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公开(公告)号：US09845488B2
公开(公告)日：2017-12-19
申请号：US12827210
申请日：2010-06-30
申请人： Christoph Kirsch , Claudia Beyard , Markus Meusel , Klaus Moeller
发明人： Christoph Kirsch , Claudia Beyard , Markus Meusel , Klaus Moeller
CPC分类号： C12Q1/6806 , C12N15/1003
摘要： A method for isolating biomolecules from a fixed biological specimen embedded in paraffin, comprises the steps of bringing the specimen into contact a) with a solvent non-miscible with water and dissolving the paraffin in this solvent such as to form a liquid organic phase, and b) with an aqueous solution of at least one lysis substance and/or with water and at least one lysis substance such as to form an aqueous phase; isolation of the biomolecules from the aqueous phase. The invention further relates to a corresponding kit as well as to the use of such a kit for isolating biomolecules.
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公开(公告)号：US20110003369A1
公开(公告)日：2011-01-06
申请号：US12827210
申请日：2010-06-30
申请人： Christoph KIRSCH , Claudia Beyard , Markus Meusel , Klaus Moeller
发明人： Christoph KIRSCH , Claudia Beyard , Markus Meusel , Klaus Moeller
IPC分类号： C07H1/08
CPC分类号： C12Q1/6806 , C12N15/1003
摘要： A method for isolating biomolecules from a fixed biological specimen embedded in paraffin, comprises the steps of bringing the specimen into contact a) with a solvent non-miscible with water and dissolving the paraffin in this solvent such as to form a liquid organic phase, and b) with an aqueous solution of at least one lysis substance and/or with water and at least one lysis substance such as to form an aqueous phase; isolation of the biomolecules from the aqueous phase. The invention further relates to a corresponding kit as well as to the use of such a kit for isolating biomolecules.
摘要翻译： 从石蜡包埋的固定生物样品中分离生物分子的方法包括以下步骤：使样品与不与水混溶的溶剂接触，并将石蜡溶解在该溶剂中以形成液态有机相， b）与至少一种裂解物质和/或与水和至少一种裂解物质的水溶液形成水相; 从水相中分离生物分子。 本发明还涉及相应的试剂盒以及这种试剂盒用于分离生物分子的用途。
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3.发明授权Chimeric promoters capable of mediating gene expression in plants upon pathogen infection and uses thereof 有权能够在病原体感染中介导植物基因表达的嵌合启动子及其用途公开(公告)号：US08580943B2
公开(公告)日：2013-11-12
申请号：US13225509
申请日：2011-09-05
CPC分类号： C12N15/8216 , C07K14/415 , C07K16/16 , C12N15/8239 , C12N15/8263 , C12N15/8279 , C12N15/8283 , C12Q1/025
摘要： Described are synthetic promoters capable of mediating gene expression in plants upon pathogen infection. Furthermore, recombinant genes and vectors comprising said chimeric promoters as well as host cells transformed with such chimeric promoters, recombinant genes, or vectors are provided. Additionally, diagnostic compositions and kits comprising such chimeric promoters, recombinant genes, vectors or cells are described. Provided are further methods for the identification of compounds being capable of activating or inhibiting genes that are specifically expressed in plants upon pathogen infection employing the above described means. Furthermore, transgenic plant cells, plant tissue, and plants containing the above-described chimeric promoters, recombinant genes, and vectors as well as the use of the aforementioned chimeric promoters, recombinant genes, vectors and/or compounds identified by the method of the invention in plant cell and tissue culture, plant breeding, and/or agriculture are described.
摘要翻译： 描述了能够在病原体感染后介导植物中的基因表达的合成启动子。 此外，提供了包含所述嵌合启动子的重组基因和载体以及用这种嵌合启动子，重组基因或载体转化的宿主细胞。 另外，描述了包含这种嵌合启动子，重组基因，载体或细胞的诊断组合物和试剂盒。 提供了用于鉴定能够激活或抑制在使用上述方法的病原体感染后在植物中特异性表达的基因的化合物的其它方法。 此外，转基因植物细胞，植物组织和含有上述嵌合启动子，重组基因和载体的植物以及通过本发明的方法鉴定的上述嵌合启动子，重组基因，载体和/或化合物的用途 描述了植物细胞和组织培养，植物育种和/或农业。
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公开(公告)号：US20130041145A1
公开(公告)日：2013-02-14
申请号：US13572072
申请日：2012-08-10
申请人： Christoph KIRSCH , Claudia Beyard , Markus Meusel , Thomas Zinn , Carolin Wagner , Klaus Moeller
发明人： Christoph KIRSCH , Claudia Beyard , Markus Meusel , Thomas Zinn , Carolin Wagner , Klaus Moeller
CPC分类号： C12N15/1006 , C12N1/06 , C12N15/1003
摘要： The present invention relates to a method for isolating RNA from a whole blood sample, comprising the steps bringing the sample into contact with an aqueous lysis solution containing at least one lysis substance in a concentration of 1.5 mol/1 to 7 mol/1 and at least one detergent, simultaneously or subsequently adding a proteinase, in particular proteinase K, then incubating the solution for at least partial enzymatic digestion and lysis of the sample so that a lysate is obtained, and isolating the RNA from the lysate.
