公开(公告)号：US07604972B2

公开(公告)日：2009-10-20

申请号：US11702690

申请日：2007-02-05

Applicant: Satoshi Mori ,  Kyoko Higuchi ,  Kazuya Suzuki ,  Naoko Nishizawa ,  Hiromi Nakanishi

Inventor： Satoshi Mori ,  Kyoko Higuchi ,  Kazuya Suzuki ,  Naoko Nishizawa ,  Hiromi Nakanishi

IPC: C12N9/00 ,  C12N9/10 ,  C07K14/00 ,  A01H9/00 ,  A01H5/00

CPC classification number: C12N9/1085 ,  C12N15/8243 ,  C12N15/8271

Abstract: A nicotianamine synthase is isolated and purified. Then the gene of this enzyme is cloned and the base sequence and amino acid sequence thereof are determined. This gene is employed in constructing plants, in particular, grass plants highly tolerant to iron-deficiency. A nicotianamine synthase involved in the mugineic acid biosynthesis pathway; the amino acid sequence thereof; a gene encoding the same; a vector containing this gene; cells transformed by the vector; a process for producing nicotianamine by using the same; plants transformed by the gene encoding the nicotianamine synthase; and an antibody against the nicotianamine syntase.

Abstract translation: 分离和纯化烟碱胺合酶。 然后克隆该酶的基因，确定其碱基序列和氨基酸序列。 该基因用于构建植物，特别是高度耐铁缺乏的草本植物。 涉及木瓜酸生物合成途径的烟碱胺合酶; 其氨基酸序列; 编码该基因的基因; 含有该基因的载体; 由载体转化的细胞; 通过使用它来生产烟碱胺的方法; 由编码烟碱胺合成酶的基因转化的植物; 和针对烟碱胺合成酶的抗体。

公开(公告)号：US07192755B1

公开(公告)日：2007-03-20

申请号：US09674337

申请日：1999-04-30

Applicant: Satoshi Mori ,  Kyoko Higuchi ,  Kazuya Suzuki ,  Naoko Nishizawa ,  Hiromi Nakanishi

Inventor： Satoshi Mori ,  Kyoko Higuchi ,  Kazuya Suzuki ,  Naoko Nishizawa ,  Hiromi Nakanishi

IPC: C12N9/10 ,  C12N9/00 ,  A01H9/00 ,  A01H5/00

CPC classification number: C12N9/1085 ,  C12N15/8243 ,  C12N15/8271

Abstract: A nicotianamine synthase is isolated and purified. Then the gene of this enzyme is cloned and the base sequence and amino acid sequence thereof are determined. This gene is employed in constructing plants, in particular, grass plants highly tolerant to iron deficiency. A nicotianamine synthase involved in the mugineic acid biosynthesis pathway; the amino acid sequence thereof; a gene encoding the same; a vector containing this gene; cells transformed by the vector; a process for producing nicotianamine by using the same; plants transformed by the gene encoding the nicotianamine synthase; and an antibody against the nicotianamine syntase.

公开(公告)号：US07157260B2

公开(公告)日：2007-01-02

申请号：US10281024

申请日：2002-10-25

Applicant: Satoshi Mori ,  Kyoko Higuchi ,  Kazuya Suzuki ,  Naoko Nishizawa ,  Hiromi Nakanishi

Inventor： Satoshi Mori ,  Kyoko Higuchi ,  Kazuya Suzuki ,  Naoko Nishizawa ,  Hiromi Nakanishi

IPC: C12N9/10 ,  C12N9/00 ,  C07K1/00 ,  A01H9/00 ,  A01H5/00

CPC classification number: C12N9/1085 ,  C12Y205/01043

Abstract: A nicotianamine synthase is isolated and purified. Then the gene of this enzyme is cloned and the base sequence and amino acid sequence thereof are determined. This gene is employed in constructing plants, in particular, grass plants highly tolerant to iron deficiency. A nicotianamine synthase involved in the mugineic acid biosynthesis pathway; the amino acid sequence thereof; a gene encoding the same; a vector containing this gene; cells transformed by the vector; a process for producing nicotianamine by using the same; plants transformed by the gene encoding the nicotianamine synthase; and an antibody against the nicotianamine syntase.

Abstract translation: 分离和纯化烟碱胺合酶。 然后克隆该酶的基因，确定其碱基序列和氨基酸序列。 该基因用于构建植物，特别是高度耐铁缺乏的草植物。 涉及木瓜酸生物合成途径的烟碱胺合酶; 其氨基酸序列; 编码该基因的基因; 含有该基因的载体; 由载体转化的细胞; 通过使用它来生产烟碱胺的方法; 由编码烟碱胺合成酶的基因转化的植物; 和针对烟碱胺合成酶的抗体。

公开(公告)号：US20070150981A1

公开(公告)日：2007-06-28

申请号：US11702796

申请日：2007-02-05

Applicant: Satoshi Mori ,  Kyoko Higuchi ,  Kazuya Suzuki ,  Naoko Nishizawa ,  Hiromi Nakanishi

