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1.发明授权公开(公告)号：US08173416B2
公开(公告)日：2012-05-08
申请号：US10475840
申请日：2002-04-23
申请人： Herbert Müller-Hartmann , Gudula Riemen , Kirsten Rothmann-Cosic , Corinna Thiel , Ludger Altrogge , Meike Weigel , Rainer Christine , Elke Lorbach , Juliana Helfrich , Heike Wessendorf , Gregor Siebenkotten
发明人： Herbert Müller-Hartmann , Gudula Riemen , Kirsten Rothmann-Cosic , Corinna Thiel , Ludger Altrogge , Meike Weigel , Rainer Christine , Elke Lorbach , Juliana Helfrich , Heike Wessendorf , Gregor Siebenkotten
CPC分类号： A61N1/327 , A61N1/0412 , C12N15/87 , H03K3/57
摘要： The invention relates to a novel circuit arrangement for electrotransfection or electrofusion, which enables the transportation of DNA and/or other biologically active molecules to the nucleus of higher eukaryotic cells or the fusion of cells, independent of cell division and with reduced cell mortality.
摘要翻译： 本发明涉及一种用于电转移或电融合的新型电路装置，其能够将DNA和/或其他生物活性分子运输到高等真核细胞的细胞核或细胞融合，而与细胞分裂无关并具有降低的细胞死亡率。
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公开(公告)号：US07999073B2
公开(公告)日：2011-08-16
申请号：US11944253
申请日：2007-11-21
申请人： Hanns-Martin Schmidt , Ludger Altrogge , Dietmar Lenz , Gudula Riemen , Helmut Brosterhus , Elke Lorbach , Juliana Helfrich , Katharina Hein , Marion Gremse , Tatjana Males , Rainer Christine , Gregor Siebenkotten , Bodo Ortmann , Tamara Grabek , Andrea Mueller-Hartmann
发明人： Hanns-Martin Schmidt , Ludger Altrogge , Dietmar Lenz , Gudula Riemen , Helmut Brosterhus , Elke Lorbach , Juliana Helfrich , Katharina Hein , Marion Gremse , Tatjana Males , Rainer Christine , Gregor Siebenkotten , Bodo Ortmann , Tamara Grabek , Andrea Mueller-Hartmann
IPC分类号： C07K14/00
CPC分类号： C07K14/47 , A61K48/00 , C07K2319/035 , C07K2319/09 , C07K2319/10 , C07K2319/21 , C12N15/87
摘要： The present invention relates to a method for transfection of cells using at least one protein capable of forming nucleoprotein filaments, wherein the protein is initially modified with at least one functional component which influences one or more steps of the transfection, the nucleic acid to be transfected is then loaded with the modified protein, whereby the nucleic acid and the protein form a filament-like complex, and this complex is finally added to the cells to be transfected. The invention further relates to a transfection agent consisting of nucleoprotein filaments (NPF), with at least one nucleoprotein filament-forming protein being modified with at least one functional component for the transfection. Furthermore, the present invention relates to the use of the transfection agent according to the invention for producing a drug for gene therapeutic treatment of humans and animals. The present inventions also includes corresponding pharmaceutical preparations, especially for use in gene therapy as well as the use of such transfection agents as component in kits.
摘要翻译： 本发明涉及使用至少一种能够形成核蛋白长丝的蛋白质转染细胞的方法，其中蛋白质最初用至少一种影响转染一个或多个步骤的功能成分修饰，待转染的核酸 然后加载修饰的蛋白质，由此核酸和蛋白质形成丝状复合物，并将该复合物最终加入待转染的细胞中。 本发明还涉及由核蛋白丝（NPF）组成的转染剂，至少一种核蛋白长丝形成蛋白被至少一个用于转染的功能组分修饰。 此外，本发明涉及根据本发明的转染剂用于生产用于人和动物基因治疗的药物的用途。 本发明还包括相应的药物制剂，特别是用于基因治疗以及使用这样的转染剂作为试剂盒中的组分。
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3.发明授权公开(公告)号：US09284525B2
公开(公告)日：2016-03-15
申请号：US13145142
申请日：2010-01-20
CPC分类号： C12M35/02 , C12M23/44 , Y10T29/49117
摘要： The invention relates to a method for the application of at least one voltage pulse to at least two containers fitted with electrodes, by means of which at least one voltage pulse is applied to at least one container, while at least one other container is subjected to a preparation or a post-processing. According to the invention the method comprises mutual exchange of the respective positions of the container to which a voltage pulse has already been applied and the further container. Furthermore, the invention relates to a device (1) for making electrical contact with at least one container fitted with electrodes, with at least one receptacle (3, 7) upon which at least one container can be set, and with at least one contact appliance (8) for making contact with the electrodes of the container. According to the invention at least two receptacles (3, 7) are provided, wherein one receptacle (7) is located at or inside the contact appliance (8), and wherein both receptacles (3, 7) and/or the contact appliance (8) can be moved such that after completion of the movement the other receptacle (3) is located at or inside the contact appliance (8).
