公开(公告)号：US20070172963A1

公开(公告)日：2007-07-26

申请号：US10557300

申请日：2004-06-03

申请人： Gary Krauth ,  David Ledden

发明人： Gary Krauth ,  David Ledden

IPC分类号： G01N33/558

CPC分类号： G01N33/558 ,  G01N33/56911 ,  G01N33/56927 ,  G01N33/56988 ,  G01N33/571 ,  G01N33/6854 ,  G01N33/6887 ,  G01N33/689 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S435/973 ,  Y10S435/975 ,  Y10S436/805 ,  Y10S436/808 ,  Y10S436/81

摘要： This invention is directed to a lateral flow assay for detecting the presence of an analyte in a liquid test sample. The lateral flow assay represents an improvement in the ability to accurately and with high fidelity to detect the presence or absence of a target analyte in a liquid sample, in part, by encompassing a reference region of immobilized, non-diffusible analyte that allows for detection of any factors that interfere with the interaction and binding of the analyte to the labeled capture reagent. Any influences on the interaction and binding of the analyte that is free in solution in the liquid test sample to its complementary labeled reagent will be encountered in parallel in the binding between the immobilized analyte in reference region to the labeled reagent as it diffuses through the reference region. In one embodiment, the lateral flow assay of the invention is a urine-based human Chorionic Gonadotropin (hCG) assay.

摘要翻译： 本发明涉及用于检测液体测试样品中分析物的存在的侧向流动测定。 横向流量测定表示准确地并且以高保真度检测液体样品中目标分析物的存在或不存在的能力的改进，部分地通过包含允许检测的固定的不扩散分析物的参考区域 干扰分析物与标记的捕获试剂的相互作用和结合的任何因素。 在液体测试样品中溶液中游离的分析物与其互补的标记试剂之间的相互作用和结合的任何影响将与参考区域中的固定化分析物与标记试剂的结合并行遇到，因为它通过参考文献扩散 地区。 在一个实施方案中，本发明的侧流测定是基于尿液的人绒毛膜促性腺激素（hCG）测定。

公开(公告)号：US20070278431A1

公开(公告)日：2007-12-06

申请号：US10556097

申请日：2004-06-03

申请人： David Brock ,  Chris Zimmerle ,  David Ledden

发明人： David Brock ,  Chris Zimmerle ,  David Ledden

IPC分类号： G01N21/84

CPC分类号： G01N21/251 ,  G01N21/64 ,  G01N21/8483

摘要： Identifying a test product having a test region includes impinging on the test region a set of test signals at known test wavelengths. An image of the test region is generated from the reflected signals. The image is comprised of a set of reflectance values that are compared against reference reflectance values at the test signal wavelengths to determine the test product type.

摘要翻译： 识别具有测试区域的测试产品包括在测试区域上照射已知测试波长的一组测试信号。 从反射信号产生测试区域的图像。 该图像由与测试信号波长的参考反射率值进行比较以确定测试产品类型的一组反射率值组成。

公开(公告)号：US20070064220A1

公开(公告)日：2007-03-22

申请号：US10556299

申请日：2004-06-03

申请人： Nicholas Stock ,  Gary Ross ,  David Ledden

发明人： Nicholas Stock ,  Gary Ross ,  David Ledden

IPC分类号： G01N21/47 ,  G01N21/64

CPC分类号： G01N21/251 ,  G01N21/64 ,  G01N21/8483

摘要： A readhead for illuminating a sample carrier and receiving light from the sample carrier, including a housing for receiving a sample carrier, an array of light sources mounted within the housing in a fixed position relative to the sample carrier, and including first and second light-emitting diodes for emitting substantially monochromatic light of a two different wavelengths, a light guide mounted in the housing between the light-emitting diodes and the sample carrier, and a light detector coupled to receive light from the sample carrier. The readhead also includes a light source for directing excitation light of a predetermined wavelength to the sample carrier, and a light filter positioned between the sample carrier and the light detector and adapted to prevent passage therethrough of the excitation light. The readhead allows both fluorescence spectroscopy and reflectance spectroscopy to be conducted on the sample carrier.

摘要翻译： 一种用于照亮样品载体并从样品载体接收光的读取头，包括用于接收样品载体的壳体，安装在壳体内相对于样品载体固定位置的光源阵列，并且包括第一和第二发光层， 用于发射两个不同波长的基本单色光的发光二极管，安装在发光二极管和样品载体之间的壳体中的光导，以及耦合以接收来自样品载体的光的光检测器。 读取头还包括用于将预定波长的激发光引导到样品载体的光源，以及位于样品载体和光检测器之间并适于防止激发光通过的滤光器。 读数头允许在样品载体上进行荧光光谱和反射光谱。