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公开(公告)号：US20110262402A1
公开(公告)日：2011-10-27
申请号：US12676638
申请日：2008-09-04
Applicant: Masahiko Kuroda , Masakatsu Takanashi , Katsuko Sudo , Shigeki Yamauchi , Hiroyuki Shirono , Toru Hirado , Kenichi Maeda
Inventor： Masahiko Kuroda , Masakatsu Takanashi , Katsuko Sudo , Shigeki Yamauchi , Hiroyuki Shirono , Toru Hirado , Kenichi Maeda
CPC classification number: A61K35/28
Abstract: Provided is a pharmaceutical composition and a method for the treatment and/or prophylaxis of arthritis, inter alia, rheumatoid arthritis. The pharmaceutical composition comprises human mesenchymal stem cells, and the method comprises administering an effective amount human mesenchymal stem cells to a patient.
Abstract translation: 提供了用于治疗和/或预防关节炎，特别是类风湿性关节炎的药物组合物和方法。 药物组合物包含人间充质干细胞，该方法包括向患者施用有效量的人间充质干细胞。
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2.发明授权Method of assaying or analyzing subtypes of human leukocyte interferons or their antibodies, and antibodies to be used therefor 失效检测或分析人类白细胞介素或其抗体的亚型的方法及其使用的抗体
公开(公告)号：US5240864A
公开(公告)日：1993-08-31
申请号：US621825
申请日：1990-12-04
Applicant: Junichi Koga , Hiroyuki Shirono , Akio Matsuo , Hajime Hiratani
Inventor： Junichi Koga , Hiroyuki Shirono , Akio Matsuo , Hajime Hiratani
IPC: G01N33/53 , C07K14/56 , C07K16/24 , G01N30/88 , G01N33/531
CPC classification number: C07K14/56 , C07K16/249
Abstract: Antiserum which can recognize all subtypes of human leukocyte interferon is prepared from the blood of an animal immunized with partially purified human leukocyte interferon obtained from a culture of human leukocyte stimulated with Sendai virus.The antiserum is added to a column whereon concentrated culture broth of human leukocyte has been immobilized to adsorb impurities, the effluent is added to a column whereon partially purified human leukocyte interferon has been immobilized to adsorb anti-human leukocyte interferon antibody and then the antibody is eluted from the column.The antibody thus obtained recognizes all subtypes of human leukocyte interferon and can be separated by a chromatography to each monoclonal antibody which recognizes a single subtype.Employing these antibodies, the subtypes of human leukocyte interferon or their antibodies in a sample can be analized or assayed.
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公开(公告)号：US08465733B2
公开(公告)日：2013-06-18
申请号：US12740758
申请日：2008-10-27
Applicant: Hiroyuki Shirono , Youngjin Byun , Shoichiro Kamei , Kiwamu Imagawa
Inventor： Hiroyuki Shirono , Youngjin Byun , Shoichiro Kamei , Kiwamu Imagawa
CPC classification number: A61K9/10 , A61K9/0019 , A61K35/28 , A61K35/48 , A61K2035/124 , C12N5/0663 , C12N2500/60 , C12N2501/70
Abstract: Disclosed are a method for producing a frozen pharmaceutical composition prepared from cultured human mesenchymal stem cells and a pharmaceutical composition containing human mesenchymal stem cells. This method is a method for producing a frozen pharmaceutical composition containing human mesenchymal stem cells, which comprises the following steps in this order: (a) adding trypsin to human mesenchymal stem cells in a culture vessel to detach the cells from the surface of the culture vessel; (b) adding a bicarbonate Ringer's solution containing human serum albumin to the detached cells to terminate the reaction with trypsin, and washing the cells with the bicarbonate Ringer's solution containing human serum albumin; (c) suspending the cells in a bicarbonate Ringer's solution containing human serum albumin and dimethyl sulfoxide; (d) putting the resulting suspension in a container which allows freezing of what is contained therein, and sealing the container; and (e) freezing the suspension put in the container.
Abstract translation: 公开了一种由培养的人间充质干细胞制备的冷冻药物组合物和含有人间充质干细胞的药物组合物的方法。 该方法是制备含有人间充质干细胞的冷冻药物组合物的方法，该方法包括以下步骤：（a）将培养容器中的胰蛋白酶加入到人间充质干细胞中，以从细胞表面分离细胞 船只; （b）向分离的细胞中加入含有人血清白蛋白的碳酸氢盐林格氏溶液以终止与胰蛋白酶的反应，并用含有人血清白蛋白的碳酸氢盐林格氏溶液洗涤细胞; （c）将细胞悬浮在含有人血清白蛋白和二甲基亚砜的碳酸氢盐林格氏溶液中; （d）将得到的悬浮液放入容器内容纳冷冻物质的容器内，并密封容器; 和（e）冷冻置于容器中的悬浮液。
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公开(公告)号：US20110274663A1
公开(公告)日：2011-11-10
申请号：US12740758
申请日：2008-10-27
Applicant: Hiroyuki Shirono , Youngjin Byun , Shoichiro Kamei , Kiwamu Imagawa
Inventor： Hiroyuki Shirono , Youngjin Byun , Shoichiro Kamei , Kiwamu Imagawa
IPC: A61K35/12 , A61P37/06 , A61P29/00 , A61K35/28 , C12N5/0775
CPC classification number: A61K9/10 , A61K9/0019 , A61K35/28 , A61K35/48 , A61K2035/124 , C12N5/0663 , C12N2500/60 , C12N2501/70
Abstract: Disclosed are a method for producing a frozen pharmaceutical composition prepared from cultured human mesenchymal stem cells and a pharmaceutical composition containing human mesenchymal stem cells. This method is a method for producing a frozen pharmaceutical composition containing human mesenchymal stem cells, which comprises the following steps in this order: (a) adding trypsin to human mesenchymal stem cells in a culture vessel to detach the cells from the surface of the culture vessel; (b) adding a bicarbonate Ringer's solution containing human serum albumin to the detached cells to terminate the reaction with trypsin, and washing the cells with the bicarbonate Ringer's solution containing human serum albumin; (c) suspending the cells in a bicarbonate Ringer's solution containing human serum albumin and dimethyl sulfoxide; (d) putting the resulting suspension in a container which allows freezing of what is contained therein, and sealing the container; and (e) freezing the suspension put in the container.
Abstract translation: 公开了一种由培养的人间充质干细胞制备的冷冻药物组合物和含有人间充质干细胞的药物组合物的方法。 该方法是制备含有人间充质干细胞的冷冻药物组合物的方法，该方法包括以下步骤：（a）将培养容器中的胰蛋白酶加入到人间充质干细胞中，以从细胞表面分离细胞 船只; （b）向分离的细胞中加入含有人血清白蛋白的碳酸氢盐林格氏溶液以终止与胰蛋白酶的反应，并用含有人血清白蛋白的碳酸氢盐林格氏溶液洗涤细胞; （c）将细胞悬浮在含有人血清白蛋白和二甲基亚砜的碳酸氢盐林格氏溶液中; （d）将得到的悬浮液放入容器内容纳冷冻物质的容器内，并密封容器; 和（e）冷冻置于容器中的悬浮液。
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