摘要翻译： 本发明涉及一种用于将至少一个电压脉冲应用于装配有电极的至少两个容器的方法，通过该电容器将至少一个电压脉冲施加到至少一个容器，同时至少一个其它容器经受 准备或后期处理。 根据本发明，该方法包括相互更换已经施加了电压脉冲的容器的相应位置和另外的容器。 此外，本发明涉及一种用于与装有电极的至少一个容器进行电接触的装置（1），其中至少一个容器（3,7）可以在其上设置至少一个容器，并具有至少一个触点 器具（8），用于与容器的电极接触。 根据本发明，提供了至少两个插座（3,7），其中一个插座（7）位于接触器具（8）中或其内部，并且其中两个插座（3,7）和/或触头装置 8）可以移动，使得在移动完成之后，另一容器（3）位于联系设备（8）中或内部。
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公开(公告)号：US09249384B2
公开(公告)日：2016-02-02
申请号：US13145113
申请日：2010-01-20
IPC分类号： C12M1/42
CPC分类号： C12M35/02
摘要： The invention relates to a method for applying at least one electrical voltage pulse to at least one reaction space by at least partially discharging at least one charge storing unit for storing electrical charges. According to the invention the method comprises applying at least one voltage pulse to a reaction space by at least partially discharging a second charge storing unit and simultaneously charging or partially discharging a first charge storing. Furthermore, the invention relates to a device 1 for applying at least one electrical voltage pulse to at least one reaction space, comprising at least two charge storing units (10, 11) for storing electrical charges and at least one power supply unit (12) for charging the charge storing units (10, 11) and/or at least one discharge device for partially discharging the charge storing units (10, 11). According to the invention it is provided that at least two of the charge storing units (10, 11) form a common storage module which can be discharged at any time.
摘要翻译： 本发明涉及通过至少部分地排放至少一个用于存储电荷的电荷存储单元来将至少一个电压脉冲施加到至少一个反应空间的方法。 根据本发明，该方法包括通过至少部分地排放第二电荷存储单元并同时对第一电荷存储进行充电或部分放电来将至少一个电压脉冲施加到反应空间。 此外，本发明涉及一种用于向至少一个反应空间施加至少一个电压脉冲的装置1，包括用于存储电荷的至少两个电荷存储单元（10,11）和至少一个电源单元（12） 用于对电荷存储单元（10,11）充电和/或用于部分地放电电荷存储单元（10,11）的至少一个放电装置。 根据本发明，提供至少两个电荷存储单元（10,11）形成可在任何时间放电的公共存储模块。
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公开(公告)号：US07320859B2
公开(公告)日：2008-01-22
申请号：US10466368
申请日：2002-01-10
申请人： Hanns-Martin Schmidt , Ludger Altrogge , Dietmar Lenz , Gudula Riemen , Helmut Brosterhus , Elke Lorbach , Juliana Helfrich , Katharina Hein , Marion Gremse , Tatjana Males , Rainer Christine , Gregor Siebenkotten , Bodo Ortmann , Tamara Turbanski , Andreas Klaes
发明人： Hanns-Martin Schmidt , Ludger Altrogge , Dietmar Lenz , Gudula Riemen , Helmut Brosterhus , Elke Lorbach , Juliana Helfrich , Katharina Hein , Marion Gremse , Tatjana Males , Rainer Christine , Gregor Siebenkotten , Bodo Ortmann , Tamara Turbanski , Andreas Klaes
IPC分类号： C12Q1/68
CPC分类号： C07K14/47 , A61K48/00 , C07K2319/035 , C07K2319/09 , C07K2319/10 , C07K2319/21 , C12N15/87
摘要： The present invention relates to a method for transfection of cells using at least one protein capable of forming nucleoprotein filaments, wherein the protein is initially modified with at least one functional component which influences one or more steps of the transfection, the nucleic acid to be transfected is then loaded with the modified protein, whereby the nucleic acid and the protein form a filament-like complex, and this complex is finally added to the cells to be transfected. The invention further relates to a transfection agent consisting of nucleoprotein filaments (NPF), with at least one nucleoprotein filament-forming protein being modified with at least one functional component for the transfection. Furthermore, the present invention relates to the use of the transfection agent according to the invention for producing a drug for gene therapeutic treatment of humans and animals. The present inventions also includes corresponding pharmaceutical preparations, especially for use in gene therapy as well as the use of such transfection agents as component in kits.
