公开(公告)号：US20050181385A1

公开(公告)日：2005-08-18

申请号：US10947637

申请日：2004-09-22

申请人： Peter Linsley ,  Mao Mao ,  Annette Kim ,  Stephen Friend ,  Steven Bartz ,  Michele Cleary

发明人： Peter Linsley ,  Mao Mao ,  Annette Kim ,  Stephen Friend ,  Steven Bartz ,  Michele Cleary

IPC分类号： C07H21/02 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/68

CPC分类号： C12N15/111 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2320/12

摘要： The invention provides a method for identifying one or more genes in a cell of a cell type which interact with, e.g., modulate the effect of, an agent, e.g., a drug. For example, an identified gene may confer resistance or sensitivity to a drug, i.e., reduces or enhances the effect of the drug. The invention also provides STK6 and TPX2 as a gene that exhibits synthetic lethal interactions with KSP encoding a kinesin-like motor protein, and methods and compositions for treatment of diseases, e.g., cancers, by modulating the expression of STK6 or TPX2 gene and/or the activity of STK6 or TPX2 gene product. The invention also provides genes involved in cellular response to DNA damage, and their therapeutic uses.

摘要翻译： 本发明提供了一种用于鉴定与例如药物的作用相互作用的细胞类型的细胞中的一种或多种基因的方法。 例如，鉴定的基因可赋予药物抗药性或敏感性，即降低或增强药物的作用。 本发明还提供STK6和TPX2作为与编码驱动蛋白样运动蛋白的KSP呈现合成的致死相互作用的基因，以及通过调节STK6或TPX2基因的表达和/或治疗疾病例如癌症的方法和组合物 STK6或TPX2基因产物的活性。 本发明还提供涉及对DNA损伤的细胞反应及其治疗用途的基因。

公开(公告)号：US09322020B2

公开(公告)日：2016-04-26

申请号：US14123822

申请日：2012-06-01

申请人： Yong Ma ,  Duncan Brown ,  Steven Bartz

发明人： Yong Ma ,  Duncan Brown ,  Steven Bartz

IPC分类号： C12N15/113 ,  C07H21/02

CPC分类号： C12N15/1137 ,  C07H21/02 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/317 ,  C12N2310/321 ,  C12N2310/332 ,  C12N2310/3521 ,  C12N2310/3533 ,  C12Y101/01042 ,  C12N2310/322

摘要： The present invention relates to compounds, compositions, and methods for the study, diagnosis, and treatment of traits, diseases and conditions that respond to the modulation of IDH1 and mutant IDH1 gene expression and/or activity, and/or modulate an IDH1 or mutant IDH1 gene expression pathway. Specifically, the invention relates to double-stranded nucleic acid molecules, including small nucleic acid molecules such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules, that are capable of mediating or that mediate RNA interference (RNAi) against IDH1 or mutant IDH1 gene expression.

摘要翻译： 本发明涉及用于研究，诊断和治疗响应于IDH1和突变体IDH1基因表达和/或活性的调节和/或调节IDH1或突变体的性状，疾病和病症的化合物，组合物和方法 IDH1基因表达途径。 具体地说，本发明涉及双链核酸分子，包括小核酸分子，如短干扰核酸（siNA），短干扰RNA（siRNA），双链RNA（dsRNA），微RNA（miRNA） 和短发夹RNA（shRNA）分子，其能够介导或介导针对IDH1或突变体IDH1基因表达的RNA干扰（RNAi）。

公开(公告)号：US20070149468A1

公开(公告)日：2007-06-28

申请号：US10557219

申请日：2004-05-17

申请人： Aimee Jackson ,  Steven Bartz ,  Julja Burchard ,  Janell Schelter ,  Peter Linsley

发明人： Aimee Jackson ,  Steven Bartz ,  Julja Burchard ,  Janell Schelter ,  Peter Linsley

IPC分类号： A61K48/00 ,  C12Q1/68

CPC分类号： C12N15/1137 ,  C12N15/1138 ,  C12N2310/14 ,  C12N2320/10 ,  C12N2320/12

摘要： The invention provides methods and compositions for gene silencing by RNA interference. In particular, the invention provides methods for gene silencing or RNA knockdown using small interfering RNAs (siRNAs) having partial sequence homology to its target gene. The invention also provides methods for identifying common and/or differential responses to a plurality of different siRNAs targeting a gene. The invention also provides methods for evaluating the relative activity of the two strands of an siRNA. The invention further provides methods of designing siRNAs for gene silencing. The invention further provides methods of using siRNAs as therapeutics for treatment of diseases.

