公开(公告)号：US09115353B2

公开(公告)日：2015-08-25

申请号：US13018255

申请日：2011-01-31

Applicant: Kay Klausing ,  Vincent Smith ,  Min-Jui Richard Shen ,  John Moore ,  Kevin Hall

Inventor： Kay Klausing ,  Vincent Smith ,  Min-Jui Richard Shen ,  John Moore ,  Kevin Hall

IPC: C12Q1/68 ,  C12N15/10 ,  C40B20/04 ,  C12Q1/48

CPC classification number: C12Q1/6874 ,  C12N15/1003 ,  C12Q1/6832 ,  C12Q1/6869 ,  C40B20/04 ,  C12Q2523/319 ,  C12Q2527/125 ,  C12Q2527/127

Abstract: A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution.

Abstract translation: 抑制光诱导的核酸降解的方法包括在存在包含多酚化合物的检测溶液的情况下照射一部分核酸。 检测具有荧光标签的核酸的方法包括用合适波长的光照射核酸的至少一部分以诱导荧光发射并检测荧光发射。 任选地，多酚化合物是没食子酸，其低级烷基酯或其混合物。 试剂盒包括一个或多个核苷酸，能够催化将核苷酸掺入核酸链的酶和适于制备检测溶液的多酚化合物。

公开(公告)号：US20120196758A1

公开(公告)日：2012-08-02

申请号：US13018255

申请日：2011-01-31

Applicant: Kay KLAUSING ,  Vincent Smith ,  Min-Jui Richard Shen ,  John Moore ,  Kevin Hall

Inventor： Kay KLAUSING ,  Vincent Smith ,  Min-Jui Richard Shen ,  John Moore ,  Kevin Hall

IPC: C40B20/04 ,  C40B30/00 ,  C12Q1/68

CPC classification number: C12Q1/6874 ,  C12N15/1003 ,  C12Q1/6832 ,  C12Q1/6869 ,  C40B20/04 ,  C12Q2523/319 ,  C12Q2527/125 ,  C12Q2527/127

Abstract: A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution.

Abstract translation: 抑制光诱导的核酸降解的方法包括在存在包含多酚化合物的检测溶液的情况下照射一部分核酸。 检测具有荧光标签的核酸的方法包括用合适波长的光照射核酸的至少一部分以诱导荧光发射并检测荧光发射。 任选地，多酚化合物是没食子酸，其低级烷基酯或其混合物。 试剂盒包括一个或多个核苷酸，能够催化将核苷酸掺入核酸链的酶和适于制备检测溶液的多酚化合物。