公开(公告)号：US20120264926A1

公开(公告)日：2012-10-18

申请号：US13517372

申请日：2010-12-20

申请人： Jason Chin ,  Kaihang Wang ,  Heinz Neumann

发明人： Jason Chin ,  Kaihang Wang ,  Heinz Neumann

IPC分类号： C07H21/02 ,  C07H1/00

CPC分类号： C12N15/11 ,  C12P21/02

摘要： The invention relates to 16S rRNA comprising a mutation at A1196, and to 16S rRNA further comprising a mutation at C1195 and/or A1197, and to 16S rRNA which comprises (i) C1195A and A1196G; or (ii) C1195T, A1196G and A1197G; or (iii) A1196G and A1197G. The invention also relates to ribosomes comprising such 16S rRNAs and to use of same.

摘要翻译： 本发明涉及包含A1196突变的16S rRNA和进一步包含C1195和/或A1197突变的16S rRNA和16S rRNA，其包含（i）C1195A和A1196G; 或（ii）C1195T，A1196G和A1197G; 或（iii）A1196G和A1197G。 本发明还涉及包含这种16S rRNA的核糖体并使用它们。

公开(公告)号：US20100105565A1

公开(公告)日：2010-04-29

申请号：US12516230

申请日：2007-11-28

申请人： Jason Chin ,  Kaihang Wang ,  Heinz Neumann

发明人： Jason Chin ,  Kaihang Wang ,  Heinz Neumann

IPC分类号： C40B30/00 ,  C07H21/02 ,  C12P21/06 ,  C12N1/21

CPC分类号： C12N15/11 ,  C12N15/111 ,  C12N2320/13 ,  C12P21/02 ,  C40B10/00 ,  C40B40/08

摘要： There is provided a method for evolving an orthogonal rRNA molecule, comprising the steps of: providing one or more libraries of mutant orthogonal rRNA molecules and introducing the libraries into cells such that the orthogonal rRNA is incorporated into ribosomes to provide orthogonal ribosomes; providing one or more orthogonal mRNA molecules which (i) are not translated by natural ribosomes, and (ii) comprise one or more orthogonal mRNA codons; assaying the translation of the of the orthogonal mRNA and selecting the orthogonal rRNA molecules which translate the orthogonal mRNA, wherein the assay in step (c) requires translation of one or more orthogonal mRNA codons in the orthogonal mRNA; and orthogonal ribosomes incorporating such rRNA molecules.

摘要翻译： 提供了用于进化正交rRNA分子的方法，包括以下步骤：提供一个或多个突变正交rRNA分子文库，并将文库引入细胞，使得正交rRNA被并入核糖体中以提供正交核糖体; 提供（i）不被天然核糖体翻译的一个或多个正交mRNA分子，和（ii）包含一个或多个正交mRNA密码子; 测定正交mRNA的翻译并选择翻译正交mRNA的正交rRNA分子，其中步骤（c）中的测定需要在正交mRNA中翻译一个或多个正交mRNA密码子; 并结合这样的rRNA分子的正交核糖体。

公开(公告)号：US08497231B2

公开(公告)日：2013-07-30

申请号：US12516230

申请日：2007-11-28

申请人： Jason Chin ,  Kaihang Wang ,  Heinz Neumann

发明人： Jason Chin ,  Kaihang Wang ,  Heinz Neumann

IPC分类号： C40B50/06 ,  C07H21/02 ,  A61K38/00 ,  C07K4/00

CPC分类号： C12N15/11 ,  C12N15/111 ,  C12N2320/13 ,  C12P21/02 ,  C40B10/00 ,  C40B40/08

摘要： There is provided a method for evolving an orthogonal rRNA molecule, comprising the steps of: providing one or more libraries of mutant orthogonal rRNA molecules and introducing the libraries into cells such that the orthogonal rRNA is incorporated into ribosomes to provide orthogonal ribosomes; providing one or more orthogonal mRNA molecules which (i) are not translated by natural ribosomes, and (ii) comprise one or more orthogonal mRNA codons; assaying the translation of the orthogonal mRNA and selecting the orthogonal rRNA molecules which translate the orthogonal mRNA, wherein the assay in step (c) requires translation of one or more orthogonal mRNA codons in the orthogonal mRNA; and orthogonal ribosomes incorporating such rRNA molecules.

摘要翻译： 提供了用于进化正交rRNA分子的方法，包括以下步骤：提供一个或多个突变正交rRNA分子文库，并将文库引入细胞，使得正交rRNA被并入核糖体中以提供正交核糖体; 提供（i）不被天然核糖体翻译的一个或多个正交mRNA分子，和（ii）包含一个或多个正交mRNA密码子; 测定正交mRNA的翻译并选择翻译正交mRNA的正交rRNA分子，其中步骤（c）中的测定需要在正交mRNA中翻译一个或多个正交的mRNA密码子; 并结合这样的rRNA分子的正交核糖体。