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1.发明申请PRODUCTION OF HIGH TRYPTOPHAN MAIZE BY CHLOROPLAST TARGETED EXPRESSION OF ANTHRANILATE SYNTHASE 审中-公开通过氯仿合成酶的氯化物指示表达生产高三聚磷酸公开(公告)号：US20120246762A1
公开(公告)日：2012-09-27
申请号：US13425415
申请日：2012-03-20
Applicant: Siva Manjunath , Santiago Xavier Navarro , William D. Rapp , Xiaohong Shi , Marguerite J. Varagona , Jennifer L. Winson , Guangning Ye
Inventor： Siva Manjunath , Santiago Xavier Navarro , William D. Rapp , Xiaohong Shi , Marguerite J. Varagona , Jennifer L. Winson , Guangning Ye
CPC classification number: C12N9/88 , C12N15/8214 , C12N15/8254
Abstract: Novel expression vectors and constructs encoding a chloroplast transit peptide (CTP) operably linked to a monomeric anthranilate synthase are provided. Additionally, novel polynucleotide sequences encoding monomeric anthranilate synthases are provided. Also provided are methods for increasing the levels of free tryptophan in transgenic plants containing the expression vectors and constructs.
Abstract translation: 提供了编码与单体邻氨基苯甲酸合酶可操作地连接的叶绿体转运肽（CTP）的新型表达载体和构建体。 另外，提供了编码单体邻氨基苯甲酸合酶的新型多核苷酸序列。 还提供了增加含有表达载体和构建体的转基因植物中游离色氨酸水平的方法。
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Patent Agency Ranking
公开(公告)号：US20110195175A1
公开(公告)日：2011-08-11
申请号：US12685608
申请日：2010-01-11
Applicant: Lisa M. Weaver , Tim N. Oulmassov , Gabriela Vaduva , Jihong Liang , Marguerite J. Varagona , Tyamagondlu V. Venkatesh , Sivalinganna Manjunath
Inventor： Lisa M. Weaver , Tim N. Oulmassov , Gabriela Vaduva , Jihong Liang , Marguerite J. Varagona , Tyamagondlu V. Venkatesh , Sivalinganna Manjunath
CPC classification number: C12N15/8254 , C12N9/88
Abstract: The present invention provides a method for altering the tryptophan content of a plant by introducing and expressing an isolated DNA segment encoding an anthranilate synthase in the cells of the plant. Transgenic plants transformed with an isolated DNA segment encoding an anthranilate synthase, as well as human or animal food, seeds and progeny derived from these plants, are also provided.
Abstract translation: 本发明提供了通过在植物细胞中引入和表达编码邻氨基苯甲酸合酶的分离的DNA区段来改变植物的色氨酸含量的方法。 还提供了用分离的DNA片段转化的转基因植物，其编码邻氨基苯甲酸合酶，以及人或动物食物，来自这些植物的种子和后代。
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3.发明授权Production of high tryptophan maize by chloroplast targeted expression of anthranilate synthase 有权通过叶绿体靶向表达邻氨基苯甲酸合酶生产高色氨酸玉米公开(公告)号：US08138393B2
公开(公告)日：2012-03-20
申请号：US11836690
申请日：2007-08-09
Applicant: Siva Manjunath , Santiago Xavier Navarro , William D. Rapp , Xiaohong Shi , Marguerite J. Varagona , Jennifer L. Winson , Guangning Ye
Inventor： Siva Manjunath , Santiago Xavier Navarro , William D. Rapp , Xiaohong Shi , Marguerite J. Varagona , Jennifer L. Winson , Guangning Ye
IPC: C12N15/00 , A01H1/00 , C07H21/04 , C07K14/415
CPC classification number: C12N9/88 , C12N15/8214 , C12N15/8254
Abstract: Novel expression vectors and constructs encoding a chloroplast transit peptide (CTP) operably linked to a monomeric anthranilate synthase are provided. Additionally, novel polynucleotide sequences encoding monomeric anthranilate synthases are provided. Also provided are methods for increasing the levels of free tryptophan in transgenic plants containing the expression vectors and constructs.
