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    INCREASED ISOPRENE PRODUCTION USING MEVALONATE KINASE AND ISOPRENE SYNTHASE
    3.
    发明申请
    INCREASED ISOPRENE PRODUCTION USING MEVALONATE KINASE AND ISOPRENE SYNTHASE 审中-公开
    使用梅花激素和异丙肾上腺素酶增加异丙酚生产

    公开(公告)号：US20100184178A1

    公开(公告)日：2010-07-22

    申请号：US12560305

    申请日：2009-09-15

    申请人： Zachary Quinn Beck ,  Anthony Rudolf Calabria ,  Michael Charles Miller ,  Dmitrii V. Vaviline ,  Derek H. Wells

    发明人： Zachary Quinn Beck ,  Anthony Rudolf Calabria ,  Michael Charles Miller ,  Dmitrii V. Vaviline ,  Derek H. Wells

    IPC分类号： C12P5/02 ,  C12N9/12

    CPC分类号： C12P5/007 ,  C12N9/1085 ,  C12N9/1205 ,  C12P7/04 ,  C12Y207/01036

    摘要： The invention features methods for producing isoprene from cultured cells having increased expression levels and/or activity levels of a mevalonate kinase polypeptide and an isoprene synthase polypeptide. The invention also provides methods for producing isoprene from cultured cells having reduced accumulation of intermediates (such as mevalonate, isopentenyl diphosphate, 3,3-dimethylallyl diphosphate, geranyl diphosphate, or farnesyl diphosphate) in the biosynthesis of isoprene or isoprenoids that may otherwise cause undesirable amounts of growth inhibition, toxicity, or cell death. The resulting isoprene compositions may have increased yields and/or purity of isoprene.

    摘要翻译： 本发明的特征在于具有增加的甲羟戊酸激酶多肽和异戊二烯合酶多肽的表达水平和/或活性水平的培养细胞产生异戊二烯的方法。 本发明还提供了在异戊二烯或类异戊二烯的生物合成中具有减少的中间体（例如甲羟戊酸，异戊烯基二磷酸，3,3-二甲基烯丙基二磷酸酯，香叶基二磷酸酯或法呢基二磷酸酯）积累减少的培养细胞的方法。 生长抑制，毒性或细胞死亡的量。 得到的异戊二烯组合物可以具有增加的异戊二烯的产率和/或纯度。