Fusion polypeptide comprising NtHMA transporter
    3.
    发明授权
    Fusion polypeptide comprising NtHMA transporter 有权
    包含NtHMA转运蛋白的融合多肽

    公开(公告)号:US08829161B2

    公开(公告)日:2014-09-09

    申请号:US13770702

    申请日:2013-02-19

    IPC分类号: C07K14/415 C07K14/00

    摘要: Various embodiments are directed to transgenic plants, including transgenic tobacco plants and derivative seeds, genetically modified to impede the transport of Cadmium (Cd) from the root system to aerial portions of transgenic plants by reducing the expression levels of HMA-related transporters. Various embodiments are directed to transgenic tobacco plants genetically modified to stably express a RNAi construct encoding RNAi polynucleotides that enable the degradation of endogenous NtHMA RNA variants. Reduced expression of NtHMA transporters in transgenic plants results in substantially reduced content of Cadmium (Cd) in the leaf lamina. Various consumable products that are substantially free or substantially reduced in Cd content can be produced by incorporating leaves derived from transgenic tobacco plants modified to reduce the expression of NtHMA transporters.

    摘要翻译: 各种实施方案涉及转基因植物,包括转基因烟草植物和衍生种子,经遗传修饰以通过降低HMA相关转运蛋白的表达水平来阻止将镉(Cd)从根系转运至转基因植物的空中部分。 各种实施方案涉及经遗传修饰以稳定表达编码能够降解内源NtHMA RNA变体的RNAi多核苷酸的RNAi构建体的转基因烟草植物。 在转基因植物中减少NtHMA转运蛋白的表达导致叶片中镉(Cd)的含量显着降低。 可以通过引入来自经修饰的转基因烟草植物的叶来减少NtHMA转运蛋白的表达,可以产生基本上不含或大大降低Cd含量的各种消费品。