摘要翻译： 本发明涉及使用至少一种能够形成核蛋白长丝的蛋白质转染细胞的方法，其中蛋白质最初用至少一种影响转染一个或多个步骤的功能成分修饰，待转染的核酸 然后加载修饰的蛋白质，由此核酸和蛋白质形成丝状复合物，并将该复合物最终加入待转染的细胞中。 本发明还涉及由核蛋白丝（NPF）组成的转染剂，至少一种核蛋白长丝形成蛋白被至少一个用于转染的功能组分修饰。 此外，本发明涉及根据本发明的转染剂用于生产用于人和动物基因治疗的药物的用途。 本发明还包括相应的药物制剂，特别是用于基因治疗以及使用这样的转染剂作为试剂盒中的组分。
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6.发明申请公开(公告)号：US20090023131A1
公开(公告)日：2009-01-22
申请号：US11836656
申请日：2007-08-09
申请人： Herbert Muller-Hartmann , Gudula Riemen , Kirsten Rothmann-Cosic , Corinna Thiel , Ludger Altrogge , Meike Weigel , Rainer Christine , Elke Lorbach , Juliana Helfrich , Heike Wessendorf , Gregor Siebenkotten
发明人： Herbert Muller-Hartmann , Gudula Riemen , Kirsten Rothmann-Cosic , Corinna Thiel , Ludger Altrogge , Meike Weigel , Rainer Christine , Elke Lorbach , Juliana Helfrich , Heike Wessendorf , Gregor Siebenkotten
IPC分类号： C12Q3/00
CPC分类号： A61N1/327 , A61N1/0412 , C12N15/87 , H03K3/57
摘要： The invention relates to a novel circuit arrangement for electrotransfection or electrofusion, which enables the transportation of DNA and/or other biologically active molecules to the nucleus of higher eukaryotic cells or the fusion of cells, independent of cell division and with reduced cell mortality.
摘要翻译： 本发明涉及一种用于电转移或电融合的新型电路装置，其能够将DNA和/或其他生物活性分子运输到高等真核细胞的细胞核或细胞融合，而与细胞分裂无关并具有降低的细胞死亡率。
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公开(公告)号：US09080139B2
公开(公告)日：2015-07-14
申请号：US13145124
申请日：2010-01-20
摘要： The invention relates to a container (1) with at least three reaction spaces (2) which in each case have at least one electrode pair for applying an electric voltage for generating an electric field within the reaction space (2) and which are arranged geometrically in at least one row and/or electrically connected in at least one row, wherein at least one electrode (3, 4) of a reaction space (2) is a common electrode (3, 4) with at least one other reaction space (2). According to the invention n+x electrodes (3, 4, 5) are provided, wherein n is the number of reaction spaces (2), with n≧3 and x is the number of rows, with x≧1.
摘要翻译： 本发明涉及具有至少三个反应空间（2）的容器（1），每个反应空间（2）在每种情况下具有至少一个电极对，用于施加用于在反应空间（2）内产生电场的电压并且几何地布置 在至少一排中和/或电连接在至少一排中，其中反应空间（2）的至少一个电极（3,4）是具有至少一个其它反应空间的公共电极（3,4） 2）。 根据本发明，提供n + x电极（3,4,5），其中n是反应空间（2）的数量，n≥3，x是行数，x≥1。
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公开(公告)号：US20120087841A1
公开(公告)日：2012-04-12
申请号：US13145124
申请日：2010-01-20
摘要： The invention relates to a container (1) with at least three reaction spaces (2) which in each case have at least one electrode pair for applying an electric voltage for generating an electric field within the reaction space (2) and which are arranged geometrically in at least one row and/or electrically connected in at least one row, wherein at least one electrode (3, 4) of a reaction space (2) is a common electrode (3, 4) with at least one other reaction space (2). According to the invention n+x electrodes (3, 4, 5) are provided, wherein n is the number of reaction spaces (2), with n≧3 and x is the number of rows, with x≧1.
