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    IDENTIFYING CANDIDATE CELLS USING IMAGE ANALYSIS WITH OVERLAP THRESHOLDS
    1.
    发明申请
    IDENTIFYING CANDIDATE CELLS USING IMAGE ANALYSIS WITH OVERLAP THRESHOLDS 有权

    公开(公告)号：US20230002835A1

    公开(公告)日：2023-01-05

    申请号：US17940625

    申请日：2022-09-08

    申请人： CellMax Ltd.

    发明人： Huangpin B. Hsieh ,  XiaoMing Wang ,  Jr-Ming Lai ,  Rui Mei ,  Hung-Jen Shao ,  Jen-Chia Wu

    IPC分类号： C12Q1/6886 ,  G01N15/14 ,  G01N33/574 ,  G01N33/50 ,  G01N1/31 ,  G01N33/58

    摘要： A method for identifying candidate target cells within a biological fluid specimen includes a digital image of the biological fluid specimen with the digital image having a plurality of color channels, identifying first connected regions of pixels of a minimum first intensity in a first channel, identifying second connected regions of pixels of a minimum second intensity in a second channel, and determining first connected regions and second connected regions that spatially overlap. For a pair of a first connected region and a second connected region that spatially overlap, whether the second connected region overlaps the first connected region by a threshold amount is determined, and if the second connected region overlaps the first connected region by the threshold amount then the portion of the image corresponding to the overlap is continued to be treated as a candidate for classification.

    IDENTIFYING CANDIDATE CELLS USING IMAGE ANALYSIS WITH INTENSITY LEVELS
    3.
    发明申请
    IDENTIFYING CANDIDATE CELLS USING IMAGE ANALYSIS WITH INTENSITY LEVELS 有权

    公开(公告)号：US20230002834A1

    公开(公告)日：2023-01-05

    申请号：US17940582

    申请日：2022-09-08

    申请人： CellMax Ltd.

    发明人： Huangpin B. Hsieh ,  XiaoMing Wang ,  Jr-Ming Lai ,  Rui Mei ,  Hung-Jen Shao ,  Jen-Chia Wu

    IPC分类号： C12Q1/6886 ,  G01N15/14 ,  G01N33/574 ,  G01N33/50 ,  G01N1/31 ,  G01N33/58

    摘要： Techniques for identifying and enumerating candidate target cells within a biological fluid specimen are described. A digital image of the biological fluid specimen is received, and one or more candidate regions of pixels in the digital image are identified by identifying connected regions of pixels of a minimum intensity having a size between a minimum size and a maximum size and an aspect ratio that meets a threshold. For each candidate region of at least one of the one or more candidate region, whether the portion of the image corresponding to the candidate region includes more than a threshold number of intensity levels is determined. If the portion of the image corresponding to the candidate region includes more than the threshold number of intensity levels the portion of the image is continued to be treated as a candidate for classification.

    METHODS OF CAPTURING A NUCLEIC ACID INCLUDING A TARGET OLIGONUCLEOTIDE SEQUENCE AND USES THEREOF
    4.
    发明申请
    METHODS OF CAPTURING A NUCLEIC ACID INCLUDING A TARGET OLIGONUCLEOTIDE SEQUENCE AND USES THEREOF 审中-公开

    公开(公告)号：US20190316112A1

    公开(公告)日：2019-10-17

    申请号：US15952045

    申请日：2018-04-12

    申请人： CellMax, Ltd.

    发明人： Rui Mei ,  Julian Lucas ,  Pratyush Gupta

    IPC分类号： C12N15/10

    摘要： Provided herein are methods of capturing a nucleic acid comprising a target oligonucleotide sequence from a library of nucleic acid that include: contacting a library of nucleic acids comprising a nucleic acid comprising a target oligonucleotide sequence with a probe comprising a sequence that is complementary to the target oligonucleotide sequence, wherein the contacting is performed in a tetramethylammonium chloride (TMAC)-based buffer at a temperature of about 60° C. to about 70° C., and the contacting results in the hybridization of the target oligonucleotide sequence to the sequence that is complementary to the target oligonucleotide sequence, to thereby generate a hybridization product; and isolating the hybridization product from nucleic acids in the library that do not comprise the target oligonucleotide sequence. Also provided are compositions useful for performing these methods.

    Collection of suspended cells using a transferable membrane
    10.
    发明授权
    Collection of suspended cells using a transferable membrane 有权

    公开(公告)号：US10605708B2

    公开(公告)日：2020-03-31

    申请号：US16124092

    申请日：2018-09-06

    申请人： CellMax, Ltd.

    发明人： Hung-Jen Shao

    IPC分类号： G01N1/00 ,  G01N1/31 ,  B01L3/00 ,  C12M1/26 ,  C12M1/00 ,  B01D63/08 ,  G01N1/30

    摘要： Devices, methods, and kits directed towards collecting and preparing cells using a separable sample collection layer may be configured to collect or treat cells from a liquid sample with mechanisms for easy transfer of the cells prior to analysis or imaging. The separable sample collection layer may comprise a porous membrane that cells may be collected on, and one or more support layers comprising tape with one or more adhesive coatings and release liner. The devices, methods and kits may be configured with support layers comprising cutouts that form vertically or horizontally oriented microchannels for efficiently removing undesirable liquid. Following collection and/or treatment, cells collected onto the porous membrane may be adhered to another surface for further processing or analysis.