公开(公告)号：US4540282A

公开(公告)日：1985-09-10

申请号：US477634

申请日：1983-03-21

Applicant: Isaac J. Landa ,  Ronald Shideler

Inventor： Isaac J. Landa ,  Ronald Shideler

IPC: G01J3/18 ,  G01J3/28 ,  G01J3/42

CPC classification number: G01J3/42 ,  G01J2003/2866 ,  G01J2003/421 ,  G01J3/1838 ,  G01J3/2889

Abstract: An electro-optical system for rapid, accurate spectral analysis of the reflectivity or transmissivity of samples. A concave, holographic diffraction grating is oscillated at high speed to provide a rapid scanning of monochromatic light through a spectrum of wavelengths. The grating drive system is an electrically driven mechanical oscillator which utilizes the back EMF of the oscillator motor to maintain oscillation at the desired amplitude and frequency. A passive optical shutter mounted to the grating alternately blocks the light entering and exiting the monochrometer as the grating is oscillated. The resultant dark period is utilized by the system to provide a reference offset value and to control cooling of the detectors. A unique phase-locked loop circuit is employed to provide sample commands at precisely determined intervals to correctly correlate the spectral data with the output of the monochrometer. Source and exit optics are employed to optimally shape the light passing through the system. A unique detection head is provided to allow measurement of light transmission or reflectance with only slight modification of the system. An optical fiber is used to divert light from the beam at or near the sample. This light is electronically detected and the electronic detection signal is used to adjust the gain of the sample signal so as to compensate for atmospheric bands, temporal variations, and system response. This provides a true double (dual) beam operation.

Abstract translation: 一种用于对样品的反射率或透射率进行快速，准确的光谱分析的电光系统。 凹入的全息衍射光栅以高速振荡，以提供通过一系列波长的单色光的快速扫描。 光栅驱动系统是电驱动的机械振荡器，其利用振荡器电动机的反电动势将振荡保持在期望的幅度和频率。 安装到光栅的无源光学快门在光栅振荡时交替地阻挡进入和离开单色仪的光。 所产生的暗周期被系统利用以提供参考偏移值并且控制检测器的冷却。 采用独特的锁相环电路以精确确定的间隔提供采样命令，以使光谱数据与单色仪的输出正确相关。 采用光源和出射光学器件来最佳地对穿过系统的光进行成形。 提供独特的检测头以允许仅对系统进行轻微修改来测量光透射或反射。 使用光纤来从样品中或附近的光束转移光线。 该光被电子检测，电子检测信号用于调整采样信号的增益，以补偿大气带，时间变化和系统响应。 这提供了一个真正的双（双）光束操作。

公开(公告)号：US4626684A

公开(公告)日：1986-12-02

申请号：US513307

申请日：1983-07-13

Applicant: Isaac J. Landa

Inventor： Isaac J. Landa

IPC: G01J3/18 ,  G01J3/44 ,  G01N21/64 ,  G01T1/00

CPC classification number: G01N21/6452 ,  G01J3/1833 ,  G01J3/4406 ,  G01N2021/6421 ,  G01N2021/6476 ,  G01N2201/0446

Abstract: An apparatus uses a rapid and efficient method for conducting fluorescence measurements, such as immuno assay, of a plurality of samples of a substance using pulsed or continuous light energy, front-end fluorescent optics, a mechanism which allows for lateral and tranverse scanning of rows and columns of wells, and spectra emission analysis of the spectra from the excited samples.

Abstract translation: 一种装置使用快速和有效的方法进行使用脉冲或连续光能的物质的多个样品的荧光测量，例如免疫测定，前端荧光光学器件，允许横向扫描和横向扫描行的机构 和列的列，以及来自激发样品的光谱的光谱发射分析。