公开(公告)号：US06790616B1

公开(公告)日：2004-09-14

申请号：US09856662

申请日：2001-05-24

申请人： Toyoki Moribe ,  Toshihiko Kaneshige

发明人： Toyoki Moribe ,  Toshihiko Kaneshige

IPC分类号： C12Q168

CPC分类号： C12Q1/6881 ,  C12Q1/6834 ,  C12Q2531/113 ,  C12Q2600/156

摘要： This invention provides a method, a kit and a reagent for typing of the HLA class I alleles. Explaining concretely, a single HLA class I antigen or allele is determined by combining PCR amplification using a primer pair which can amplify all HLA-A alleles, all HLA-B alleles or all HLA-C alleles, or which is specific to the common sequence to alleles of the specific group consisting of the specific HLA-A alleles or the specific HLA-B alleles, with reverse hybridization analysis using DNA probes capable of specifically hybridizing with the sequence of al least a specific HLA-A allele, at least a specific HLA-B allele or at least a specific HLA-C allele, which are covalently immobilized on wells of microtiter plates.

摘要翻译： 本发明提供了用于分型HLA I等位基因的方法，试剂盒和试剂。 具体地说，通过使用可以扩增所有HLA-A等位基因，所有HLA-B等位基因或所有HLA-C等位基因或其对于共同序列特异性的引物对组合PCR扩增来确定单个HLA I类抗原或等位基因 至特异性HLA-A等位基因或特异性HLA-B等位基因的特异性组的等位基因，使用能够与至少一种特定HLA-A等位基因的序列特异性杂交的DNA探针进行反向杂交分析，至少具体 HLA-B等位基因或至少一个特异性HLA-C等位基因，其共价固定在微量滴定板的孔上。

公开(公告)号：US08927209B2

公开(公告)日：2015-01-06

申请号：US12528982

申请日：2008-03-01

申请人： Yoshihiko Hamamoto ,  Norio Iizuka ,  Toshiaki Miura ,  Toyoki Moribe ,  Masaaki Oka ,  Shigeru Tamatsukuri

发明人： Yoshihiko Hamamoto ,  Norio Iizuka ,  Toshiaki Miura ,  Toyoki Moribe ,  Masaaki Oka ,  Shigeru Tamatsukuri

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6886 ,  C12Q2523/125 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/154 ,  C12Q2600/156

摘要： The present invention provides a novel method for detection of liver cancer. This method detects high-sensitively, high-specifically, simply and accurately liver cancer, especially that in early stage by identifying and/or quantifying methylation on particular genes and/or their DNA fragments in clinical specimens, and by combining said methylated DNA values with existing tumor marker values and/or DNA amounts in blood. This invention also detects a precancerous lesion, detects a risk of recurrence after treatment of liver cancer, detects malignancy of liver cancer and monitors progression of liver cancer with time by the same method. As for particular genes, BASP1 gene, SPINT2 gene, APC gene, CCND2 gene, CFTR gene, RASSF1 gene and SRD5A2 gene are mentioned.

摘要翻译： 本发明提供一种检测肝癌的新方法。 该方法通过鉴定和/或定量临床标本中特定基因和/或其DNA片段的甲基化，并将所述甲基化DNA值与 血液中存在的肿瘤标志物值和/或DNA量。 本发明还检测癌前病变，检测肝癌治疗后复发的风险，通过相同的方法随时间检测肝癌的恶性程度并监测肝癌的进展。 对于特定基因，提及BASP1基因，SPINT2基因，APC基因，CCND2基因，CFTR基因，RASSF1基因和SRD5A2基因。

公开(公告)号：US20100304372A1

公开(公告)日：2010-12-02

申请号：US12528982

申请日：2008-03-01

申请人： Yoshihiko Hamamoto ,  Norio Iizuka ,  Toshiaki Miura ,  Toyoki Moribe ,  Masaaki Oka ,  Shigeru Tamatsukuri

发明人： Yoshihiko Hamamoto ,  Norio Iizuka ,  Toshiaki Miura ,  Toyoki Moribe ,  Masaaki Oka ,  Shigeru Tamatsukuri

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2523/125 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/154 ,  C12Q2600/156

摘要： The present invention provides a novel method for detection of liver cancer. This method detects high-sensitively, high-specifically, simply and accurately liver cancer, especially that in early stage by identifying and/or quantifying methylation on particular genes and/or their DNA fragments in clinical specimens, and by combining said methylated DNA values with existing tumor marker values and/or DNA amounts in blood. This invention also detects a precancerous lesion, detects a risk of recurrence after treatment of liver cancer, detects malignancy of liver cancer and monitors progression of liver cancer with time by the same method. As for particular genes, BASP1 gene, SPINT2 gene, APC gene, CCND2 gene, CFTR gene, RASSF1 gene and SRD5A2 gene are mentioned.

摘要翻译： 本发明提供一种检测肝癌的新方法。 该方法通过鉴定和/或定量临床标本中特定基因和/或其DNA片段的甲基化，并将所述甲基化DNA值与 血液中存在的肿瘤标志物值和/或DNA量。 本发明还检测癌前病变，检测肝癌治疗后复发的风险，通过相同的方法随时间检测肝癌的恶性程度并监测肝癌的进展。 对于特定基因，提及BASP1基因，SPINT2基因，APC基因，CCND2基因，CFTR基因，RASSF1基因和SRD5A2基因。

公开(公告)号：US20050112624A1

公开(公告)日：2005-05-26

申请号：US10920184

申请日：2004-08-18

申请人： Toyoki Moribe ,  Toshihiko Kaneshige

发明人： Toyoki Moribe ,  Toshihiko Kaneshige

IPC分类号： C12Q1/68 ,  C12Q1/6834 ,  C12Q1/6881 ,  G06F19/00 ,  G01N33/48 ,  G01N33/50

CPC分类号： C12Q1/6881 ,  C12Q1/6834 ,  C12Q2531/113 ,  C12Q2600/156

摘要： This invention provides a method, a kit and a reagent for typing of the HLA class I alleles. Explaining concretely, a single HLA class I antigen or allele is determined by combining PCR amplification using a primer pair which can amplify all HLA-A alleles, all HLA-B alleles or all HLA-C alleles, or which is specific to the common sequence to alleles of the specific group consisting of the specific HLA-A alleles or the specific HLA-B alleles, with reverse hybridization analysis using DNA probes capable of specifically hybridizing with the sequence of al least a specific HLA-A allele, at least a specific HLA-B allele or at least a specific HLA-C allele, which are covalently immobilized on wells of microtiter plates.

摘要翻译： 本发明提供了用于分型HLA I等位基因的方法，试剂盒和试剂。 具体地说，通过使用可以扩增所有HLA-A等位基因，所有HLA-B等位基因或所有HLA-C等位基因或其对于共同序列特异性的引物对组合PCR扩增来确定单个HLA I类抗原或等位基因 至特异性HLA-A等位基因或特异性HLA-B等位基因的特异性组的等位基因，使用能够与至少一种特定HLA-A等位基因的序列特异性杂交的DNA探针进行反向杂交分析，至少具体 HLA-B等位基因或至少一个特异性HLA-C等位基因，其共价固定在微量滴定板的孔上。