公开(公告)号：US12116615B2

公开(公告)日：2024-10-15

申请号：US18024502

申请日：2022-05-17

Applicant: DA AN GENE CO., LTD.

Inventor： Xiwen Jiang ,  Wenchuang Qi ,  Runfeng Wu ,  Junxing Jian ,  Chuanfei Mo ,  Yizhu Yin ,  Suwei Lei

IPC: C12Q1/28 ,  B01L3/00

CPC classification number: C12Q1/28 ,  B01L3/5023 ,  B01L2200/16 ,  B01L2300/069 ,  G01N2333/902

Abstract: Disclosed in the present invention are a stabilizer for a color developing agent and application thereof, an application of a composition in preparation of the stabilizer, and a kit. A stabilizer for a color developing agent is provided in the present invention. The stabilizer includes a reducing substance and a weakly acidic buffer, and the weakly acidic buffer has a pH of 3.8-6.2. The reducing substance includes one or more of sodium sulfite, sodium bisulfite, sodium thiosulfate, or 1-mercaptoglycerol. The color developing agent includes one or two of a phenothiazine color developing agent or a triphenylmethane color developing agent. The color developing agent can be stably preserved by using the stabilizer. A method for stably preserving a color developing agent includes dissolving the color developing agent in the stabilizer.

公开(公告)号：US20220145356A1

公开(公告)日：2022-05-12

申请号：US17284476

申请日：2020-10-27

Applicant: DA AN GENE CO., LTD.

Inventor： Yunshao HE ,  Xiwen JIANG ,  Qiao XIA ,  Zhiqiang DONG ,  Lili LIAO ,  Hanrong LI

IPC: C12Q1/6806

Abstract: The present invention discloses a swab sample nucleic acid releaser and use thereof. The sample releaser includes 1-20 mol/L of a metal ion chelating agent, 5-80 mmol/L of a buffer at pH 6-9, 5%-40% (v/v) of a polar organic solvent, 0.2%-10% (v/v) of a surfactant, and 0.1-1 mol/L of a nuclease inhibitor. The sample releaser can integrate three steps of sampling and preservation, inactivation, and nucleic acid extraction into one step, and after sampling, the sample can be directly put into a sampling tube equipped with the sample releaser for storage and transportation; and when in use, the sampling tube is turned upside down and mixed well, and after the sample and the sample releaser are fully mixed, it is let stand for a few minutes at room temperature to release the nucleic acid of the sample.

公开(公告)号：US20230235313A1

公开(公告)日：2023-07-27

申请号：US18019825

申请日：2022-02-11

Applicant: DA AN GENE CO., LTD.

Inventor： Xiwen JIANG ,  Aishan LIU ,  Zhouquan WANG ,  Sangsang ZHENG

IPC: C12N15/10 ,  C12Q1/6806

CPC classification number: C12N15/1006 ,  C12Q1/6806

Abstract: Provided are a separator for separating a nucleic acid amplification system, comprising a polyethylene wax, a solid paraffin wax, and a liquid paraffin wax. Also provided is a method for separating a nucleic acid amplification system, comprising using the separator as a separation layer to separate a nucleic acid amplification system within a same container. The separating layer can be broken by means of applying an external force, thereby mixing the nucleic acid amplification system.

公开(公告)号：US11839877B2

公开(公告)日：2023-12-12

申请号：US17273734

申请日：2020-10-27

Applicant: DA AN GENE CO., LTD.

Inventor： Pengyu Fang ,  Xiwen Jiang

IPC: B01L3/00

CPC classification number: B01L3/5029 ,  B01L2200/16 ,  B01L2300/042 ,  B01L2300/0832

Abstract: The present invention relates to the technical field of sample preservation, and more specifically, to a preservation tube for a sampling swab which includes a tube body. A first cavity for storing a sample releasing agent and a second cavity for placing the sampling swab are provided in the tube body, the first cavity is in communication with the second cavity, and a central axis of the second cavity deviates from a central axis of the tube body. When the present invention is in use, the sampling swab is placed in the second cavity, moved up and down and rotated to elute in the second cavity, and nucleic acid samples of the sampling swab are evenly distributed in the sample releasing agent, and can be used for detection after sucking the sample releasing agent in the first cavity.

公开(公告)号：US20230340561A1

公开(公告)日：2023-10-26

申请号：US18024502

申请日：2022-05-17

Applicant: DA AN GENE CO., LTD.

Inventor： Xiwen JIANG ,  Wenchuang QI ,  Runfeng WU ,  Junxing JIAN ,  Chuanfei MO ,  Yizhu YIN ,  Suwei LEI

IPC: C12Q1/28 ,  B01L3/00

CPC classification number: C12Q1/28 ,  B01L3/5023 ,  B01L2200/16 ,  B01L2300/069 ,  G01N2333/902

Abstract: To the Abstract:
Disclosed in the present invention are a stabilizer for a color developing agent and application thereof, an application of a composition in preparation of the stabilizer, and a kit. A stabilizer for a color developing agent is provided in the present invention. The stabilizer includes a reducing substance and a weakly acidic buffer, and the weakly acidic buffer has a pH of 3.8-6.2. The reducing substance includes one or more of sodium sulfite, sodium bisulfite, sodium thiosulfate, or 1-mercaptoglycerol. The color developing agent includes one or two of a phenothiazine color developing agent or a triphenylmethane color developing agent. The color developing agent can be stably preserved by using the stabilizer. A method for stably preserving a color developing agent includes dissolving the color developing agent in the stabilizer.

公开(公告)号：US11753672B2

公开(公告)日：2023-09-12

申请号：US17284476

申请日：2020-10-27

Applicant: DA AN GENE CO., LTD.

Inventor： Yunshao He ,  Xiwen Jiang ,  Qiao Xia ,  Zhiqiang Dong ,  Lili Liao ,  Hanrong Li

IPC: C12Q1/6806

CPC classification number: C12Q1/6806

Abstract: The present invention discloses a swab sample nucleic acid releaser and use thereof. The sample releaser includes 1-20 mol/L of a metal ion chelating agent, 5-80 mmol/L of a buffer at pH 6-9, 5%-40% (v/v) of a polar organic solvent, 0.2%-10% (v/v) of a surfactant, and 0.1-1 mol/L of a nuclease inhibitor. The sample releaser can integrate three steps of sampling and preservation, inactivation, and nucleic acid extraction into one step, and after sampling, the sample can be directly put into a sampling tube equipped with the sample releaser for storage and transportation; and when in use, the sampling tube is turned upside down and mixed well, and after the sample and the sample releaser are fully mixed, it is let stand for a few minutes at room temperature to release the nucleic acid of the sample.

公开(公告)号：US20220032287A1

公开(公告)日：2022-02-03

申请号：US17273734

申请日：2020-10-27

Applicant: DA AN GENE CO., LTD.

Inventor： Pengyu FANG ,  Xiwen JIANG

IPC: B01L3/00

Abstract: The present invention relates to the technical field of sample preservation, and more specifically, to a preservation tube for a sampling swab which includes a tube body. A first cavity for storing a sample releasing agent and a second cavity for placing the sampling swab are provided in the tube body, the first cavity is in communication with the second cavity, and a central axis of the second cavity deviates from a central axis of the tube body. When the present invention is in use, the sampling swab is placed in the second cavity, moved up and down and rotated to elute in the second cavity, and nucleic acid samples of the sampling swab are evenly distributed in the sample releasing agent, and can be used for detection after sucking the sample releasing agent in the first cavity.