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公开(公告)号:US09012614B2
公开(公告)日:2015-04-21
申请号:US13698112
申请日:2011-12-27
申请人: Minsu Ko , Young Cheol Kim , Siseok Lee
发明人: Minsu Ko , Young Cheol Kim , Siseok Lee
CPC分类号: C12N15/101 , C07H21/00 , C12N15/1003 , C12Q1/6806 , C12Q2523/308 , C12Q2527/125 , C12Q2531/113 , C12Q2565/137 , G01N30/90 , G01N2030/8827 , Y10T436/255
摘要: Provided is a method for rapid nucleic acid purification. The method includes allowing the nucleic acid isolated from the biological sample to be adsorbed on a silica membrane; separating contaminants from the nucleic acid-adsorbed silica membrane by using paper chromatography; and eluting only high-purity nucleic acid from the silica membrane from which the contaminants are isolated by using a buffer, or applying at least one selected from direct PCR, RT-PCR, real-time PCR, and real-time RT-PCR to the nucleic acid bound onto the silica membrane.
摘要翻译: 提供了快速核酸纯化的方法。 该方法包括使从生物样品分离的核酸吸附在二氧化硅膜上; 通过使用纸色谱法分离污染物与核酸吸附二氧化硅膜; 并且仅从通过使用缓冲液分离污染物的二氧化硅膜仅洗脱高纯度核酸,或将至少一种选自直接PCR,RT-PCR,实时PCR和实时RT-PCR的方法纯化至 核酸结合到二氧化硅膜上。
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公开(公告)号:US20130137107A1
公开(公告)日:2013-05-30
申请号:US13698112
申请日:2011-12-27
申请人: Minsu Ko , Young Cheol Kim , Siseok Lee
发明人: Minsu Ko , Young Cheol Kim , Siseok Lee
CPC分类号: C12N15/101 , C07H21/00 , C12N15/1003 , C12Q1/6806 , C12Q2523/308 , C12Q2527/125 , C12Q2531/113 , C12Q2565/137 , G01N30/90 , G01N2030/8827 , Y10T436/255
摘要: Provided is a method for rapid nucleic acid purification, and the method for rapid nucleic acid isolation according to the present invention is very useful in diagnosing causes of disease or detecting a target gene; can be used in molecular diagnosis of causes of disease more rapidly and conveniently, as compared with the existing nucleic acid isolation method requiring complicated and special equipment; does not require skills therefor, thereby allowing an ordinary person to personally conduct isolation of nucleic acid for analyzing causes of disease and further solving the existing inconvenience caused by directly going to the hospitals or health clinical centers; and can analyze causes of disease more promptly.
摘要翻译: 提供了快速核酸纯化的方法,根据本发明的快速核酸分离方法在诊断疾病的原因或检测靶基因中非常有用; 与现有的需要复杂和特殊设备的核酸分离方法相比,可以更迅速方便地用于疾病原因的分子诊断; 不需要技能,从而允许普通人亲自进行核酸分离,分析疾病原因,进一步解决直接前往医院或健康临床中心造成的不便之处; 并能及时分析疾病原因。
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3.
公开(公告)号:US20170199996A1
公开(公告)日:2017-07-13
申请号:US15469984
申请日:2017-03-27
申请人: Jaejoon HAN , Jungbae Kim , Seon Min Rhee , Seungju Han , Minsu Ko
发明人: Jaejoon HAN , Jungbae Kim , Seon Min Rhee , Seungju Han , Minsu Ko
摘要: An adaptive updating method of an enrollment database is disclosed. The method may include extracting a first feature vector from an input image of a user, determining whether the input image is to be enrolled in an enrollment database based on the first feature vector, second feature vectors of enrollment images including initial enrollment images enrolled in the enrollment database, and a representative vector representing the initial enrollment images, and enrolling the input image in the enrollment database based on a result of the determining.
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