公开(公告)号：US09540625B2

公开(公告)日：2017-01-10

申请号：US14382462

申请日：2013-04-11

Applicant: Inzymes Biotech AB

Inventor： Uno Carlsson ,  Martin Karlsson

IPC: C12N9/88 ,  C07H21/04 ,  B01D53/84

CPC classification number: C12N9/88 ,  B01D53/84 ,  B01D2255/804 ,  B01D2257/504 ,  C12Y402/01001 ,  Y02A50/2358 ,  Y02C10/02

Abstract: An isolated polypeptide having carbonic anhydrase activity, the sequence of which corresponds to modified human carbonic anhydrase II is described. The isolated polypeptide comprises the mutations A23C, S99C, L202C, C205S and V241C and the polypeptide has increased physical stability compared to wild type carbonic anhydrase II. Further, the polypeptide comprises disulfide bridges between C23 and C202 and/or between C99 and C241.

Abstract translation: 描述了具有碳酸酐酶活性的分离的多肽，其序列对应于修饰的人碳酸酐酶II。 分离的多肽包含突变A23C，S99C，L202C，C205S和V241C，与野生型碳酸酐酶II相比，该多肽具有增加的物理稳定性。 此外，多肽包含C23和C202之间和/或C99和C241之间的二硫键。