公开(公告)号：US20130030041A1

公开(公告)日：2013-01-31

申请号：US13560645

申请日：2012-07-27

申请人： Mark Gomelsky ,  Min-Hyung Ryu

发明人： Mark Gomelsky ,  Min-Hyung Ryu

IPC分类号： C12N9/96 ,  C12N15/62 ,  C12N15/63 ,  A61P35/00 ,  C40B30/06 ,  C07K19/00 ,  A61K31/711

CPC分类号： C12N15/62 ,  C07K14/195 ,  C07K2319/60 ,  C12N9/6472 ,  C12N9/6475 ,  C12N9/88 ,  C12Y304/22056 ,  C12Y406/01001 ,  C12Y406/01002

摘要： Methods and constructs are provided for controlling processes in live animals, plants or microbes via genetically engineered near-infrared light-activated or light-inactivated proteins including chimeras including the photosensory modules of bacteriohytochromes and output modules that possess enzymatic activity and/or ability to bind to DMA, RNA, protein, or small molecules. DNA encoding these proteins are introduced as genes into live animals, plants or microbes, where their activities can be turned on by near-infrared light, controlled by the intensity of light, and turned off by near-infrared light of a different wavelength than the activating light. These proteins can regulate diverse cellular processes with high spatial and temporal precision, in a nontoxic manner, often using external light sources. For example, near-Infrared light-activated proteins possessing nucleotidyl cyclase, protein kinase, protease, DNA-binding and RNA-binding activities are useful to control signal transduction, cell apopiosis, proliferation, adhesion, differentiation and other cell processes.

摘要翻译： 提供了方法和构建物，用于通过基因工程的近红外光活化或光灭活的蛋白质包括嵌合体来控制活的动物，植物或微生物中的过程，所述嵌合体包括细菌色素的感光模块和具有酶活性和/或结合能力的输出模块 到DMA，RNA，蛋白质或小分子。 编码这些蛋白质的DNA作为基因引入活的动物，植物或微生物中，其活性可以通过受光强度控制的近红外光照射，并被不同波长的近红外光所关闭 激活光线 这些蛋白质可以通过使用外部光源以无毒的方式调节具有高空间和时间精度的多种细胞过程。 例如，具有核苷酸环化酶，蛋白激酶，蛋白酶，DNA结合和RNA结合活性的近红外光活化蛋白质可用于控制信号转导，细胞凋亡，增殖，粘附，分化和其他细胞过程。

公开(公告)号：US08835399B2

公开(公告)日：2014-09-16

申请号：US13560645

申请日：2012-07-27

申请人： Mark Gomelsky ,  Min-Hyung Ryu

发明人： Mark Gomelsky ,  Min-Hyung Ryu

IPC分类号： C12N15/62 ,  C07K19/00 ,  C07K14/195 ,  C12N9/64

CPC分类号： C12N15/62 ,  C07K14/195 ,  C07K2319/60 ,  C12N9/6472 ,  C12N9/6475 ,  C12N9/88 ,  C12Y304/22056 ,  C12Y406/01001 ,  C12Y406/01002

摘要： Methods and constructs are provided for controlling processes in live animals, plants or microbes via genetically engineered near-infrared light-activated or light-inactivated proteins including chimeras including the photosensory modules of bacteriophytochromes and output modules that possess enzymatic activity and/or ability to bind to DMA, RNA, protein, or small molecules. DNA encoding these proteins are introduced as genes into live animals, plants or microbes, where their activities can be turned on by near-infrared light, controlled by the intensity of light, and turned off by near-infrared light of a different wavelength than the activating light. These proteins can regulate diverse cellular processes with high spatial and temporal precision, in a nontoxic manner, often using external light sources. For example, near-infrared light-activated proteins possessing nucleotidyl cyclase, protein kinase, protease, DNA-binding and RNA-binding activities are useful to control signal transduction, cell apoptosis, proliferation, adhesion, differentiation and other cell processes.

摘要翻译： 提供了方法和构建物，用于通过基因工程的近红外光活化或光灭活的蛋白质来控制活的动物，植物或微生物的过程，包括嵌合体，包括细菌色素的光敏模块和具有酶活性和/或结合能力的输出模块 到DMA，RNA，蛋白质或小分子。 编码这些蛋白质的DNA作为基因引入活的动物，植物或微生物中，其活性可以通过受光强度控制的近红外光照射，并被不同波长的近红外光所关闭 激活光线 这些蛋白质可以通过使用外部光源以无毒的方式调节具有高空间和时间精度的多种细胞过程。 例如，具有核苷酸环化酶，蛋白激酶，蛋白酶，DNA结合和RNA结合活性的近红外光活化蛋白质可用于控制信号转导，细胞凋亡，增殖，粘附，分化和其他细胞过程。