公开(公告)号：US20100210827A1

公开(公告)日：2010-08-19

申请号：US12676982

申请日：2008-09-05

申请人： Jeewon Lee ,  Kyung-Yeon Han ,  Jong-Am Song ,  Jin-Seung Park ,  Hyuk-Seong Seo ,  Keum-Young Ahn ,  Eun-Jjung Lee ,  Jong-Hwan Lee ,  Soo-Jung Kwon

发明人： Jeewon Lee ,  Kyung-Yeon Han ,  Jong-Am Song ,  Jin-Seung Park ,  Hyuk-Seong Seo ,  Keum-Young Ahn ,  Eun-Jjung Lee ,  Jong-Hwan Lee ,  Soo-Jung Kwon

IPC分类号： C07K14/00 ,  C12P21/00 ,  C12N9/00 ,  C12N1/00 ,  C12N15/63

CPC分类号： C12N15/67 ,  C12N15/64

摘要： The present invention relates to a preparation method using a fusion expression partner. The method includes preparing a polynucleotide encoding a fusion expression partner selected from the group consisting of SlyD (FKBR type peptidyl prolyl cis-trans isomerase). Crr (glucose-specific phosphotransferase (PTS) enzyme IIA component). RpoS (RNA polymerase sigma factor) PotD (Spermidine/putrescine-binding periplasmic protein), and RpoA (RNA polymerase alpha subunit), and an expression vector linking a polyDNA fragment of a heterologous protein, preparing a transformant by introducing the expression vector into a host cell, inducing the expression of a recombinant protein by culturing a transformant, and obtaining the expression. In the preparation method of the recombinant protein, the heterologous protein may enhance the water-solubility and folding of the recombinant protein, overcome the limitations about the water-solubility and folding which the conventional fusion expression partners have, and be used widely in the production of pharmaceutical and industrial proteins.

摘要翻译： 本发明涉及使用融合表达配偶体的制备方法。 该方法包括制备编码选自SlyD（FKBR型肽基脯氨酰顺反异构酶）的融合表达伴侣的多核苷酸。 Crr（葡萄糖特异性磷酸转移酶（PTS）酶IIA成分）。 RpoS（RNA聚合酶sigma因子）PotD（亚精胺/腐胺结合周质蛋白）和RpoA（RNA聚合酶α亚基）和连接异源蛋白质的多聚DNA片段的表达载体，通过将表达载体引入到 宿主细胞，通过培养转化体诱导重组蛋白的表达，获得表达。 在重组蛋白的制备方法中，异源蛋白可增强重组蛋白的水溶性和折叠，克服了常规融合表达伴侣具有的水溶性和折叠的局限性，并广泛用于生产 的药物和工业蛋白质。