Methods for Generation of Reporter Phages and Immobilization of Active Bacteriophages on a Polymer Surface
    2.
    发明申请
    Methods for Generation of Reporter Phages and Immobilization of Active Bacteriophages on a Polymer Surface 审中-公开
    记录噬菌体的生成方法和活性噬菌体在聚合物表面的固定

    公开(公告)号:US20120184012A1

    公开(公告)日:2012-07-19

    申请号:US13353420

    申请日:2012-01-19

    IPC分类号: C12N11/08

    摘要: Novel reporter bacteriophages are provided. Provided are compositions and methods that allow bacteriophages that are used for specific detection or killing of E. coli 0157:H7 to be propagated in nonpathogenic E. coli, thereby eliminating the safety and security risks of propagation in E. coli 0157:H7. Provided are compositions and methods for attaching active bacteriophages to the surface of a polymer in order to kill target bacteria with which the phage comes into contact. Provided are modified bacteriophages immobilized to a surface, which capture E. coli 0157:H7 and cause the captured cells to emit light or fluorescence, allowing detection of the bacteria in a sample.

    摘要翻译: 提供了新的报道噬菌体。 提供了允许用于特异性检测或杀死大肠杆菌0157:H7的噬菌体在非致病性大肠杆菌中繁殖的组合物和方法,从而消除了在大肠杆菌0157:H7中繁殖的安全性和安全性风险。 提供了将活性噬菌体附着到聚合物表面以组合物和方法,以便杀死噬菌体与之接触的目标细菌。 提供了固定在表面上的修饰的噬菌体,其捕获大肠杆菌0157:H7,并使捕获的细胞发出光或荧光,允许检测样品中的细菌。

    Methods for generation of reporter phages and immobilization of active bacteriophages on a polymer surface
    4.
    发明授权
    Methods for generation of reporter phages and immobilization of active bacteriophages on a polymer surface 有权
    产生报道噬菌体的方法和活性噬菌体在聚合物表面上的固定

    公开(公告)号:US08114622B2

    公开(公告)日:2012-02-14

    申请号:US12549500

    申请日:2009-08-28

    IPC分类号: C12Q1/70 C12Q1/66 C12N15/79

    摘要: Novel reporter bacteriophages are provided. Provided are compositions and methods that allow bacteriophages that are used for specific detection or killing of E. coli 0157:H7 to be propagated in nonpathogenic E. coli, thereby eliminating the safety and security risks of propagation in E. coli 0157:H7. Provided are compositions and methods for attaching active bacteriophages to the surface of a polymer in order to kill target bacteria with which the phage comes into contact. Provided are modified bacteriophages immobilized to a surface, which capture E. coli 0157:H7 and cause the captured cells to emit light or fluorescence, allowing detection of the bacteria in a sample.

    摘要翻译: 提供了新的报道噬菌体。 提供了允许用于特异性检测或杀死大肠杆菌0157:H7的噬菌体在非致病性大肠杆菌中繁殖的组合物和方法,从而消除了在大肠杆菌0157:H7中繁殖的安全性和安全性风险。 提供了将活性噬菌体附着到聚合物表面以组合物和方法,以便杀死噬菌体与之接触的目标细菌。 提供了固定在表面上的修饰的噬菌体,其捕获大肠杆菌0157:H7,并使捕获的细胞发出光或荧光,允许检测样品中的细菌。

    Methods for Generation of Reporter Phages and Immobilization of Active Bacteriophages on a Polymer Surface
    5.
    发明申请
    Methods for Generation of Reporter Phages and Immobilization of Active Bacteriophages on a Polymer Surface 有权
    记录噬菌体的生成方法和活性噬菌体在聚合物表面的固定

    公开(公告)号:US20100075301A1

    公开(公告)日:2010-03-25

    申请号:US12549500

    申请日:2009-08-28

    IPC分类号: C12Q1/70 C12N7/00

    摘要: Novel reporter bacteriophages are provided. Provided are compositions and methods that allow bacteriophages that are used for specific detection or killing of E. coli 0157:H7 to be propagated in nonpathogenic E. coli, thereby eliminating the safety and security risks of propagation in E. coli 0157:H7. Provided are compositions and methods for attaching active bacteriophages to the surface of a polymer in order to kill target bacteria with which the phage comes into contact. Provided are modified bacteriophages immobilized to a surface, which capture E. coli 0157:H7 and cause the captured cells to emit light or fluorescence, allowing detection of the bacteria in a sample.

    摘要翻译: 提供了新的报道噬菌体。 提供了允许用于特异性检测或杀死大肠杆菌0157:H7的噬菌体在非致病性大肠杆菌中繁殖的组合物和方法,从而消除了在大肠杆菌0157:H7中繁殖的安全性和安全性风险。 提供了将活性噬菌体附着到聚合物表面以组合物和方法,以便杀死噬菌体与之接触的目标细菌。 提供了固定在表面上的修饰的噬菌体,其捕获大肠杆菌0157:H7,并使捕获的细胞发出光或荧光,允许检测样品中的细菌。