公开(公告)号：US20110035839A1

公开(公告)日：2011-02-10

申请号：US12832942

申请日：2010-07-08

申请人： Linda Lutfiyya ,  Liang Guo ,  Philip W. Miller ,  Michael H. Leuthy ,  Sara E. Heisel ,  Yuanji Zhang ,  Edwards Allen ,  Larry A. Gilbertson ,  David K. Kovalic ,  James K. Roberts ,  Shihshieh Huang ,  Alessandra Frizzi ,  Nancy Houmard ,  Thomas Malvar

发明人： Linda Lutfiyya ,  Liang Guo ,  Philip W. Miller ,  Michael H. Leuthy ,  Sara E. Heisel ,  Yuanji Zhang ,  Edwards Allen ,  Larry A. Gilbertson ,  David K. Kovalic ,  James K. Roberts ,  Shihshieh Huang ,  Alessandra Frizzi ,  Nancy Houmard ,  Thomas Malvar

IPC分类号： A01H1/06 ,  C12N15/82 ,  C12N5/10 ,  C12N15/63 ,  A01H5/10 ,  A01H5/00

CPC分类号： C12N15/8218 ,  C12N15/113 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8286 ,  C12N2310/113 ,  Y02A40/162

摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.