公开(公告)号：US09045760B2

公开(公告)日：2015-06-02

申请号：US13655054

申请日：2012-10-18

申请人： Andrew Reeves ,  Rathin Datta

发明人： Andrew Reeves ,  Rathin Datta

IPC分类号： C12P7/06 ,  C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04 ,  C12N15/74 ,  C12N15/52

CPC分类号： C12N15/74 ,  C12N15/52 ,  C12P7/065 ,  Y02E50/17

摘要： Gene sequences of key acetogenic clostridial species were sequenced and isolated. Genes of interest were identified, and functionality was established. Key genes of interest for metabolic catalyzing activity in clostridial species include a three-gene operon coding for CODH activity, a two-gene operon coding for PTA-ACK, and a novel acetyl coenzyme A reductase. The promoter regions of the two operons and the acetyl coA reductase are manipulated to increase ethanol production.

摘要翻译： 对重要的乙型梭菌种的基因序列进行测序和分离。 确定了感兴趣的基因，建立了功能。 梭菌中代谢催化活性的关键基因包括编码CODH活性的三基因操纵子，编码PTA-ACK的双基因操纵子和新型乙酰辅酶A还原酶。 操纵两个操纵子的启动子区域和乙酰辅酶A还原酶以增加乙醇生成。

公开(公告)号：US08980597B2

公开(公告)日：2015-03-17

申请号：US13243347

申请日：2011-09-23

申请人： Robert Hickey ,  Steven L. Donnellan

发明人： Robert Hickey ,  Steven L. Donnellan

IPC分类号： C12P7/06 ,  C12P1/04 ,  C12P7/04 ,  C12P7/24 ,  C12P7/40

CPC分类号： C12P7/04 ,  C12P7/06 ,  C12P7/16 ,  C12P7/24 ,  C12P7/40 ,  C12P7/54 ,  Y02E50/10 ,  Y02E50/17

摘要： Processes are disclosed for the low energy, anaerobic bioconversion of hydrogen and carbon monoxide in a gaseous substrate stream to oxygenated organic compounds such as ethanol by contact with microorganisms in a fermentation system with high conversion efficiency of both hydrogen and carbon monoxide. The processes of this invention use a pre-reactor and a deep, tank reactor in gaseous substrate flow sequence to obtain high conversion of gas substrate without undue risk of carbon monoxide inhibition.

摘要翻译： 公开了用于在具有高转化效率的氢气和一氧化碳的发酵系统中通过与微生物接触的气态底物物流中的氢和一氧化碳的低能，厌氧生物转化为含氧有机化合物如乙醇的方法。 本发明的方法使用气态底物流程序列中的预反应器和深槽反应器，以获得气体底物的高转化率，而不会产生一氧化碳抑制的过度风险。

公开(公告)号：US20150028260A1

公开(公告)日：2015-01-29

申请号：US14512082

申请日：2014-10-10

申请人： Richard E. Tobey ,  Jianxin Du ,  Ralph Corley

发明人： Richard E. Tobey ,  Jianxin Du ,  Ralph Corley

IPC分类号： C01B3/02

CPC分类号： C01B3/02 ,  B01D2257/408 ,  C01B3/36 ,  C01B2203/0255 ,  C01B2203/0415 ,  C01B2203/043 ,  C01B2203/0475 ,  C01B2203/048 ,  C01B2203/0485 ,  C01B2203/0495 ,  C01B2203/0877 ,  C01B2203/0894 ,  C01B2203/141 ,  C01B2203/142 ,  C01B2203/146 ,  C01B2203/1671 ,  C01B2203/1676 ,  C01B2203/169 ,  C12M21/12 ,  C12M45/06 ,  C12P7/065 ,  C12P7/08 ,  C12P7/16 ,  C12P7/52 ,  C12P7/54 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/17

摘要： Processes are disclosed for the conversion of biomass to oxygenated organic compound using a simplified syngas cleanup operation that is cost effective and protects the fermentation operation. The processes of this invention treat the crude syngas from the gasifier by non-catalytic partial oxidation. The partial oxidation reduces the hydrocarbon content of the syngas such as methane, ethylene and acetylene to provide advantageous gas feeds for anaerobic fermentations to produce oxygenated organic compounds such as ethanol, propanol and butanol. Additionally, the partial oxidation facilitates any additional cleanup of the syngas as may be required for the anaerobic fermentation. Producer gases and partial oxidation processes are also disclosed.

