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公开(公告)号:US08093371B2
公开(公告)日:2012-01-10
申请号:US12488971
申请日:2009-06-22
申请人: Eri Maeta , Kenjiro Mori , Tatsuya Konishi
发明人: Eri Maeta , Kenjiro Mori , Tatsuya Konishi
CPC分类号: C07H21/00
摘要: The present invention provides a method of producing a nucleic acid at high reaction efficiency and high reproducibility with a decreased variation in yield and purity among different reaction lots. A nucleic acid synthesis reaction is carried out on a first solid phase carrier capable of supporting nucleic acid synthesis contained in a solid phase carrier mixture comprising the first solid phase carrier and a second solid phase carrier that does not support nucleic acid synthesis.
摘要翻译: 本发明提供了以高反应效率和高再现性生产核酸的方法,其中不同反应批次中产率和纯度的变化减小。 核酸合成反应在能够支持含有第一固相载体和不支持核酸合成的第二固相载体的固相载体混合物中的核酸合成的第一固相载体上进行。
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2.
公开(公告)号:US20090326210A1
公开(公告)日:2009-12-31
申请号:US12490444
申请日:2009-06-24
申请人: Kenjiro Mori , Eri Maeta , Tatsuya Konishi
发明人: Kenjiro Mori , Eri Maeta , Tatsuya Konishi
IPC分类号: C07H1/00 , C08F220/56
CPC分类号: C08F8/12 , C08F12/22 , C08F12/24 , C08F212/08 , C08F2800/10 , C08F220/58 , C08F212/36 , C08F212/14 , C08F220/56
摘要: The present invention provides porous resin beads containing an aromatic monovinyl compound-divinyl compound-(meth)acrylamide derivative copolymer. Preferably, the copolymer further contains as a structural unit a second aromatic monovinyl compound having a functional group capable of binding to a carboxyl group by a dehydrating condensation reaction.
摘要翻译: 本发明提供了含有芳香族单乙烯基化合物 - 二乙烯基化合物 - (甲基)丙烯酰胺衍生物共聚物的多孔树脂珠粒。 优选地,共聚物还含有具有能够通过脱水缩合反应与羧基结合的官能团的第二芳族单乙烯基化合物作为结构单元。
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3.
公开(公告)号:US08802745B2
公开(公告)日:2014-08-12
申请号:US12883684
申请日:2010-09-16
申请人: Kenjiro Mori , Eri Maeta , Tatsuya Konishi
发明人: Kenjiro Mori , Eri Maeta , Tatsuya Konishi
IPC分类号: C08F8/32 , C08F212/08 , C08F222/30 , C08F212/14 , C08F212/36
CPC分类号: C08F212/08 , C08F8/32 , C08F222/30 , C08F212/14 , C08F212/36
摘要: The present invention relates to a porous resin bead containing a first aromatic monovinyl compound-divinyl compound-(meth)acrylonitrile-second aromatic monovinyl compound copolymer, in which the second aromatic monovinyl compound contains a group capable of binding with a carboxyl group by dehydration condensation reaction, and in which the porous resin bead has a swelling volume in acetonitrile of from 3 to 6 ml/g.
摘要翻译: 本发明涉及含有第一芳族单乙烯基化合物 - 二乙烯基化合物 - (甲基)丙烯腈 - 第二芳族单乙烯基化合物共聚物的多孔树脂珠粒,其中第二芳族单乙烯基化合物含有能够通过脱水缩合与羧基结合的基团 反应,其中多孔树脂珠在乙腈中的溶胀体积为3-6ml / g。
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4.
公开(公告)号:US08653152B2
公开(公告)日:2014-02-18
申请号:US12883501
申请日:2010-09-16
申请人: Kenjiro Mori , Tatsuya Konishi , Eri Maeta
发明人: Kenjiro Mori , Tatsuya Konishi , Eri Maeta
IPC分类号: C08F8/12 , C08F212/08
CPC分类号: C08F212/08 , C08F222/30 , C08F212/14 , C08F212/36
摘要: The present invention relates to a porous resin bead containing a first aromatic monovinyl compound-divinyl compound-(meth)acrylonitrile-second aromatic monovinyl compound copolymer, in which the second aromatic monovinyl compound contains a group capable of binding with a carboxyl group by dehydration condensation reaction, and in which the porous resin bead has a dry volume of from 2 to 3 ml/g.
