公开(公告)号：US20100105112A1

公开(公告)日：2010-04-29

申请号：US12310048

申请日：2007-08-07

申请人： Christian Holtze ,  Jeremy Jon Agresti ,  David A. Weitz ,  Keunho Ahn ,  John Brian Hutchison ,  Andrew Gifiths ,  Abdesiam El Harrak ,  Olive John Miller ,  Jean-Christophe Barat ,  Valorie Taly ,  Michael Ryckelynck ,  Christoph Merton

发明人： Christian Holtze ,  Jeremy Jon Agresti ,  David A. Weitz ,  Keunho Ahn ,  John Brian Hutchison ,  Andrew Gifiths ,  Abdesiam El Harrak ,  Olive John Miller ,  Jean-Christophe Barat ,  Valorie Taly ,  Michael Ryckelynck ,  Christoph Merton

IPC分类号： C12P17/14 ,  C07C237/06 ,  B01F17/00

CPC分类号： B01F17/005 ,  B01F17/0035 ,  C08G65/007 ,  C08G65/332 ,  C08G65/337 ,  C08G81/00 ,  C08G2650/48 ,  C08L71/00 ,  C08L2201/52 ,  C08L2205/05

摘要： Surfactants (e.g., fluorosurfactants) for stabilizing aqueous or hydrocarbon droplets in a fluorophilic continuous phase are presented. In some embodiments, fluorosurfactants include a fluorophilic tail soluble in a fluorophilic (e.g., fluorocarbon) continuous phase, and a headgroup soluble in either an aqueous phase or a lipophilic (e.g., hydrocarbon) phase. The combination of a fluorophilic tail and a headgroup may be chosen so as to create a surfactant with a suitable geometry for forming stabilized reverse emulsion droplets having a disperse aqueous or lipophilic phase in a continuous, fluorophilic phase. In some embodiments, the headgroup is preferably non-ionic and can prevent or limit the adsorption of molecules at the interface between the surfactant and the discontinuous phase. This configuration can allow the droplet to serve, for example, as a reaction site for certain chemical and/or biological reactions. In another embodiment, aqueous droplets are stabilized in a fluorocarbon phase at least in part by the electrostatic attraction of two oppositely charged or polar components, one of which is at least partially soluble in the dispersed phase, the other at least partially soluble in the continuous phase. One component may provide collodial stability of the emulsion, and the other may prevent the adsorption of biomolecules at the interface between a component and the discontinous phase. Advantageously, surfactants and surfactant combinations of the invention may provide sufficient stabilization against coalescence of droplets, without interfering with processes that can be carried out inside the droplets.

摘要翻译： 提出了用于稳定氟离子连续相中的水或碳氢化合物液滴的表面活性剂（如含氟表面活性剂）。 在一些实施方案中，含氟表面活性剂包括可溶于氟亲核（例如，碳氟化合物）连续相的荧光尾部和可溶于水相或亲油（例如烃）相的头团。 可以选择荧光尾巴和头组的组合，以便产生具有合适几何形状的表面活性剂，以在连续的氟亲水相中形成具有分散水性或亲油相的稳定的反相乳液液滴。 在一些实施方案中，头基优选是非离子型的，并且可以防止或限制分子在表面活性剂与不连续相之间的界面处的吸附。 这种构型可以使液滴例如用作某些化学和/或生物反应的反应位点。 在另一个实施方案中，水性液滴至少部分地通过两个相反电荷或极性组分的静电吸引而稳定在碳氟化合物相中，其中之一至少部分地可溶于分散相，另一个至少部分可溶于连续的 相。 一种组分可以提供乳液的胶体稳定性，另一种可以防止生物分子在组分和不连续相之间的界面处的吸附。 有利地，本发明的表面活性剂和表面活性剂组合可以提供足够的稳定性以防止液滴的聚结，而不干扰可在液滴内进行的方法。