公开(公告)号：US5955729A

公开(公告)日：1999-09-21

申请号：US708341

申请日：1996-09-06

申请人： Randall W. Nelson ,  Jennifer R. Krone ,  Russell Granzow ,  Osten Jansson ,  Stefan Sjolander

发明人： Randall W. Nelson ,  Jennifer R. Krone ,  Russell Granzow ,  Osten Jansson ,  Stefan Sjolander

IPC分类号： G01N27/62 ,  G01N21/27 ,  G01N21/55 ,  G01N33/543 ,  B01D59/44 ,  H01J49/00

CPC分类号： H01J49/0418 ,  G01N21/553 ,  H01J49/164

摘要： The invention provides surface plasmon resonance-mass spectroscopy for the rapid, sensitive and accurate investigation of molecular interactions coupled with the identification and quantification of the same. Methods of the invention include capturing an analyte present within a sample by an interactive surface layer on a real-time interaction analysis sensor, analyzing the analyte by surface plasmon resonance while the analyte is captured by the interactive surface layer, and identifying the captured analyte by desorbing/ionizing the analyte from the interactive surface layer while under vacuum within a mass spectrometer. Devices of the invention include a transparent material, a conductive material capable of supporting surface plasmon resonance affixed to the transparent material, an interactive surface affixed to the conductive material, and a means for exposing the interactive surface to the interior of a mass spectrometer without breaking the vacuum therein.

摘要翻译： 本发明提供表面等离子体共振 - 质谱法，用于快速，灵敏和准确地研究分子相互作用以及其鉴定和定量。 本发明的方法包括通过实时相互作用分析传感器上的交互表面层捕获样品中存在的分析物，通过表面等离子体共振分析分析物，同时通过交互表面层捕获分析物，并通过 在质谱仪内真空下从交互表面层解离/电离分析物。 本发明的装置包括透明材料，能够支撑固定在透明材料上的表面等离子体共振的导电材料，固定在导电材料上的相互作用表面，以及用于将相互作用表面暴露于质谱仪内部而不断裂的装置 其中的真空。

公开(公告)号：US20120264233A1

公开(公告)日：2012-10-18

申请号：US13518017

申请日：2010-12-21

申请人： Osten Jansson

发明人： Osten Jansson

IPC分类号： G01N21/55

CPC分类号： G01N21/553 ,  B01F13/0072 ,  G01N21/05 ,  G01N33/54373 ,  G01N35/1065 ,  G01N35/1095

摘要： The invention is a method for characterizing an interaction in a liquid environment, between at least one species in solution and a target immobilized on a surface of a flow cell. The method comprises the following steps: (a) activating the surface of the flow cell and immobilizing the target thereon; (b) providing, in a flow of liquid, at least one of the species; (c) passing the flow of liquid comprising at least one of the species through the surface of the flow cell which contains the immobilized target; and (d) detecting a result of an interaction between the at least one species and the target using surface plasmon resonance (SPR) technique. The improvement of the method comprises in at least one of steps (a) or (b), inline mixing at least two liquid solutions to generate a mixed solution before it is passed through the surface of the flow cell.

摘要翻译： 本发明是用于表征液体环境中的溶液中的至少一种与固定在流动池表面上的靶的相互作用的方法。 该方法包括以下步骤：（a）活化流动池的表面并将靶固定在其上; （b）在液体流中提供至少一种物种; （c）使包含至少一种物质的液体流通过含有固定化靶的流动池的表面; 和（d）使用表面等离子共振（SPR）技术检测所述至少一个物种与靶标之间的相互作用的结果。 该方法的改进包括在步骤（a）或（b）中的至少一个中，将至少两种液体溶液串联混合以在混合溶液通过流动池的表面之前产生混合溶液。