Inventor： Satoshi Mori ,  Kyoko Higuchi ,  Kazuya Suzuki ,  Naoko Nishizawa ,  Hiromi Nakanishi

IPC: A01H1/00 ,  C07H21/04 ,  C12N9/10 ,  C12N15/82 ,  C12N5/04

CPC classification number: C12N9/1085 ,  C12N15/8243 ,  C12N15/8271

Abstract: A nicotianamine synthase is isolated and purified. Then the gene of this enzyme is cloned and the base sequence and amino acid sequence thereof are determined. This gene is employed in constructing plants, in particular, grass plants highly tolerant to iron-deficiency. A nicotianamine synthase involved in the mugineic acid biosynthesis pathway; the amino acid sequence thereof; a gene encoding the same; a vector containing this gene; cells transformed by the vector; a process for producing nicotianamine by using the same; plants transformed by the gene encoding the nicotianamine synthase; and an antibody against the nicotianamine syntase.

Abstract translation: 分离和纯化烟碱胺合酶。 然后克隆该酶的基因，确定其碱基序列和氨基酸序列。 该基因用于构建植物，特别是高度耐铁缺乏的草本植物。 涉及木瓜酸生物合成途径的烟碱胺合酶; 其氨基酸序列; 编码该基因的基因; 含有该基因的载体; 由载体转化的细胞; 通过使用它来生产烟碱胺的方法; 由编码烟碱胺合成酶的基因转化的植物; 和针对烟碱胺合成酶的抗体。

公开(公告)号：US20080233627A1

公开(公告)日：2008-09-25

申请号：US11702690

申请日：2007-02-05

Applicant: Satoshi Mori ,  Kyoko Higuchi ,  Kazuya Suzuki ,  Naoko Nishizawa ,  Hiromi Nakanishi

Inventor： Satoshi Mori ,  Kyoko Higuchi ,  Kazuya Suzuki ,  Naoko Nishizawa ,  Hiromi Nakanishi

IPC: C12N9/88

CPC classification number: C12N9/1085 ,  C12N15/8243 ,  C12N15/8271

Abstract: A nicotianamine synthase is isolated and purified. Then the gene of this enzyme is cloned and the base sequence and amino acid sequence thereof are determined. This gene is employed in constructing plants, in particular, grass plants highly tolerant to iron-deficiency. A nicotianamine synthase involved in the mugineic acid biosynthesis pathway; the amino acid sequence thereof; a gene encoding the same; a vector containing this gene; cells transformed by the vector; a process for producing nicotianamine by using the same; plants transformed by the gene encoding the nicotianamine synthase; and an antibody against the nicotianamine syntase.

Abstract translation: 分离和纯化烟碱胺合酶。 然后克隆该酶的基因，确定其碱基序列和氨基酸序列。 该基因用于构建植物，特别是高度耐铁缺乏的草本植物。 涉及木瓜酸生物合成途径的烟碱胺合酶; 其氨基酸序列; 编码该基因的基因; 含有该基因的载体; 由载体转化的细胞; 通过使用它来生产烟碱胺的方法; 由编码烟碱胺合成酶的基因转化的植物; 和针对烟碱胺合成酶的抗体。

公开(公告)号：US07396668B2

公开(公告)日：2008-07-08

申请号：US11702796

申请日：2007-02-05

Applicant: Satoshi Mori ,  Kyoko Higuchi ,  Kazuya Suzuki ,  Naoko Nishizawa ,  Hiromi Nakanishi

Inventor： Satoshi Mori ,  Kyoko Higuchi ,  Kazuya Suzuki ,  Naoko Nishizawa ,  Hiromi Nakanishi

IPC: C12N9/00 ,  C12N9/10 ,  C07K1/00 ,  A01H9/00 ,  A01H5/00

CPC classification number: C12N9/1085 ,  C12N15/8243 ,  C12N15/8271

Abstract: A nicotianamine synthase is isolated and purified. Then the gene of this enzyme is cloned and the base sequence and amino acid sequence thereof are determined. This gene is employed in constructing plants, in particular, grass plants highly tolerant to iron deficiency. A nicotianamine synthase involved in the mugineic acid biosynthesis pathway; the amino acid sequence thereof; a gene encoding the same; a vector containing this gene; cells transformed by the vector; a process for producing nicotianamine by using the same; plants transformed by the gene encoding the nicotianamine synthase; and an antibody against the nicotianamine syntase.

Abstract translation: 分离和纯化烟碱胺合酶。 然后克隆该酶的基因，确定其碱基序列和氨基酸序列。 该基因用于构建植物，特别是高度耐铁缺乏的草植物。 涉及木瓜酸生物合成途径的烟碱胺合酶; 其氨基酸序列; 编码该基因的基因; 含有该基因的载体; 由载体转化的细胞; 通过使用它来生产烟碱胺的方法; 由编码烟碱胺合成酶的基因转化的植物; 和针对烟碱胺合成酶的抗体。