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    ACETYL-COA PRODUCING ENZYMES IN YEAST
    2.
    发明申请
    ACETYL-COA PRODUCING ENZYMES IN YEAST 审中-公开
    在乙烯生产酶的乙酰辅酶A

    公开(公告)号：US20100248233A1

    公开(公告)日：2010-09-30

    申请号：US12670050

    申请日：2008-07-11

    Applicant: Ulrike Maria Müller ,  Liang Wu ,  Lourina Madeleine Raamsdonk ,  Aaron Adriaan Winkler

    Inventor： Ulrike Maria Müller ,  Liang Wu ,  Lourina Madeleine Raamsdonk ,  Aaron Adriaan Winkler

    IPC: C12Q1/68 ,  C12N15/63 ,  C12N1/19 ,  C12P7/16

    CPC classification number: C12Q1/32 ,  C07K14/33 ,  C12N9/001 ,  C12P7/16 ,  Y02E50/10

    Abstract: The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grow on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol.

    Abstract translation: 本发明涉及一种鉴定具有用于将丙酮酸，乙醛或乙酸酯转化成酵母细胞的（细胞溶质）中的乙酰辅酶A的酶活性的异源多肽的方法，包括：a）提供包含至少一个缺失的突变的酵母细胞 选自编码丙酮酸脱羧酶（PDC），乙醛脱氢酶（ALD）和乙酰辅酶A合成酶（ACS）的基因的（PDH）旁路的一个基因; b）用包含异源核苷酸序列的表达载体转化所述突变的酵母细胞，所述异源核苷酸序列编码具有将丙酮酸，乙醛或乙酸转化为乙酰辅酶A的潜在酶活性的候选多肽; c）测试所述重组突变酵母细胞在含有葡萄糖作为唯一碳源的基本培养基上生长的能力，以及d）将所述候选多肽鉴定为异源多肽，其具有将丙酮酸，乙醛或乙酸酯转化成乙酰辅酶A 当所述细胞的生长被观察时，所述酵母细胞的胞质溶胶。 本发明还涉及生产发酵生产如丁醇的方法。

    Knee area protection apparatus having a deployment guiding plate and a retaining band system therefor
    7.
    发明授权
    Knee area protection apparatus having a deployment guiding plate and a retaining band system therefor 失效
    膝部区域保护装置具有展开引导板及其保持带系统

    公开(公告)号：US06378902B2

    公开(公告)日：2002-04-30

    申请号：US09815924

    申请日：2001-03-23

    Applicant: Johann Unger ,  Maria Müller ,  Harald Schuler ,  Michael Helbig

    Inventor： Johann Unger ,  Maria Müller ,  Harald Schuler ,  Michael Helbig

    IPC: B60R21045

    CPC classification number: B60R21/215 ,  B60R21/216 ,  B60R2021/2161 ,  B60R2021/23169

    Abstract: A knee area protection apparatus for the protection of a vehicle occupant is provided which includes a deployment guiding plate and a support plate each having a top transverse edge, a bottom transverse edge, and side edges. The support plate is securable to the vehicle in a lower area of a seat compartment thereof. The knee area protection apparatus also includes retaining bands to retain and guide an air bag sack as it is deployed as well as to retain and guide the deployment guiding plate, which is moved by the deployment of the air bag sack.

    Abstract translation: 提供了一种用于保护车辆乘客的膝盖区域保护装置，其包括展开引导板和支撑板，每个支撑板具有顶部横向边缘，底部横向边缘和侧边缘。 支撑板在其座舱的下部区域中可固定到车辆。 膝盖区域保护装置还包括保持带，用于在其展开时保持和引导气囊袋，并且保持和引导通过展开气囊袋而移动的展开导向板。

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