liu ,  Geoffrey Lillie

IPC分类号： G01N27/327

CPC分类号： C12Q1/004

摘要： An electrochemical-based sensor includes an electrode with at least one electrode surface, a film disposed on the electrode surface, and a dialysis membrane disposed on the film. The film includes a redox enzyme and a hydrophilic redox polymer (i.e., a polymer with an attached redox mediator(s)). In addition, the dialysis membrane serves to entrap the redox polymer and redox enzyme in the vicinity of the electrode. Such entrapment is accomplished by employing a redox enzyme and a hydrophilic redox polymer of a sufficiently high molecular weight that they do not pass through the dialysis membrane.

摘要翻译： 电化学传感器包括具有至少一个电极表面的电极，设置在电极表面上的膜和设置在膜上的透析膜。 该膜包括氧化还原酶和亲水性氧化还原聚合物（即具有附着的氧化还原介体的聚合物）。 此外，透析膜用于在电极附近截留氧化还原性聚合物和氧化还原酶。 通过使用氧化还原酶和足够高分子量的亲水性氧化还原聚合物，它们不通过透析膜来实现这种捕获。

公开(公告)号：US20120199498A1

公开(公告)日：2012-08-09

申请号：US13021994

申请日：2011-02-07

申请人： Manuel Alvarez-Icaza ,  Alan McNeilage ,  Zuifang Liu ,  Damian Baskeyfield

发明人： Manuel Alvarez-Icaza ,  Alan McNeilage ,  Zuifang Liu ,  Damian Baskeyfield

IPC分类号： G01N27/26

CPC分类号： G01N27/3272 ,  G01N27/3274

摘要： An electrochemical-based analytical test strip for the determination of an analyte (such as glucose) in a bodily fluid sample (e.g., a whole blood sample) includes an electrically-insulating substrate, at least one working electrode disposed on the electrically-insulating substrate and a graded enzymatic reagent layer disposed on the at least one working electrode. The graded enzymatic reagent layer includes an upper reaction grade that contains an enzyme. The graded enzymatic layer also has a lower spacer grade devoid of the enzyme, the lower spacer grade disposed between the upper reaction grade and the working electrode such that the upper reaction grade is spaced equidistant from the working electrode by the lower spacer grade by a predetermined distance during use of the electrochemical-based analytical test strip. In addition, the upper reaction grade is configured and constituted such that an enzymatic reaction between the enzyme and a bodily fluid sample applied to the electrochemical-based analytical test strip is localized in the upper reaction grade and the lower spacer grade has predetermined mass transport characteristics for a component of the enzymatic reaction that cause mass transport of the component through the lower spacer grade to the working electrode to be slower than mass transport of the analyte through the bodily fluid sample to the upper reaction grade. A method for determining an analyte (such as glucose) in a bodily fluid sample (for example, a whole blood sample) includes applying a bodily fluid sample to such an electrochemical-based analytical test strip.

摘要翻译： 用于测定体液样品（例如全血样品）中的分析物（例如葡萄糖）的基于电化学的分析测试条包括电绝缘基底，设置在电绝缘基底上的至少一个工作电极 以及设置在所述至少一个工作电极上的分级酶试剂层。 分级的酶试剂层包括含有酶的上反应级。 分级酶层还具有较低的不含酶的间隔物级别，下部间隔物级别位于上部反应级别和工作电极之间，使得上部反应级别通过下部间隔物等级与工作电极等距预定的预定 在使用电化学分析测试条时的距离。 此外，上反应级被配置和构成使得酶和施加到基于电化学的分析测试条上的体液样品之间的酶反应定位在上反应级中，下隔离物级具有预定的质量传递特性 对于通过下部间隔物等级将组分质量输送到工作电极的酶反应的组分比通过体液样品的分析物质量输送到上部反应级要慢。 用于测定体液样品（例如，全血样品）中的分析物（例如葡萄糖）的方法包括将体液样品施加到这种基于电化学的分析测试条上。

公开(公告)号：US20060226006A1

公开(公告)日：2006-10-12

申请号：US11118507

申请日：2005-04-28

申请人： James Rodgers ,  Zuifang Liu ,  Alan McNeilage ,  Margaret MacLennan ,  James Moffat ,  Geoffrey Lillie ,  Michael MacDonald

发明人： James Rodgers ,  Zuifang Liu ,  Alan McNeilage ,  Margaret MacLennan ,  James Moffat ,  Geoffrey Lillie ,  Michael MacDonald

IPC分类号： C12Q1/00

CPC分类号： C12Q1/004

摘要： An enzymatic electrochemical-based sensor includes a substrate and a conductive layer formed from a dried water-miscible conductive ink. The water-miscible conductive ink used to form the conductive layer includes a conductive material, an enzyme, a mediator, and a binding agent and is formulated such that the water-miscible conductive ink is a water-miscible aqueous-based dispersion and the binding agent became operatively water-insoluble upon drying.