摘要翻译： 公开了使用简化的合成气净化操作将生物质转化为含氧有机化合物的方法，其成本有效并且保护发酵操作。 本发明的方法通过非催化部分氧化处理来自气化器的粗合成气。 部分氧化降低了甲烷，乙烯和乙炔等合成气的烃含量，为厌氧发酵提供了有利的气体进料，以产生含氧有机化合物如乙醇，丙醇和丁醇。 另外，部分氧化促进了对厌氧发酵所需要的合成气的任何额外的清除。 还公开了生产气体和部分氧化方法。

公开(公告)号：US08828692B2

公开(公告)日：2014-09-09

申请号：US13101535

申请日：2011-05-05

申请人： Robert Hickey ,  Rathin Datta ,  Shih-Perng Tsai ,  Rahul Basu

发明人： Robert Hickey ,  Rathin Datta ,  Shih-Perng Tsai ,  Rahul Basu

IPC分类号： C12P7/54 ,  C12M1/00 ,  C12M1/12 ,  C12P7/04 ,  C12M1/34

CPC分类号： C12P7/065 ,  C12M21/12 ,  C12M23/58 ,  C12M25/10 ,  C12M29/16 ,  C12M41/28 ,  C12P7/04 ,  Y02E50/17

摘要： Ethanol and other liquid products are produced by contacting syngas components such as CO or a mixture of CO2 and H2 with a surface of a membrane under anaerobic conditions and transferring these components into contact with a biofilm on the opposite side of the membrane. These steps provide a stable system for producing liquid products such as ethanol, butanol and other chemicals. The gas fed on the membrane's gas contact side transports through the membrane to form a biofilm of anaerobic microorganisms that converted the syngas to desired liquid products. The system can sustain production with a variety of microorganisms and membrane configurations.

摘要翻译： 乙醇和其它液体产物是通过将合成气组分如CO或CO 2和H 2的混合物与膜的表面在厌氧条件下接触而生产的，并将这些组分与膜的相反侧的生物膜接触。 这些步骤为生产液体产品如乙醇，丁醇和其他化学品提供了稳定的系统。 供给在膜的气体接触侧的气体输送通过膜以形成将合成气转化为所需液体产物的厌氧微生物生物膜。 该系统可以通过各种微生物和膜结构来维持生产。

公开(公告)号：US08486359B2

公开(公告)日：2013-07-16

申请号：US13190355

申请日：2011-07-25

申请人： Robert Hickey

发明人： Robert Hickey

IPC分类号： C01C3/00

CPC分类号： C01C1/26 ,  C10G2300/1011 ,  C10K1/08 ,  Y02P30/20

摘要： The processes are utilized to recover ammonium from waste water using CO2 acidified absorption water. The process is particularly suited for utilization of cellular matter and a CO2 rich tail gas from a syngas fermentation process and derives significant benefit from the recovery of ammonium bicarbonate and ammonium carbonate. Ammonia and ammonium are recovered from the treatment of the syngas as an ammonium rich solution, at least a portion of which is recycled to the fermentation zone to aid in the production of liquid products. A carbon dioxide rich gas produced by fermentation is used to capture the ammonia and ammonium, forming the ammonium rich solution.

摘要翻译： 该方法用于使用CO2酸化吸收水从废水中回收铵。 该方法特别适用于从合成气发酵过程中利用细胞物质和富含CO 2的尾气，并从碳酸氢铵和碳酸铵的回收中获得显着的益处。 从作为富铵溶液的合成气的处理中回收氨和铵，其中至少一部分被再循环到发酵区以帮助生产液体产物。 通过发酵产生的富含二氧化碳的气体用于捕获氨和铵，形成富铵溶液。