摘要翻译： 本发明涉及从全血样品中分离RNA的方法，包括使样品与含有至少一种浓度为1.5mol / l至7mol / l的裂解物质的水溶液溶液接触的步骤，以及 至少一种洗涤剂，同时或随后加入蛋白酶，特别是蛋白酶K，然后将溶液温育至少部分酶消化和裂解样品，从而获得裂解物，并从裂解物中分离RNA。
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5.发明申请CHIMERIC PROMOTERS CAPABLE OF MEDIATING GENE EXPRESSION IN PLANTS UPON PATHOGEN INFECTION AND USES THEREOF 有权在病原体感染中介导基因表达的基因启动子及其用途公开(公告)号：US20120117689A1
公开(公告)日：2012-05-10
申请号：US13225509
申请日：2011-09-05
IPC分类号： A01H1/06 , C12N15/82 , C12N15/113
CPC分类号： C12N15/8216 , C07K14/415 , C07K16/16 , C12N15/8239 , C12N15/8263 , C12N15/8279 , C12N15/8283 , C12Q1/025
摘要： Described are synthetic promoters capable of mediating gene expression in plants upon pathogen infection. Furthermore, recombinant genes and vectors comprising said chimeric promoters as well as host cells transformed with such chimeric promoters, recombinant genes, or vectors are provided. Additionally, diagnostic compositions and kits comprising such chimeric promoters, recombinant genes, vectors or cells are described. Provided are further methods for the identification of compounds being capable of activating or inhibiting genes that are specifically expressed in plants upon pathogen infection employing the above described means. Furthermore, transgenic plant cells, plant tissue, and plants containing the above-described chimeric promoters, recombinant genes, and vectors as well as the use of the aforementioned chimeric promoters, recombinant genes, vectors and/or compounds identified by the method of the invention in plant cell and tissue culture, plant breeding, and/or agriculture are described.
摘要翻译： 描述了能够在病原体感染后介导植物中的基因表达的合成启动子。 此外，提供了包含所述嵌合启动子的重组基因和载体以及用这种嵌合启动子，重组基因或载体转化的宿主细胞。 另外，描述了包含这种嵌合启动子，重组基因，载体或细胞的诊断组合物和试剂盒。 提供了用于鉴定能够激活或抑制在使用上述方法的病原体感染后在植物中特异性表达的基因的化合物的其它方法。 此外，转基因植物细胞，植物组织和含有上述嵌合启动子，重组基因和载体的植物以及通过本发明的方法鉴定的上述嵌合启动子，重组基因，载体和/或化合物的用途 描述了植物细胞和组织培养，植物育种和/或农业。
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6.发明授权Chimeric promoters capable of mediating gene expression in plants upon pathogen infection and uses thereof 有权能够在病原体感染中介导植物基因表达的嵌合启动子及其用途公开(公告)号：US08013138B1
公开(公告)日：2011-09-06
申请号：US09831272
申请日：1999-11-12
CPC分类号： C12N15/8282 , A01N63/04 , C07K14/415 , C12N15/8239 , C12N15/8281
摘要： Described are synthetic promoters capable of mediating gene expression in plants upon pathogen infection. Furthermore, recombinant genes and vectors comprising said chimeric promoters as well as host cells transformed with such chimeric promoters, recombinant genes or vectors are provided. Additionally, diagnostic compositions and kits comprising such chimeric promoters, recombinant genes, vectors or cells are described. Provided are further methods for the identification of compounds being capable of activating or inhibiting genes that are specifically expressed in plants upon pathogen infection employing the above described means. Furthermore, transgenic plant cells, plant tissue and plants containing the above-described chimeric promoters, recombinant genes and vectors as well as the use of the aforementioned chimeric promoters, recombinant genes, vectors and/or compounds identified by the method of the invention in plant cell and tissue culture, plant breeding and/or agriculture are described.
摘要翻译： 描述了能够在病原体感染后介导植物中的基因表达的合成启动子。 此外，提供了包含所述嵌合启动子的重组基因和载体以及用这种嵌合启动子，重组基因或载体转化的宿主细胞。 另外，描述了包含这种嵌合启动子，重组基因，载体或细胞的诊断组合物和试剂盒。 提供了用于鉴定能够激活或抑制在使用上述方法的病原体感染后在植物中特异性表达的基因的化合物的其它方法。 此外，含有上述嵌合启动子，重组基因和载体的转基因植物细胞，植物组织和植物以及通过本发明的方法鉴定的上述嵌合启动子，重组基因，载体和/或化合物在植物中的用途 描述细胞和组织培养，植物育种和/或农业。
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