公开(公告)号：US11407991B2

公开(公告)日：2022-08-09

申请号：US15138746

申请日：2016-04-26

Applicant: Exosome Diagnostics, Inc.

Inventor： Wayne Comper ,  Leileata M. Russo ,  Johan Karl Olov Skog

IPC: C07H21/00 ,  C12N15/10 ,  C07H21/02 ,  C07H1/08 ,  C12Q1/6806 ,  C12Q1/68 ,  G01N33/00

Abstract: Methods for extracting high quality nucleic acids from a heterogenous collection of nucleic acid-containing materials from a biological sample are disclosed. The heterogenous collection of nucleic-acid containing materials may contain cells or microvesicles, or both. The extractions obtained by the methods described herein are characterized by high yield and high integrity, making the extracted nucleic acids useful for various applications in which high quality nucleic acid extractions are preferred, e.g., a diagnosis, prognosis, or therapy evaluation for a medical condition.

公开(公告)号：US20210171934A1

公开(公告)日：2021-06-10

申请号：US16893966

申请日：2020-06-05

Applicant: Exosome Diagnostics, Inc.

Inventor： Daniel ENDERLE ,  Aparna RAMACHANDRAN ,  Haoheng YAN ,  Emily BERGHOFF ,  Tai-Fen WEI ,  Mikkel NOERHOLM ,  Johan Karl Olov SKOG

IPC: C12N15/10 ,  C12Q1/6806

Abstract: The invention provides novel methods for isolating microvesicles from a biological sample and for extracting nucleic acids from the microvesicles.

公开(公告)号：US10988755B2

公开(公告)日：2021-04-27

申请号：US14877531

申请日：2015-10-07

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov Skog ,  Leileata M. Russo

IPC: C12N15/10

Abstract: A method for extracting nucleic acids from a biological sample by isolating nucleic acid-containing particles from the biological sample by one or more centrifugation procedures, performing one or more steps to mitigate adverse factors that prevent or might prevent high quality nucleic acid extraction, and extracting nucleic acids from the isolated particles. The centrifugation procedures are performed at a speed not exceeding about 200,000 g. The extracted nucleic acids contain both 18S and 28S rRNA.

公开(公告)号：US20190211106A1

公开(公告)日：2019-07-11

申请号：US16261241

申请日：2019-01-29

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov SKOG ,  Emily BERGHOFF ,  Lori LOGUIDICE

IPC: C07K16/28 ,  C07K14/485 ,  C07K14/71 ,  C12Q1/6886 ,  C12N15/113 ,  G01N33/574

CPC classification number: C07K16/2863 ,  A61K47/6849 ,  C07K14/485 ,  C07K14/71 ,  C07K2317/76 ,  C12N15/1138 ,  C12N2310/14 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/156 ,  G01N33/57492 ,  G01N2333/71 ,  G01N2800/52

Abstract: The present invention features a novel EGFR variant, EGFRvVI, and methods for detecting the novel EGFR variant. The novel EGFR variant is preferentially expressed in some cancers. Methods for detecting EGFRvVI may aid in the diagnosis, prognosis, and therapeutic assessment of a subject.

公开(公告)号：US20160024491A1

公开(公告)日：2016-01-28

申请号：US14877531

申请日：2015-10-07

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olav Skog ,  Leileata M. Russo

IPC: C12N15/10

CPC classification number: C12N15/1003

Abstract: A method for extracting nucleic acids from a biological sample by isolating nucleic acid-containing particles from the biological sample by one or more centrifugation procedures, performing one or more steps to mitigate adverse factors that prevent or might prevent high quality nucleic acid extraction, and extracting nucleic acids from the isolated particles. The centrifugation procedures are performed at a speed not exceeding about 200,000 g. The extracted nucleic acids contain both 18S and 28S rRNA.

公开(公告)号：US20220333203A1

公开(公告)日：2022-10-20

申请号：US17532363

申请日：2021-11-22

Applicant: Exosome Diagnostics, Inc.

Inventor： Leileata M. RUSSO

IPC: C12Q1/6886

Abstract: A method for detecting biomarkers of prostate cancer or other medical condition of the prostate based on the use of microvesicles obtained from urine samples, and the nucleic acids present in the microvesicles. The method disclosed herein are advantageous in that they may be used to support diagnosis, prognosis, monitoring, or therapy selection in lieu of or in conjunction with traditional biopsy-based diagnostics and do not require a digital rectal examination or prostate massage prior to urine sample collection.

公开(公告)号：US11457899B2

公开(公告)日：2022-10-04

申请号：US15515980

申请日：2015-09-30

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Johan Skog ,  Bill Tisel ,  Joseph Baker

IPC: A61B10/00 ,  A61B90/90 ,  B01L3/00

Abstract: In order to address an inability to separate portions of a sample and/or substance, in some embodiments, methods and apparatuses for separating components and/or portions of a sample are provided. For example, a sample collection container may comprise two areas within the container, one which is configured to contain a first volume of the sample, and one which is configured to contain a second volume of the sample. A float device may be configured to seal the portion of the container collecting the first volume from the portion of the container collecting the second volume, after the former portion of the container has been filled. The sample collection container may further comprise a top cap configured to further seal the two portions of the container, and to prevent leakage and/or spilling of the sample from the sample collection container.

公开(公告)号：US20220112555A1

公开(公告)日：2022-04-14

申请号：US17560759

申请日：2021-12-23

Applicant: Exosome Diagnostics, Inc. ,  The Brigham and Women's Hospital

Inventor： Johan Karl Olov SKOG ,  Jamil AZZI

IPC: C12Q1/6876 ,  C12Q1/686

Abstract: The invention relates generally to the use of microvesicle RNA signatures for diagnosis, predicting, and/or to monitor treatment efficacy, including patients who are candidates for renal transplant and/or who have received a renal transplant.

公开(公告)号：US20190093172A1

公开(公告)日：2019-03-28

申请号：US16092358

申请日：2017-04-17

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov SKOG ,  Mikkel NOERHOLM ,  James HURLEY ,  Elena CASTELLANOS-RIZALDOS ,  Kay BRINKMAN

IPC: C12Q1/6886 ,  C12N15/10 ,  C12Q1/686 ,  G16H50/20

Abstract: The present invention relates generally to the field of biomarker analysis, particularly determining gene expression signatures from biological samples, including plasma samples.

公开(公告)号：US20170296155A1

公开(公告)日：2017-10-19

申请号：US15515980

申请日：2015-09-30

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Johan SKOG ,  Bill TISEL ,  Joseph BAKER

IPC: A61B10/00 ,  A61B90/90 ,  B01L3/00

CPC classification number: A61B10/007 ,  A61B90/90 ,  B01L3/50825 ,  B01L2200/0689 ,  B01L2200/087 ,  B01L2200/14 ,  B01L2200/141 ,  B01L2300/021 ,  B01L2300/042 ,  B01L2300/0832 ,  B01L2300/0851 ,  B01L2300/087

Abstract: In order to address an inability to separate portions of a sample and/or substance, in some embodiments, methods and apparatuses for separating components and/or portions of a sample are provided. For example, a sample collection container may comprise two areas within the container, one which is configured to contain a first volume of the sample, and one which is configured to contain a second volume of the sample. A float device may be configured to seal the portion of the container collecting the first volume from the portion of the container collecting the second volume, after the former portion of the container has been filled. The sample collection container may further comprise a top cap configured to further seal the two portions of the container, and to prevent leakage and/or spilling of the sample from the sample collection container.