摘要翻译: 本发明涉及含有第一芳族单乙烯基化合物 - 二乙烯基化合物 - (甲基)丙烯腈 - 第二芳族单乙烯基化合物共聚物的多孔树脂珠粒,其中第二芳族单乙烯基化合物含有能够通过脱水缩合与羧基结合的基团 反应,其中多孔树脂珠的干体积为2-3ml / g。
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5.
公开(公告)号:US20110065822A1
公开(公告)日:2011-03-17
申请号:US12883684
申请日:2010-09-16
申请人: Kenjiro MORI , Eri MAETA , Tatsuya KONISHI
发明人: Kenjiro MORI , Eri MAETA , Tatsuya KONISHI
IPC分类号: C08F220/44 , C07H21/00
CPC分类号: C08F212/08 , C08F8/32 , C08F222/30 , C08F212/14 , C08F212/36
摘要: The present invention relates to a porous resin bead containing a first aromatic monovinyl compound-divinyl compound-(meth)acrylonitrile-second aromatic monovinyl compound copolymer, in which the second aromatic monovinyl compound contains a group capable of binding with a carboxyl group by dehydration condensation reaction, and in which the porous resin bead has a swelling volume in acetonitrile of from 3 to 6 ml/g.
摘要翻译: 本发明涉及含有第一芳族单乙烯基化合物 - 二乙烯基化合物 - (甲基)丙烯腈 - 第二芳族单乙烯基化合物共聚物的多孔树脂珠粒,其中第二芳族单乙烯基化合物含有能够通过脱水缩合与羧基结合的基团 反应,其中多孔树脂珠在乙腈中的溶胀体积为3-6ml / g。
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6.
公开(公告)号:US20110065818A1
公开(公告)日:2011-03-17
申请号:US12883501
申请日:2010-09-16
申请人: Kenjiro MORI , Tatsuya KONISHI , Eri MAETA
发明人: Kenjiro MORI , Tatsuya KONISHI , Eri MAETA
CPC分类号: C08F212/08 , C08F222/30 , C08F212/14 , C08F212/36
摘要: The present invention relates to a porous resin bead containing a first aromatic monovinyl compound-divinyl compound-(meth)acrylonitrile-second aromatic monovinyl compound copolymer, in which the second aromatic monovinyl compound contains a group capable of binding with a carboxyl group by dehydration condensation reaction, and in which the porous resin bead has a dry volume of from 2 to 3 ml/g.
摘要翻译: 本发明涉及含有第一芳族单乙烯基化合物 - 二乙烯基化合物 - (甲基)丙烯腈 - 第二芳族单乙烯基化合物共聚物的多孔树脂珠粒,其中第二芳族单乙烯基化合物含有能够通过脱水缩合与羧基结合的基团 反应,其中多孔树脂珠的干体积为2-3ml / g。
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公开(公告)号:US20090325233A1
公开(公告)日:2009-12-31
申请号:US12488971
申请日:2009-06-22
申请人: Eri MAETA , Kenjiro MORI , Tatsuya KONISHI
发明人: Eri MAETA , Kenjiro MORI , Tatsuya KONISHI
IPC分类号: C12P19/34
CPC分类号: C07H21/00
摘要: The present invention provides a method of producing a nucleic acid at high reaction efficiency and high reproducibility with a decreased variation in yield and purity among different reaction lots. A nucleic acid synthesis reaction is carried out on a first solid phase carrier capable of supporting nucleic acid synthesis contained in a solid phase carrier mixture comprising the first solid phase carrier and a second solid phase carrier that does not support nucleic acid synthesis.