公开(公告)号：US20060025550A1

公开(公告)日：2006-02-02

申请号：US10900511

申请日：2004-07-28

申请人： Zuifang Liu ,  James Rodgers ,  Geoffrey Lillie

发明人： Zuifang Liu ,  James Rodgers ,  Geoffrey Lillie

IPC分类号： C08F118/02

CPC分类号： C12Q1/006 ,  C12Q1/002 ,  C12Q1/004 ,  Y10S435/817

摘要： A redox polymer for use in an electrochemical-based sensor includes a hydrophobic polymer backbone (e.g., a hydrophobic poly(methyl methacrylate) polymer backbone) and at least one hydrophilic polymer arm (such as a hydrophilic oligo(N-vinylpyrrolidinone) polymer arm) attached to the hydrophobic polymer backbone. The redox polymer also includes a plurality of redox mediators (e.g., ferrocene-based redox mediators) attached to the at least one hydrophilic polymer arm.

公开(公告)号：US07592151B2

公开(公告)日：2009-09-22

申请号：US11875681

申请日：2007-10-19

申请人： Zuifang Liu ,  James Jain Rodgers ,  Geoffrey Ulue

发明人： Zuifang Liu ,  James Jain Rodgers ,  Geoffrey Ulue

IPC分类号： C08F271/02 ,  C12Q1/54

CPC分类号： C12Q1/006 ,  C12Q1/002 ,  C12Q1/004 ,  Y10S435/817

摘要： A redox polymer for use in an electrochemical-based sensor includes a hydrophobic polymer backbone (e.g., a hydrophobic poly(methyl methacrylate) polymer backbone) and at least one hydrophilic polymer arm (such as a hydrophilic oligo(N-vinylpyrrolidinone) polymer arm) attached to the hydrophobic polymer backbone. The redox polymer also includes a plurality of redox mediators (e.g., ferrocene-based redox mediators) attached to the at least one hydrophilic polymer arm.

摘要翻译： 用于基于电化学的传感器的氧化还原聚合物包括疏水性聚合物主链（例如，疏水聚（甲基丙烯酸甲酯）聚合物主链）和至少一种亲水聚合物臂（例如亲水性低聚（N-乙烯基吡咯烷酮）聚合物臂） 连接到疏水性聚合物主链上。 氧化还原聚合物还包括连接到至少一个亲水性聚合物臂上的多个氧化还原介体（例如，二茂铁基的氧化还原介体）。

公开(公告)号：US07351770B2

公开(公告)日：2008-04-01

申请号：US10957441

申请日：2004-09-30

申请人： Zuifang Liu ,  James Iain Rodgers ,  Geoffrey Lillie ,  Marco Fabio Cardosi

发明人： Zuifang Liu ,  James Iain Rodgers ,  Geoffrey Lillie ,  Marco Fabio Cardosi

IPC分类号： C08F271/02

CPC分类号： C12Q1/004

摘要： Ionic hydrophilic high molecular weight redox polymers for use in enzymatic electrochemical-based sensors include a hydrophilic polymer (such as a hydrophilic polymer backbone) with ionic portions (e.g., cationic monomers incorporated in the hydrophilic polymer backbone) and a plurality of attached redox mediators. The redox mediators can be, for example, covalently attached to the hydrophilic polymer in a pendant manner. An exemplary cationic hydrophilic high molecular weight redox polymer is synthesized by co-polymerization of a hydrophilic acrylamide monomer, [2-(acryloyloxy)ethyl]trimethyl ammonium chloride and vinyl ferrocene.

摘要翻译： 用于酶电化学传感器的离子型亲水性高分子量氧化还原聚合物包括具有离子部分的亲水性聚合物（例如亲水性聚合物主链）（例如并入亲水性聚合物主链中的阳离子单体）和多个附着的氧化还原介体。 氧化还原介体可以例如以悬挂的方式与亲水性聚合物共价连接。 通过亲水丙烯酰胺单体[2-（丙烯酰氧基）乙基]三甲基氯化铵和乙烯基二茂铁的共聚合来合成示例性的阳离子亲水性高分子量氧化还原聚合物。