公开(公告)号：US20130078689A1

公开(公告)日：2013-03-28

申请号：US13243426

申请日：2011-09-23

申请人： Robert Hickey

发明人： Robert Hickey

IPC分类号： C12P7/00 ,  C12P7/04 ,  C12M1/36 ,  C12P7/52 ,  C12P7/16 ,  C12M1/107 ,  C12P7/06 ,  C12P7/54

CPC分类号： C12P7/54 ,  C12M21/12 ,  C12M27/02 ,  C12M29/06 ,  C12P7/00 ,  C12P7/04 ,  C12P7/06 ,  C12P7/065 ,  C12P7/16 ,  C12P7/52 ,  Y02E50/10 ,  Y02E50/17 ,  Y02P20/582

摘要： Processes and apparatus are disclosed for the low energy, anaerobic bioconversion of hydrogen and carbon monoxide in a gaseous substrate stream to oxygenated organic compounds such as ethanol by contact with microorganisms in a deep, stirred tank fermentation system with high conversion efficiency of both hydrogen and carbon monoxide. Gas feed to the reactor is injected using a motive liquid to form a stable dispersion of microbubbles thereby reducing energy costs, and a portion of the off-gases from the reactor are recycled to (i) achieve a conversion of the total moles of carbon monoxide and hydrogen in the gas substrate to oxygenated organic compound of at least about 80 percent and (ii) attenuate the risk of carbon monoxide inhibition of the microorganism used for the bioconversion.

摘要翻译： 公开的方法和装置用于在气态底物物流中的氢和一氧化碳的低能，厌氧生物转化为氧化的有机化合物如乙醇，通过与氢气和碳的高转化效率的深层搅拌罐发酵系统中的微生物接触 一氧化碳 使用运动液体注入反应器的气体进料以形成稳定的微泡分散体，从而降低能量成本，并将一部分来自反应器的废气再循环至（i）实现一氧化碳总摩尔数的转化 和气体底物中的氢气至少约80％的氧化有机化合物，和（ii）减弱用于生物转化的微生物的一氧化碳抑制的风险。

公开(公告)号：US08211692B2

公开(公告)日：2012-07-03

申请号：US12258193

申请日：2008-10-24

申请人： Rathin Datta ,  Shih-Perng Tsai ,  Jian Xu ,  Seong-Hoon Yoon

发明人： Rathin Datta ,  Shih-Perng Tsai ,  Jian Xu ,  Seong-Hoon Yoon

IPC分类号： C02F3/34 ,  A62D3/00 ,  A62D3/02 ,  C12P7/06

CPC分类号： C12P7/06 ,  B01D53/84 ,  B01D63/02 ,  B01D2311/2688 ,  B01D2317/04 ,  C12M29/16 ,  C12P7/04 ,  C12P7/16 ,  C12P7/52 ,  C12P7/54 ,  Y02A50/2358 ,  Y02E50/10 ,  Y02E50/17 ,  Y02P20/59

摘要： The bioconversion of gas feedstreams to liquid products by direct contact with a layer of microorganism obtains enhanced productivity through the regular cycling of liquid across a substrate that supports a biolayer of microorganisms while separating the gas and liquid phases. Such processes produce liquid products such as ethanol, butanol and other chemicals from syngas components by contacting CO or a mixture of CO2 and H2 with a highly porous side of an asymmetric membrane under anaerobic conditions and transferring these components into contact with microorganisms contained within bio-pores of the membrane. A periodic laving of liquid from the liquid contact side to and away from the microorganisms can increase nutrient flow to the microorganisms while enhancing the recovery of liquid products. The process effects laving by temporarily raising the liquid phase pressure relative to the gas phase pressure to periodically surge liquid toward the microorganisms and then restoring the original pressure differential to urge liquid away from the microorgaisms.

摘要翻译： 通过与一层微生物的直接接触将气体进料流生物转化成液体产物，通过在分离气相和液相的基础上常规循环液体，从而提高生产率。 这样的方法通过在无氧条件下将CO或CO 2和H 2的混合物与不对称膜的高度多孔侧接触，并将这些成分与生物相容性中所含的微生物接触，从而从合成气组分产生液体产物如乙醇，丁醇和其它化学物质， 膜的孔。 从液体接触侧到微生物的周期性的液体可以增加对微生物的营养物流，同时增强液体产物的回收。 该过程通过临时提高相对于气相压力的液相压力而使微生物周期性地向上移动液体，然后恢复原始压力差以迫使液体远离微观结构。

