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    PREPARATION METHOD OF RECOMBINANT PROTEIN BY USE OF A FUSION EXPRESSION PARTNER
    4.
    发明申请
    PREPARATION METHOD OF RECOMBINANT PROTEIN BY USE OF A FUSION EXPRESSION PARTNER 审中-公开
    通过使用融合表达伴侣的重组蛋白的制备方法

    公开(公告)号：US20110281300A1

    公开(公告)日：2011-11-17

    申请号：US13108518

    申请日：2011-05-16

    申请人： Jeewon Lee ,  Kyung-Yeon Han ,  Jong-Am Song ,  Jin-Seung Park ,  Hyuk-Seong Seo ,  Keum-Young Ahn ,  Eun-Jung Lee ,  Jong-Hwan Lee ,  Soo-Jung Kwon

    发明人： Jeewon Lee ,  Kyung-Yeon Han ,  Jong-Am Song ,  Jin-Seung Park ,  Hyuk-Seong Seo ,  Keum-Young Ahn ,  Eun-Jung Lee ,  Jong-Hwan Lee ,  Soo-Jung Kwon

    IPC分类号： C12P21/00 ,  C12N15/63 ,  C12N1/21 ,  C12N9/00

    CPC分类号： C12N15/67 ,  C12N15/64

    摘要： The present invention relates to a preparation method using a fusion expression partner. The method includes preparing a polynucleotide encoding a fusion expression partner selected from the group consisting of SlyD (FKBR-type peptidyl prolyl cis-trans isomerase), Crr (glucose-specific phosphotransferase (PTS) enzyme IIA component), RpoS (RNA polymerase sigma factor), PotD (Spermidine/putrescine-binding periplasmic protein), and RpoA (RNA polymerase alpha subunit), and an expression vector linking a polyDNA fragment of a heterologous protein, preparing a transformant by introducing the expression vector into a host cell, inducing the expression of a recombinant protein by culturing a transformant, and obtaining the expression. In the preparation method of the recombinant protein, the heterologous protein may enhance the water-solubility and folding of the recombinant protein, overcome the limitations about the water-solubility and folding which the conventional fusion expression partners have, and be used widely in the production of pharmaceutical and industrial proteins.

    摘要翻译： 本发明涉及使用融合表达配偶体的制备方法。 该方法包括制备编码融合表达配偶体的多核苷酸，其选自SlyD（FKBR型肽基脯氨酰顺反异构酶），Crr（葡萄糖特异性磷酸转移酶（PTS）酶IIA组分），RpoS（RNA聚合酶sigma因子 ），PotD（亚精胺/腐胺结合周质蛋白）和RpoA（RNA聚合酶α亚基），以及连接异源蛋白质的多聚DNA片段的表达载体，通过将表达载体导入宿主细胞来制备转化体，诱导 通过培养转化体表达重组蛋白，获得表达。 在重组蛋白的制备方法中，异源蛋白可增强重组蛋白的水溶性和折叠，克服了常规融合表达伴侣具有的水溶性和折叠的局限性，并广泛用于生产 的药物和工业蛋白质。

    Preparation Method Of Recombinant Protein By Use Of A Fusion Expression Partner
    5.
    发明申请
    Preparation Method Of Recombinant Protein By Use Of A Fusion Expression Partner 审中-公开
    利用融合表达合成子制备重组蛋白的方法

    公开(公告)号：US20100210827A1

    公开(公告)日：2010-08-19

    申请号：US12676982

    申请日：2008-09-05

    申请人： Jeewon Lee ,  Kyung-Yeon Han ,  Jong-Am Song ,  Jin-Seung Park ,  Hyuk-Seong Seo ,  Keum-Young Ahn ,  Eun-Jjung Lee ,  Jong-Hwan Lee ,  Soo-Jung Kwon

    发明人： Jeewon Lee ,  Kyung-Yeon Han ,  Jong-Am Song ,  Jin-Seung Park ,  Hyuk-Seong Seo ,  Keum-Young Ahn ,  Eun-Jjung Lee ,  Jong-Hwan Lee ,  Soo-Jung Kwon

