Process for producing subculturable lymphokine-producing human T cell
hybridomas
    1.
    发明授权
    Process for producing subculturable lymphokine-producing human T cell hybridomas 失效
    用于产生亚培养的产生淋巴因子的人类T细胞杂交瘤的方法

    公开(公告)号:US4675295A

    公开(公告)日:1987-06-23

    申请号:US574213

    申请日:1984-01-26

    摘要: A process for preparing human T cell hybridomas which are subculturable and produce lymphokines comprising the steps of: (1) treating a human acute leukemia cell with a protein and/or RNA synthesis inhibitor; (2) independently activating a human T cell with a mitogen or antigen; (3) fusing the thus-treated human acute leukemia cell with the thus-activated human T cell in the presence of a fusion accelerator; and (4) isolating the thus-formed hybridoma. An in vivo process for producing lymphokines using the hybridomas is also described.

    摘要翻译: 一种制备可亚培养并产生淋巴因子的人T细胞杂交瘤的方法,包括以下步骤:(1)用蛋白质和/或RNA合成抑制剂处理人急性白血病细胞; (2)用丝裂原或抗原独立活化人T细胞; (3)在融合加速器的存在下将如此治疗的人急性白血病细胞与如此活化的人T细胞融合; 和(4)分离由此形成的杂交瘤。 还描述了使用杂交瘤产生淋巴因子的体内方法。

    Human lymphotoxin
    3.
    发明授权
    Human lymphotoxin 失效
    人淋巴毒素

    公开(公告)号:US5776446A

    公开(公告)日:1998-07-07

    申请号:US243168

    申请日:1994-05-16

    CPC分类号: C07K14/5255 A61K38/00

    摘要: Lymphotoxin-producing human T-cell hybridomas are incubated in a medium containing phorbol myristate acetate, concanavalin-A or a mixture thereof, the resulting cells are fractionated by sucrose density-gradient centrifugation method to isolate a messenger RNA in 12.6S to 14.6S fractions, and a gene containing a part encoding a polypeptide having a lymphotoxin-activity is prepared from the messenger RNA. The gene is represented by the base sequence of the Table 1 as herein given. Using the present gene, a new lymphotoxin having the amino acid sequence (I) or (II) as given herein can be obtained by genetic engineering technology.

    摘要翻译: 产生淋巴毒素的人T细胞杂交瘤在含有佛波酮肉豆蔻酸乙酸酯,刀豆杆菌素A或其混合物的培养基中培养,所得细胞通过蔗糖密度梯度离心法分级,以将12.6S至14.6S级分中的信使RNA ,并且从信使RNA制备含有编码具有淋巴毒素活性的多肽的部分的基因。 该基因由本文给出的表1的碱基序列表示。 使用本发明的基因,可以通过遗传工程技术获得具有本文给出的氨基酸序列(I)或(II)的新的淋巴毒素。

    Method for production of lymphotoxin (TNFB) in cell line A-C5-8
    6.
    发明授权
    Method for production of lymphotoxin (TNFB) in cell line A-C5-8 失效
    在细胞系A-C5-8中产生淋巴毒素(TNFB)的方法

    公开(公告)号:US5403725A

    公开(公告)日:1995-04-04

    申请号:US733974

    申请日:1991-07-22

    CPC分类号: C07K14/5255 A61K38/00

    摘要: Lymphotoxin-producing human T-cell hybridomas are incubated in a medium containing phorbol myristate acetate, concanavalin-A or a mixture thereof, the resulting cells are fractionated by sucrose density-gradient centrifugation method to isolate a messenger RNA in 12.6S to 14.6S fractions, and a gene containing a part encoding a polypeptide having a lymphotoxin-activity is prepared from the messenger RNA. The gene is represented by the nucleotide sequence of the Table 1 as herein given. Using the present gene, a new lymphotoxin having the amino acid sequence (I) or (II) as given herein can be obtained by genetic engineering technology.

    摘要翻译: 产生淋巴毒素的人T细胞杂交瘤在含有佛波酮肉豆蔻酸乙酸酯,刀豆杆菌素A或其混合物的培养基中培养,所得细胞通过蔗糖密度梯度离心法分级,以将12.6S至14.6S级分中的信使RNA 并且从信使RNA制备含有编码具有淋巴毒素活性的多肽的部分的基因。 该基因由本文给出的表1的核苷酸序列表示。 使用本发明的基因,可以通过遗传工程技术获得具有本文给出的氨基酸序列(I)或(II)的新的淋巴毒素。