Abstract translation: 提供了编码与单体邻氨基苯甲酸合酶可操作地连接的叶绿体转运肽（CTP）的新型表达载体和构建体。 另外，提供了编码单体邻氨基苯甲酸合酶的新型多核苷酸序列。 还提供了增加含有表达载体和构建体的转基因植物中游离色氨酸水平的方法。
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4.发明授权Production of high tryptophan maize by chloroplast targeted expression of anthranilate synthase 有权通过叶绿体靶向表达邻氨基苯甲酸合酶生产高色氨酸玉米公开(公告)号：US08981188B2
公开(公告)日：2015-03-17
申请号：US13425415
申请日：2012-03-20
Applicant: Siva Manjunath , Santiago Xavier Navarro , William D. Rapp , Xiaohong Shi , Marguerite J. Varagona , Jennifer L. Winson , Guangning Ye
Inventor： Siva Manjunath , Santiago Xavier Navarro , William D. Rapp , Xiaohong Shi , Marguerite J. Varagona , Jennifer L. Winson , Guangning Ye
IPC: A01H5/00 , A01H5/10 , C12P21/04 , C07H21/04 , C12N15/82 , C12Q1/68 , A23K1/00 , A23L1/212 , A23L1/182 , A23L1/20 , C12N9/88
CPC classification number: C12N9/88 , C12N15/8214 , C12N15/8254
Abstract: Novel expression vectors and constructs encoding a chloroplast transit peptide (CTP) operably linked to a monomeric anthranilate synthase are provided. Additionally, novel polynucleotide sequences encoding monomeric anthranilate synthases are provided. Also provided are methods for increasing the levels of free tryptophan in transgenic plants containing the expression vectors and constructs.
Abstract translation: 提供了编码与单体邻氨基苯甲酸合酶可操作地连接的叶绿体转运肽（CTP）的新型表达载体和构建体。 另外，提供了编码单体邻氨基苯甲酸合酶的新型多核苷酸序列。 还提供了增加含有表达载体和构建体的转基因植物中游离色氨酸水平的方法。
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公开(公告)号：US07714189B2
公开(公告)日：2010-05-11
申请号：US11503532
申请日：2006-08-11
Applicant: Siva Manjunath , Jiexin Peng , Marguerite J. Varagona
Inventor： Siva Manjunath , Jiexin Peng , Marguerite J. Varagona
CPC classification number: C12N9/88 , C12N15/8254
Abstract: The present invention provides a method for altering the tryptophan content of a plant by introducing and expressing an artificial polynucleotide sequence encoding an anthranilate synthase in the cells of the plant. Transgenic plants transformed with an artificial polynucleotide sequence encoding an anthranilate synthase, as well as human or animal food, seeds, and progeny derived from these plants, are also provided.
Abstract translation: 本发明提供了通过在植物细胞中引入并表达编码邻氨基苯甲酸合酶的人工多核苷酸序列来改变植物的色氨酸含量的方法。 还提供了用编码邻氨基苯甲酸合酶的人工多核苷酸序列转化的转基因植物，以及来自这些植物的人或动物食物，种子和后代。
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公开(公告)号：US08158851B2
公开(公告)日：2012-04-17
申请号：US12685608
申请日：2010-01-11
Applicant: Lisa M. Weaver , Tim N. Oulmassov , Gabriela Vaduva , Jihong Liang , Marguerite J. Varagona , Tyamagondlu V. Venkatesh , Sivalinganna Manjunath
Inventor： Lisa M. Weaver , Tim N. Oulmassov , Gabriela Vaduva , Jihong Liang , Marguerite J. Varagona , Tyamagondlu V. Venkatesh , Sivalinganna Manjunath
CPC classification number: C12N15/8254 , C12N9/88
Abstract: The present invention provides a method for altering the tryptophan content of a plant by introducing and expressing an isolated DNA segment encoding an anthranilate synthase in the cells of the plant. Transgenic plants transformed with an isolated DNA segment encoding an anthranilate synthase, as well as human or animal food, seeds and progeny derived from these plants, are also provided.
Abstract translation: 本发明提供了通过在植物细胞中引入和表达编码邻氨基苯甲酸合酶的分离的DNA区段来改变植物的色氨酸含量的方法。 还提供了用分离的DNA片段转化的转基因植物，其编码邻氨基苯甲酸合酶，以及人或动物食物，来自这些植物的种子和后代。
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