公开(公告)号：US08143037B2

公开(公告)日：2012-03-27

申请号：US12727320

申请日：2010-03-19

申请人： James A. Zahn ,  Jyotisna Saxena

发明人： James A. Zahn ,  Jyotisna Saxena

IPC分类号： C12P7/06 ,  C12N1/20

CPC分类号： C12P7/08 ,  C12P7/065 ,  C12R1/145 ,  Y02E50/17

摘要： An isolated clostridia bacterial species (Clostridium coskatii ATCC No. PTA-10522, “PS02”) is provided. Under anaerobic conditions C. coskatii can convert CO and/or H2 and/or CO2 to ethanol or acetate. Thus, this bacterium is capable of transforming waste gases (e.g. syngas and refinery wastes) into useful products.

摘要翻译： 提供了分离的梭菌菌种（Clostridium coskatii ATCC No.PTA-10522，“PS02”）。 在厌氧条件下，coskatii可以将CO和/或H 2和/或CO 2转化为乙醇或乙酸酯。 因此，该细菌能够将废气（例如合成气和炼油厂废物）转化成有用的产物。

公开(公告)号：US20160130610A1

公开(公告)日：2016-05-12

申请号：US14536413

申请日：2014-11-07

申请人： Shih-Perng Tsai ,  Robert Hickey ,  Jianxin Du ,  Jian Xu ,  Joshua Schumacher

发明人： Shih-Perng Tsai ,  Robert Hickey ,  Jianxin Du ,  Jian Xu ,  Joshua Schumacher

IPC分类号： C12P7/06

CPC分类号： C12P7/065 ,  C12P7/16 ,  C12P7/54 ,  Y02E50/10 ,  Y02E50/17 ,  Y02P20/582

摘要： Processes for the bioconversion of syngas to oxygenated organic compound are disclosed that reliably, cost-effectively and efficiently supply sulfur nutrient to microorganisms contained in acidic, aqueous fermentation menstrua. In the processes of this invention, basic, aqueous solution used to maintain the pH of the aqueous fermentation menstruum is used to remove hydrogen sulfide from the off-gas from the fermentation menstruum for recycle to the fermentation menstruum.

摘要翻译： 公开了将合成气生物转化为含氧有机化合物的方法，其可靠地，经济有效且有效地向酸性水性发酵人体中所含微生物提供硫营养物。 在本发明的方法中，使用用于保持含水发酵液的pH值的碱性水溶液从发酵罐的废气中除去硫化氢，以再循环至发酵液。

公开(公告)号：US09034617B2

公开(公告)日：2015-05-19

申请号：US13828542

申请日：2013-03-14

申请人： COSKATA, INC.

发明人： Robert Hickey ,  Jianxin Du ,  Andrew Reeves ,  Richard E. Tobey

IPC分类号： C12P7/16 ,  C12P7/06 ,  C12P7/04

CPC分类号： C12P7/16 ,  C12N1/20 ,  C12N1/38 ,  C12P7/04 ,  C12P7/06 ,  Y02E50/10 ,  Y02E50/17

摘要： Processes are disclosed for the anaerobic bioconversion of syngas to oxygenated organic compound that use an in situ method for protecting the microorganisms from hydrogen cyanide contained in the syngas that passes to the fermentation broth. The fermentation broth is maintained at a pH of between about 4 and 6, and dissolved metal cation of one or more of iron, cobalt, nickel and zinc is provided to the fermentation broth in an amount sufficient to form, under the conditions of the fermentation broth, a substantially insoluble metal complex with the metal cation and cyanide anion. The rate of formation of the insoluble complex is sufficiently high that that the amount of cyanide that is taken up by microorganisms does not result in an undue adverse effect on the population of microorganisms.

摘要翻译： 公开了将合成气厌氧生物转化为含氧有机化合物的方法，其使用原位方法保护微生物免受通过发酵液的合成气中所含的氰化氢。 发酵液的pH值保持在4〜6左右之间，并在发酵液中提供一种或多种铁，钴，镍，锌溶解的金属阳离子，其量足以在发酵条件下形成 肉汤，与金属阳离子和氰化物阴离子基本不溶的金属络合物。 不溶性络合物的形成速率足够高，使得微生物吸收的氰化物的量不会对微生物群体产生不适当的不利影响。