摘要翻译： 本发明涉及具有至少三个反应空间（2）的容器（1），每个反应空间（2）在每种情况下具有至少一个电极对，用于施加用于在反应空间（2）内产生电场的电压并且几何地布置 在至少一排中和/或电连接在至少一排中，其中反应空间（2）的至少一个电极（3,4）是具有至少一个其它反应空间的公共电极（3,4） 2）。 根据本发明，提供n + x电极（3,4,5），其中n是反应空间（2）的数量，n≥3，x是行数，x≥1。
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公开(公告)号：US20120077245A1
公开(公告)日：2012-03-29
申请号：US13145113
申请日：2010-01-20
CPC分类号： C12M35/02
摘要： The invention relates to a method for applying at least one electrical voltage pulse to at least one reaction space by at least partially discharging at least one charge storing unit for storing electrical charges. According to the invention the method comprises applying at least one voltage pulse to a reaction space by at least partially discharging a second charge storing unit and simultaneously charging or partially discharging a first charge storing. Furthermore, the invention relates to a device 1 for applying at least one electrical voltage pulse to at least one reaction space, comprising at least two charge storing units (10, 11) for storing electrical charges and at least one power supply unit (12) for charging the charge storing units (10, 11) and/or at least one discharge device for partially discharging the charge storing units (10, 11). According to the invention it is provided that at least two of the charge storing units (10, 11) form a common storage module which can be discharged at any time.
摘要翻译： 本发明涉及通过至少部分地排放至少一个用于存储电荷的电荷存储单元来将至少一个电压脉冲施加到至少一个反应空间的方法。 根据本发明，该方法包括通过至少部分地排放第二电荷存储单元并同时对第一电荷存储进行充电或部分放电来将至少一个电压脉冲施加到反应空间。 此外，本发明涉及一种用于向至少一个反应空间施加至少一个电压脉冲的装置1，包括用于存储电荷的至少两个电荷存储单元（10,11）和至少一个电源单元（12） 用于对电荷存储单元（10,11）充电和/或用于部分地放电电荷存储单元（10,11）的至少一个放电装置。 根据本发明，提供了至少两个电荷存储单元（10,11）形成可以随时放电的公共存储模块。
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10.发明申请A METHOD AND A DEVICE FOR THE ELECTRICAL TREATMENT OF A PLURALITY OF CONTAINERS 有权一种用于电化学处理多种容器的方法和装置公开(公告)号：US20120067639A1
公开(公告)日：2012-03-22
申请号：US13145142
申请日：2010-01-20
CPC分类号： C12M35/02 , C12M23/44 , Y10T29/49117
摘要： The invention relates to a method for the application of at least one voltage pulse to at least two containers fitted with electrodes, by means of which at least one voltage pulse is applied to at least one container, while at least one other container is subjected to a preparation or a post-processing. According to the invention the method comprises mutual exchange of the respective positions of the container to which a voltage pulse has already been applied and the further container. Furthermore, the invention relates to a device (1) for making electrical contact with at least one container fitted with electrodes, with at least one receptacle (3, 7) upon which at least one container can be set, and with at least one contact appliance (8) for making contact with the electrodes of the container. According to the invention at least two receptacles (3, 7) are provided, wherein one receptacle (7) is located at or inside the contact appliance (8), and wherein both receptacles (3, 7) and/or the contact appliance (8) can be moved such that after completion of the movement the other receptacle (3) is located at or inside the contact appliance (8).
摘要翻译： 本发明涉及一种用于将至少一个电压脉冲应用于装配有电极的至少两个容器的方法，通过该电容器将至少一个电压脉冲施加到至少一个容器，同时至少一个其它容器经受 准备或后期处理。 根据本发明，该方法包括相互更换已经施加了电压脉冲的容器的相应位置和另外的容器。 此外，本发明涉及一种用于与装有电极的至少一个容器进行电接触的装置（1），至少一个容器（3,7）可以在其上设置至少一个容器，并具有至少一个触点 器具（8），用于与容器的电极接触。 根据本发明，提供了至少两个插座（3,7），其中一个插座（7）位于接触器具（8）中或其内部，并且其中两个插座（3,7）和/或触头装置 8）可以移动，使得在移动完成之后，另一容器（3）位于联系设备（8）中或内部。
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