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公开(公告)号：US20110035839A1
公开(公告)日：2011-02-10
申请号：US12832942
申请日：2010-07-08
申请人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Leuthy , Sara E. Heisel , Yuanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
发明人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Leuthy , Sara E. Heisel , Yuanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
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公开(公告)号：US20070118918A1
公开(公告)日：2007-05-24
申请号：US11545071
申请日：2006-10-10
申请人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizz , Yuanji Zhang , Liang Guo , Linda Lutfiyya
发明人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizz , Yuanji Zhang , Liang Guo , Linda Lutfiyya
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
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公开(公告)号：US20070118917A1
公开(公告)日：2007-05-24
申请号：US11544942
申请日：2006-10-10
申请人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizz , Yuanji Zhang , Liang Guo , Linda Lutfiyya
发明人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizz , Yuanji Zhang , Liang Guo , Linda Lutfiyya
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
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公开(公告)号：US20070094748A1
公开(公告)日：2007-04-26
申请号：US11544961
申请日：2006-10-10
申请人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
发明人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
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5.发明申请公开(公告)号：US20070089188A1
公开(公告)日：2007-04-19
申请号：US11545029
申请日：2006-10-10
申请人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
发明人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
IPC分类号： A01H1/00
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular consrtucts and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
摘要翻译： 本发明提供了分子结构及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子内的基因抑制元件，含有 工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。 还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用的方法。 本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。 还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。
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6.发明申请公开(公告)号：US20100223694A1
公开(公告)日：2010-09-02
申请号：US12726673
申请日：2010-03-18
申请人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Luethy , Sara E. Heisel , Yanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
发明人： Linda Lutfiyya , Liang Guo , Philip W. Miller , Michael H. Luethy , Sara E. Heisel , Yanji Zhang , Edwards Allen , Larry A. Gilbertson , David K. Kovalic , James K. Roberts , Shihshieh Huang , Alessandra Frizzi , Nancy Houmard , Thomas Malvar
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
摘要翻译： 本发明提供了分子构建体及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子嵌入的基因抑制元件，含有 工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。 还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用的方法。 本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。 还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。
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公开(公告)号：US20070089196A1
公开(公告)日：2007-04-19
申请号：US11545106
申请日：2006-10-10
申请人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
发明人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
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公开(公告)号：US20070089193A1
公开(公告)日：2007-04-19
申请号：US11545088
申请日：2006-10-10
申请人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
发明人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
IPC分类号： A01H1/00
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
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公开(公告)号：US20070089189A1
公开(公告)日：2007-04-19
申请号：US11545030
申请日：2006-10-10
申请人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Hovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
发明人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Hovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
IPC分类号： A01H1/00
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
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公开(公告)号：US20070083949A1
公开(公告)日：2007-04-12
申请号：US11544944
申请日：2006-10-10
申请人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
发明人： Shihshieh Huang , Thomas Malvar , Michael Luethy , Philip Miller , Larry Gilbertson , Edwards Allen , Sara Heisel , David Kovalic , James Roberts , Nancy Houmard , Alessandra Frizzi , Yuanji Zhang , Liang Guo , Linda Lutfiyya
CPC分类号： C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
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