    IPC分类号： C07K14/00 ,  C12P21/00 ,  C12N9/00 ,  C12N1/00 ,  C12N15/63

    CPC分类号： C12N15/67 ,  C12N15/64

    摘要： The present invention relates to a preparation method using a fusion expression partner. The method includes preparing a polynucleotide encoding a fusion expression partner selected from the group consisting of SlyD (FKBR type peptidyl prolyl cis-trans isomerase). Crr (glucose-specific phosphotransferase (PTS) enzyme IIA component). RpoS (RNA polymerase sigma factor) PotD (Spermidine/putrescine-binding periplasmic protein), and RpoA (RNA polymerase alpha subunit), and an expression vector linking a polyDNA fragment of a heterologous protein, preparing a transformant by introducing the expression vector into a host cell, inducing the expression of a recombinant protein by culturing a transformant, and obtaining the expression. In the preparation method of the recombinant protein, the heterologous protein may enhance the water-solubility and folding of the recombinant protein, overcome the limitations about the water-solubility and folding which the conventional fusion expression partners have, and be used widely in the production of pharmaceutical and industrial proteins.

    摘要翻译： 本发明涉及使用融合表达配偶体的制备方法。 该方法包括制备编码选自SlyD（FKBR型肽基脯氨酰顺反异构酶）的融合表达伴侣的多核苷酸。 Crr（葡萄糖特异性磷酸转移酶（PTS）酶IIA成分）。 RpoS（RNA聚合酶sigma因子）PotD（亚精胺/腐胺结合周质蛋白）和RpoA（RNA聚合酶α亚基）和连接异源蛋白质的多聚DNA片段的表达载体，通过将表达载体引入到 宿主细胞，通过培养转化体诱导重组蛋白的表达，获得表达。 在重组蛋白的制备方法中，异源蛋白可增强重组蛋白的水溶性和折叠，克服了常规融合表达伴侣具有的水溶性和折叠的局限性，并广泛用于生产 的药物和工业蛋白质。

    RECOMBINANT FLUORESCENT NANOPARTICLES
    6.
    发明申请
    RECOMBINANT FLUORESCENT NANOPARTICLES 审中-公开
    重组荧光纳米颗粒

    公开(公告)号：US20130142732A1

    公开(公告)日：2013-06-06

    申请号：US13487921

    申请日：2012-06-04

    申请人： Jee-Won Lee ,  Keum-Young Ahn ,  Jin-Seung Park

    发明人： Jee-Won Lee ,  Keum-Young Ahn ,  Jin-Seung Park

    IPC分类号： C07K19/00 ,  A61K49/00 ,  G01N21/64 ,  B82Y15/00

    CPC分类号： A61K49/0047 ,  C07K14/43595 ,  C07K2319/735 ,  G01N2021/6439

    摘要： A recombinant fluorescent protein nanoparticle having high fluorescence intensity and a method of detecting a target material using the same are provided. The protein nanoparticle has higher fluorescence intensity than a fluorescent protein, and is resistant to denaturation of the fluorescent protein at room temperature, thereby having higher structural stability than the fluorescent protein itself. In addition, since a self-assembled protein is used as a fusion partner of the fluorescent protein, the protein nanoparticle is biocompatible and safe. Moreover, when a linker peptide is additionally inserted into the protein nanoparticle, a suitable distance between the self-assembled protein and the fluorescent protein is maintained, thereby considerably increasing fluorescence intensity of the protein nanoparticle. The probe-binding protein nanoparticle can control distances between the fluorescent proteins on the surface thereof, thereby maximizing fluorescence intensity.

    摘要翻译： 提供具有高荧光强度的重组荧光蛋白纳米颗粒和使用其的目标材料的检测方法。 蛋白质纳米颗粒具有比荧光蛋白更高的荧光强度，并且在室温下对荧光蛋白质的变性具有抗性，因此具有比荧光蛋白本身更高的结构稳定性。 另外，由于使用自组装蛋白作为荧光蛋白的融合配偶体，所以蛋白质纳米颗粒是生物相容性和安全性的。 此外，当连接肽另外插入到蛋白质纳米颗粒中时，维持自组装蛋白和荧光蛋白之间的适当距离，从而显着增加蛋白质纳米颗粒的荧光强度。 探针结合蛋白纳米颗粒可以控制其表面上的荧光蛋白之间的距离，从而使荧光强度最大化。