摘要翻译: 本发明提供了以高反应效率和高再现性生产核酸的方法,其中不同反应批次中产率和纯度的变化减小。 核酸合成反应在能够支持含有第一固相载体和不支持核酸合成的第二固相载体的固相载体混合物中的核酸合成的第一固相载体上进行。
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公开(公告)号:US20090291294A1
公开(公告)日:2009-11-26
申请号:US12469738
申请日:2009-05-21
申请人: Kenjiro MORI , Eri MAETA
发明人: Kenjiro MORI , Eri MAETA
IPC分类号: B32B5/16
CPC分类号: C08F212/08 , C08F12/20 , C08F12/22 , Y10T428/254 , C08F226/10 , C08F212/14 , C08F212/36 , C08F220/44 , C08F2222/1013 , C08F226/06 , C08F220/14
摘要: According to the invention, there is provided a solid-phase support for nucleic acid synthesis, which is constituted of a porous copolymer particle containing a multifunctional vinyl monomer unit having two or more vinyl groups in one molecule and a monofunctional vinyl monomer unit having one vinyl group in one molecule, in which the solid-phase support has a swelling volume when soaked in acetonitrile of 3.5 ml/g or more and has a median particle diameter measured by a laser scattering particle size distribution measuring method within a range of from 50 to 120 ρm. According to the solid-phase support for nucleic acid synthesis of the invention, a high-purity nucleic acid can be synthesized with a high yield.
摘要翻译: 根据本发明,提供了一种用于核酸合成的固相载体,其由含有一分子中具有两个或更多个乙烯基的多官能乙烯基单体单元的多孔共聚物颗粒和具有一个乙烯基的单官能乙烯基单体单元构成 在一个分子中组合,其中当固体相载体在乙腈中浸泡3.5ml / g或更高时具有溶胀体积,并且具有通过激光散射粒度分布测量方法测量的中值粒径在50至 120公里。 根据本发明的核酸合成的固相支持,可以高产率合成高纯度核酸。
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公开(公告)号:US08669356B2
公开(公告)日:2014-03-11
申请号:US12908676
申请日:2010-10-20
申请人: Yoshihiro Hayakawa , Masaki Tsukamoto , Kenjiro Mori , Kenjiro Minomi , Eri Maeta , Tatsuya Konishi
发明人: Yoshihiro Hayakawa , Masaki Tsukamoto , Kenjiro Mori , Kenjiro Minomi , Eri Maeta , Tatsuya Konishi
CPC分类号: C07H21/04 , C07B2200/11 , C07C69/40 , C40B80/00 , Y02P20/55
摘要: The invention provides a universal linker capable of synthesizing nucleic acid having a phosphate group at the 3′ terminal, a universal support carrying the linker, and a synthesis method of nucleic acid using the universal support. The linker for solid phase synthesis of nucleic acid contains a compound represented by at least one of the following formulae wherein each symbol is as defined in the specification.
摘要翻译: 本发明提供能够合成在3'末端具有磷酸基团的核酸的通用接头,携带接头的通用载体,以及使用通用载体的核酸的合成方法。 用于固相合成核酸的接头包含由至少一个下式表示的化合物,其中每个符号如说明书中所定义。
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10.
公开(公告)号:US08592542B2
公开(公告)日:2013-11-26
申请号:US12490444
申请日:2009-06-24
申请人: Kenjiro Mori , Eri Maeta , Tatsuya Konishi
发明人: Kenjiro Mori , Eri Maeta , Tatsuya Konishi
CPC分类号: C08F8/12 , C08F12/22 , C08F12/24 , C08F212/08 , C08F2800/10 , C08F220/58 , C08F212/36 , C08F212/14 , C08F220/56
摘要: The present invention provides porous resin beads containing an aromatic monovinyl compound-divinyl compound-(meth)acrylamide derivative copolymer. Preferably, the copolymer further contains as a structural unit a second aromatic monovinyl compound having a functional group capable of binding to a carboxyl group by a dehydrating condensation reaction.
摘要翻译: 本发明提供了含有芳香族单乙烯基化合物 - 二乙烯基化合物 - (甲基)丙烯酰胺衍生物共聚物的多孔树脂珠粒。 优选地,共聚物还含有具有能够通过脱水缩合反应与羧基结合的官能团的第二芳族单乙烯基化合物作为结构单元。
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