公开(公告)号：US20230133641A1

公开(公告)日：2023-05-04

申请号：US17812342

申请日：2022-07-13

Applicant: FIOS THERAPEUTICS, LLC

Inventor： Christopher G. A. McGregor ,  Irina Polejaeva ,  Guerard Byrne

IPC: C12N15/85 ,  A01K67/027

Abstract: Provided herein is the first viable galactosyltransferase (Gal) knock-out sheep having a deletion or mutation of alpha-1,3-galactosyltransferase (GGTA1) gene and methods of making the same. Also provided are methods of screening a biological implant for stimulation of an antibody-mediated inflammatory response to a Gal antigen by implanting the biological implant into a recipient Gal knock-out animal and detecting signs of antibody-mediated inflammatory response in the recipient Gal knock-out animal. Further provided is a method of implanting a biological implant into a human subject by screening a first biological implant for signs of antibody-mediated inflammatory response in a recipient Gal knock-out animal and, upon detecting minimal or no signs of antibody mediated inflammatory response in the recipient Gal knock-out animal, implanting a second biological implant into the human subject, wherein the second biological implant is comparable to the first biological implant.

公开(公告)号：US20250025501A1

公开(公告)日：2025-01-23

申请号：US18709445

申请日：2022-11-14

Applicant: IMANIS LIFE SCIENCES, LLC ,  MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH ,  FIOS THERAPEUTICS LLC

Inventor： Stephen J. Russell ,  Karina Krotova ,  Christopher G.A. McGregor

IPC: A61K35/17 ,  A61K35/766 ,  A61K39/00 ,  A61P35/00 ,  C07K14/725 ,  C07K16/28 ,  C12N5/0783 ,  C12N15/86

Abstract: Provided herein are genetically modified ungulate cells expressing a chimeric antigen receptor. Also provided herein are transgenic ungulate cells that can be used as cell carriers for the delivery of oncolytic viruses in cancer therapy. Also provided are transgenic ungulates comprising these cells, methods of making these cells, and methods of treating cancer by administering these cells to a subject in need thereof. Also provided are transgenic ungulate cells that express a chimeric antigen receptor and uses thereof.