公开(公告)号：US20060263612A1

公开(公告)日：2006-11-23

申请号：US11436664

申请日：2006-05-19

申请人： Shu-Hui Chen ,  Honest Makamba ,  Ya-Yu Hsieh ,  Wang-Chou Sung

发明人： Shu-Hui Chen ,  Honest Makamba ,  Ya-Yu Hsieh ,  Wang-Chou Sung

IPC分类号： B32B9/04 ,  B32B27/00

CPC分类号： C08J7/04 ,  B32B27/00 ,  B32B27/08 ,  B32B27/16 ,  B32B27/18 ,  B32B27/283 ,  B32B27/302 ,  B32B27/308 ,  B32B27/34 ,  B32B27/42 ,  B32B2250/24 ,  B32B2307/552 ,  B32B2307/554 ,  B32B2307/714 ,  B32B2307/7145 ,  B32B2307/728 ,  B32B2535/00 ,  C08J2383/04 ,  Y10T428/31663 ,  Y10T428/31667 ,  Y10T428/31906

摘要： The present invention relates to a hydrophilic surface structure of the non-hydrophilic substrate and the manufacturing method for using the same. The hydrophilic substrate surface structure is fabricated by forming an amphiphilic polymer layer, a cross-linked stacking layer, and a hydrophilic layer in sequence on the surface of a non-hydrophilic substrate. For example, the hydrophobic surface of poly(dimethylsiloxane) (PDMS) can be made from hydrophobic to hydrophilic and the hydrophilicity can be retained for a long period of time and resist protein adsorption. The hydrophilic thin films give long term stability to the PDMS surface by resisting hydrophobicity recovery, which is the major problem with PDMS. The disclosed method can further be used in the immobilization of protein and other molecules. This method can also be used for modifying other substrates which suffer problems of surface instability.

摘要翻译： 本发明涉及非亲水性基材的亲水性表面结构体及其制造方法。 通过在非亲水性基材的表面上依次形成两亲性聚合物层，交联层叠层和亲水层来制造亲水性基材表面结构。 例如，聚（二甲基硅氧烷）（PDMS）的疏水表面可以由疏水性制成亲水性，并且亲水性可以长时间保留并抵抗蛋白质吸附。 亲水薄膜通过抵抗疏水性恢复对PDMS表面产生长期稳定性，这是PDMS的主要问题。 所公开的方法可以进一步用于固定蛋白质和其他分子。 该方法也可以用于改变其它具有表面不稳定性问题的基板。

公开(公告)号：US07670686B2

公开(公告)日：2010-03-02

申请号：US11436664

申请日：2006-05-19

申请人： Shu-Hui Chen ,  Makamba Honest ,  Ya-Yu Hsieh ,  Wang-Chou Sung

发明人： Shu-Hui Chen ,  Makamba Honest ,  Ya-Yu Hsieh ,  Wang-Chou Sung

IPC分类号： B32B25/20

CPC分类号： C08J7/04 ,  B32B27/00 ,  B32B27/08 ,  B32B27/16 ,  B32B27/18 ,  B32B27/283 ,  B32B27/302 ,  B32B27/308 ,  B32B27/34 ,  B32B27/42 ,  B32B2250/24 ,  B32B2307/552 ,  B32B2307/554 ,  B32B2307/714 ,  B32B2307/7145 ,  B32B2307/728 ,  B32B2535/00 ,  C08J2383/04 ,  Y10T428/31663 ,  Y10T428/31667 ,  Y10T428/31906

摘要： The present invention relates to a hydrophilic surface structure of the non-hydrophilic substrate and the manufacturing method for using the same. The hydrophilic substrate surface structure is fabricated by forming an amphiphilic polymer layer, a cross-linked stacking layer, and a hydrophilic layer in sequence on the surface of a non-hydrophilic substrate. For example, the hydrophobic surface of poly(dimethylsiloxane) (PDMS) can be made from hydrophobic to hydrophilic and the hydrophilicity can be retained for a long period of time and resist protein adsorption. The hydrophilic thin films give long term stability to the PDMS surface by resisting hydrophobicity recovery, which is the major problem with PDMS. The disclosed method can further be used in the immobilization of protein and other molecules. This method can also be used for modifying other substrates which suffer problems of surface instability.

摘要翻译： 本发明涉及非亲水性基材的亲水性表面结构体及其制造方法。 通过在非亲水性基材的表面上依次形成两亲性聚合物层，交联层叠层和亲水层来制造亲水性基材表面结构。 例如，聚（二甲基硅氧烷）（PDMS）的疏水表面可以由疏水性制成亲水性，并且亲水性可以长时间保留并抵抗蛋白质吸附。 亲水薄膜通过抵抗疏水性恢复对PDMS表面产生长期稳定性，这是PDMS的主要问题。 所公开的方法可以进一步用于固定蛋白质和其他分子。 该方法也可以用于改变其它具有表面不稳定性问题的基板。

公开(公告)号：US07387765B2

公开(公告)日：2008-06-17

申请号：US10822650

申请日：2004-04-13

申请人： Shu-Hui Chen ,  Wang-Chou Sung ,  Pao-Chi Liao ,  Gwo-Bin Lee

发明人： Shu-Hui Chen ,  Wang-Chou Sung ,  Pao-Chi Liao ,  Gwo-Bin Lee

IPC分类号： B01L3/02 ,  G01N1/10

CPC分类号： H01J49/04 ,  G01N27/44717 ,  Y10T436/24 ,  Y10T436/2575

摘要： The present invention provides an integrated microfluidic electrospray chip system and analytical method thereof, characterized in which the proteinase reaction, solid-phase extraction mechanism, electrophoresis and mass spectrometry are integrated into one system. This system allows continuous, fast and on-line detection and identification of a sample, where the sample is firstly hydrolyzed and desalted, and then introduced into the microfluidic electrospray chip to undergo electrophoresis. Finally, the separated sample is introduced into a mass spectrometer by means of electrospray for continuous detection and identification. This system applies mainly in the identification of biochemical substances, such as proteins.

摘要翻译： 本发明提供了一种集成微流体电喷雾芯片系统及其分析方法，其特征在于将蛋白酶反应，固相萃取机理，电泳和质谱法整合到一个体系中。 该系统允许连续，快速和在线检测和鉴定样品，样品首先水解和脱盐，然后引入微流控电喷雾芯片进行电泳。 最后，通过电喷雾将分离的样品引入质谱仪进行连续检测和鉴定。 该系统主要适用于生物化学物质如蛋白质的鉴定。