Purification of plasminogen
    3.
    发明授权
    Purification of plasminogen 失效
    纤溶酶原的纯化

    公开(公告)号:US3943245A

    公开(公告)日:1976-03-09

    申请号:US440206

    申请日:1974-02-14

    CPC classification number: C12N9/6435 C12Y304/21007 Y10S530/83 Y10S530/831

    Abstract: The purification of plasminogen from human and non-human mammalian plasma or Cohn Fraction III by modified affinity chromotography using Sepharose-L-lysine with high ionic strength buffer solutions as in ion exchange chromatography is described. A plasminogen is produced in great yield having unexpectedly enhanced activity toward N.sup..alpha.-CBZ-L-lysine p-nitrophenyl ester, enhanced fibrinolytic activity and significantly less contamination by unknown colloidal substances.

    Abstract translation: 描述了通过使用Sepharose-L-赖氨酸与离子交换层析中的高离子强度缓冲溶液的改进的亲和层析法从人和非人哺乳动物血浆或Cohn Fraction III中纯化纤溶酶原。 以大量的产率产生纤溶酶原,意外地增强了对N-CZZ-L-赖氨酸对硝基苯酯的活性,增强的纤维蛋白溶解活性和明显较少的未知胶体物质的污染。

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