公开(公告)号：US10857185B2

公开(公告)日：2020-12-08

申请号：US15741204

申请日：2016-07-01

申请人： Beihao Stem Cell and Regenerative Medicine Research Institutes Co., Ltd. ,  Peking University

发明人： Hongkui Deng ,  Xiang Li ,  Xiaohan Zuo

IPC分类号： C12N5/00 ,  A61K35/30 ,  A61K9/00 ,  C12N5/074 ,  C12N5/0793

摘要： Cocktails of chemical inducers of neuron-like properties (CINP) is provided, which includes cAMP agonists, neurogenic small molecules, glycogen synthase kinase inhibitors, TGFβ receptor inhibitors, and BET family bromodomain inhibitors and optionally, a selective inhibitor of ROCK or p38 MAPK. These cocktails are used in a method of inducing neuron-like properties in partially or completely differentiated non-neuronal cells. The method includes contacting cells of a first type (non-neuronal) with the CINPs for a sufficient period of time to result in reprogramming the cell into cells of a second type having neuron-like characteristics (CiNs). Isolated chemically induced neurons (CiNs) can be used in a number of applications, including but not limited to cell therapy.

公开(公告)号：US20180193386A1

公开(公告)日：2018-07-12

申请号：US15741204

申请日：2016-07-01

申请人： Beihao Stem Cell and Regenerative Medicine Research Institutes Co., Ltd. ,  Peking University

发明人： Hongkui DENG ,  Xiang LI ,  Xiaohan ZUO

IPC分类号： A61K35/30 ,  C12N5/074 ,  C12N5/0793

CPC分类号： A61K35/30 ,  A61K9/0019 ,  C12N5/0607 ,  C12N5/0619 ,  C12N2501/01 ,  C12N2501/115 ,  C12N2501/13 ,  C12N2501/15 ,  C12N2501/60 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2502/086 ,  C12N2503/02 ,  C12N2506/08 ,  C12N2506/1307 ,  C12N2506/1353 ,  C12N2506/1369 ,  C12N2506/1384 ,  C12N2506/1392

摘要： Cocktails of chemical inducers of neuron-like properties (CINP) is provided, which includes cAMP agonists, neurogenic small molecules, glycogen synthase kinase inhibitors, TGFβ receptor inhibitors, and BET family bromodomain inhibitors and optionally, a selective inhibitor of ROCK or p38 MAPK. These cocktails are used in a method of inducing neuron-like properties in partially or completely differentiated non-neuronal cells. The method includes contacting cells of a first type (non-neuronal) with the CINPs for a sufficient period of time to result in reprogramming the cell into cells of a second type having neuron-like characteristics (CiNs). Isolated chemically induced neurons (CiNs) can be used in a number of applications, including but not limited to cell therapy.