摘要翻译： 本发明提供了通过RNA干扰进行基因沉默的方法和组合物。 特别地，本发明提供了使用与其靶基因具有部分序列同源性的小干扰RNA（siRNA）进行基因沉默或RNA敲低的方法。 本发明还提供了鉴定针对基因的多种不同siRNA的常见和/或差异应答的方法。 本发明还提供了评估siRNA两条链的相对活性的方法。 本发明还提供了设计用于基因沉默的siRNA的方法。 本发明还提供了使用siRNA作为治疗疾病的治疗剂的方法。

公开(公告)号：US20140094503A1

公开(公告)日：2014-04-03

申请号：US14123822

申请日：2012-06-01

申请人： Yong Ma ,  Duncan Brown ,  Steven Bartz

发明人： Yong Ma ,  Duncan Brown ,  Steven Bartz

IPC分类号： C12N15/113

CPC分类号： C12N15/1137 ,  C07H21/02 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/317 ,  C12N2310/321 ,  C12N2310/332 ,  C12N2310/3521 ,  C12N2310/3533 ,  C12Y101/01042 ,  C12N2310/322

摘要： The present invention relates to compounds, compositions, and methods for the study, diagnosis, and treatment of traits, diseases and conditions that respond to the modulation of IDH1 and mutant IDH1 gene expression and/or activity, and/or modulate an IDH1 or mutant IDH1 gene expression pathway. Specifically, the invention relates to double-stranded nucleic acid molecules, including small nucleic acid molecules such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules, that are capable of mediating or that mediate RNA interference (RNAi) against IDH1 or mutant IDH1 gene expression.

摘要翻译： 本发明涉及用于研究，诊断和治疗响应于IDH1和突变体IDH1基因表达和/或活性的调节和/或调节IDH1或突变体的性状，疾病和病症的化合物，组合物和方法 IDH1基因表达途径。 具体地说，本发明涉及双链核酸分子，包括小核酸分子，如短干扰核酸（siNA），短干扰RNA（siRNA），双链RNA（dsRNA），微RNA（miRNA） 和短发夹RNA（shRNA）分子，其能够介导或介导针对IDH1或突变体IDH1基因表达的RNA干扰（RNAi）。

公开(公告)号：US20070015135A1

公开(公告)日：2007-01-18

申请号：US11440195

申请日：2006-05-23

申请人： Lee Weng ,  Hongyue Dai ,  Steven Bartz

发明人： Lee Weng ,  Hongyue Dai ,  Steven Bartz

IPC分类号： C12Q1/00

CPC分类号： G01N33/5008 ,  G01N33/5091 ,  G06F19/18 ,  G06F19/20 ,  G06F19/24

摘要： The present invention provides methods and computer programs for detecting variations in measurements of a biological variable, e.g., variations in cell viability, under different conditions, e.g., with or without treatment of a drug, under the treatments of different drugs, or under different environmental conditions. The methods and programs of the invention determine a metric of difference between measurements under different condition, and make use of predetermined errors of the measurements, e.g., errors determined from an error model, to estimate the errors in the metric of difference. Such an approach provides a more accurate estimate of the errors of measurements, which, in turn, provides a more accurate estimate of the error of the difference metric.

摘要翻译： 本发明提供了用于检测生物变量的测量变化的方法和计算机程序，例如在不同条件下，例如，在不同药物的治疗下或在不同环境下，在不同的条件下，例如，有或没有治疗药物时，细胞活力的变化 条件。 本发明的方法和程序确定在不同条件下的测量之间的差异的度量，并且利用预定的测量误差，例如从误差模型确定的误差来估计差异度量中的误差。 这种方法提供了对测量误差的更精确的估计，其反过来提供了差异度量的误差的更准确的估计。