知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

913 条记录 (耗时 0.037 秒)

    NANOFLUIDIC DEVICES FOR THE RAPID MAPPING OF WHOLE GENOMES AND RELATED SYSTEMS AND METHODS OF ANALYSIS
    71.
    发明申请
    NANOFLUIDIC DEVICES FOR THE RAPID MAPPING OF WHOLE GENOMES AND RELATED SYSTEMS AND METHODS OF ANALYSIS 有权
    用于快速映射全基因组的相关系统和相关系统的分析方法及分析方法

    公开(公告)号：US20160024569A1

    公开(公告)日：2016-01-28

    申请号：US14771989

    申请日：2014-03-11

    申请人： THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL

    发明人： John Michael Ramsey ,  Laurent Menard

    IPC分类号： C12Q1/68 ,  G01N27/447 ,  G01N33/487

    CPC分类号： G01N27/44791 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2300/0896 ,  B01L2400/0418 ,  B01L2400/0421 ,  C12Q1/683 ,  C12Q1/6869 ,  G01N33/48721 ,  C12Q2561/113 ,  C12Q2565/631

    摘要： Devices and methods generate an ordered restriction map of genomic DNA extracted from whole cells, nuclei, whole chromosomes, or other sources of long DNA molecules. The devices have a fluidic microchannel that merges into a reaction nanochannel that merges into a detection nanochannel at an interface where the nanochannel diameter decreases in size by between 50% to 99%. Intact molecules of DNA are transported to the reaction nanochannel and then fragmented in the reaction nanochannel using restriction endonuclease enzymes. The reaction nanochannel is sized and configured so that the fragments stay in an original order until they are injected into the detection nanochannel. Signal at one or more locations along the detection nanochannel is detected to map fragments in the order they occur along a long DNA molecule.

    摘要翻译： 设备和方法产生从全细胞，核，全染色体或其他长DNA分子来源提取的基因组DNA的有序限制图谱。 这些装置具有流体微通道，其融合到纳米通道直径在大小减小50％至99％之间的界面处合并到检测纳米通道中的反应纳米通道。 将完整的DNA分子转运到反应纳米通道，然后使用限制性内切核酸酶在反应纳米通道中分裂。 反应纳米通道的尺寸和构造使得片段保持原始顺序，直到它们被注入到检测纳米通道中。 检测沿着检测纳米通道的一个或多个位置处的信号以沿着长DNA分子发生的顺序映射片段。

    NONDESTRUCTIVE COLLECTION OF EVIDENCE
    75.
    发明申请
    NONDESTRUCTIVE COLLECTION OF EVIDENCE 审中-公开
    非结构性证据收集

    公开(公告)号：US20150225783A1

    公开(公告)日：2015-08-13

    申请号：US14618164

    申请日：2015-02-10

    申请人： LOCKHEED MARTIN CORPORATION

    发明人： John Cleon Mears ,  Jeffrey A. Stuart

    IPC分类号： C12Q1/68 ,  H04N5/225

    CPC分类号： C12Q1/6869 ,  A61B10/02 ,  A61B2010/0216 ,  C12Q1/68 ,  G01N21/55 ,  G06K2009/00946 ,  H04N5/2256 ,  C12Q2563/185 ,  C12Q2565/607 ,  C12Q2565/619 ,  C12Q2565/631

    摘要： A system and method of identifying a print includes an image-capturing and lighting optical system configured to maximize specular reflection of light reflected from a print and to minimize diffused reflection of light reflected from a background surface of the print via adjustment of at least one of a frequency and a reflection angle of the light emitted upon a sample of the print. The system and method also include an IC having one or more FETs with a nanostructure configured to detect a plurality of analytes from the print. The system and method also include a nucleic acid analyzer configured to process the print and to determine a DNA content of the print. There is no contact made with the print, while being subjected to processing by the image-capturing and lighting optical system and the IC.

    摘要翻译： 识别打印件的系统和方法包括图像拍摄和照明光学系统，其被配置为最大化从打印反射的光的镜面反射，并且通过调节以下各项中的至少一个来最小化从印刷物的背景表面反射的光的漫反射 发射在印刷品样品上的光的频率和反射角。 该系统和方法还包括具有一个或多个具有纳米结构的FET的IC，该纳米结构被配置为从印刷体检测多个分析物。 该系统和方法还包括配置用于处理印刷品并确定印刷品的DNA含量的核酸分析仪。 在通过图像拍摄和照明光学系统和IC进行处理的同时，没有与打印件进行接触。

    SSB METHOD
    76.
    发明申请
    SSB METHOD 审中-公开
    SSB方法

    公开(公告)号：US20150197796A1

    公开(公告)日：2015-07-16

    申请号：US14415459

    申请日：2013-07-18

    申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED

    发明人： James White ,  Ruth Moysey ,  Mihaela Misca

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  C12Q2522/101 ,  C12Q2523/31 ,  C12Q2565/631

    摘要： The invention relates to a method of characterising a target polynucleotide using a single-stranded binding protein (SSB). The SSB is either an SSB comprising a carboxy-terminal (C-terminal) region which does not have a net negative charge or a modified SSB comprising one or more modifications in its C-terminal region which decreases the net negative charge of the C-terminal region.

    摘要翻译： 本发明涉及使用单链结合蛋白（SSB）表征靶多核苷酸的方法。 SSB是包含不具有净负电荷的羧基末端（C末端）区域的SSB或包含其C末端区域中的一个或多个修饰的修饰SSB，其降低C-末端区域的净负电荷， 终端区域。

    COMPOSITIONS AND METHODS FOR IMPROVING NANOPORE SEQUENCING
    77.
    发明申请
    COMPOSITIONS AND METHODS FOR IMPROVING NANOPORE SEQUENCING 审中-公开
    用于改进纳米级序列的组合物和方法

    公开(公告)号：US20150191782A1

    公开(公告)日：2015-07-09

    申请号：US14419217

    申请日：2013-08-02

    申请人： University of Washington through its Center for Commercialization

    发明人： Jens H. Gundlach ,  Andrew Laszlo

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  C12Q2523/31 ,  C12Q2565/631

    摘要： The present disclosure provides methods and reagents for improving nanopore-based analyses of polymers. Specifically, the disclosure provides a method of analyzing a polymer that includes a polymer analyte that contains an end domain that has at least one charged moiety. The disclosure also provides a method of increasing the interaction rate between a polymer analyte and a nanopore, wherein the polymer analyte contains an end domain that has at least one charged moiety. The disclosure also provide compositions for use with the described methods, including adapter compositions that contain charged moieties, such as phosphate or sulfate groups, and that are configured to being linked to an polymer analyte domain.

    摘要翻译： 本公开提供了用于改进聚合物的基于纳米孔的分析的方法和试剂。 具体地，本公开提供了一种分析包含聚合物分析物的聚合物的方法，所述聚合物分析物含有具有至少一个带电部分的末端结构域。 本公开还提供了增加聚合物分析物和纳米孔之间的相互作用速率的方法，其中聚合物分析物含有具有至少一个带电部分的末端结构域。 本公开还提供了与所述方法一起使用的组合物，包括含有带电部分的接合体组合物，例如磷酸盐或硫酸盐基团，并且被配置为连接到聚合物分析物结构域。

    Method of analysis of genetic markers
    78.
    发明授权
    Method of analysis of genetic markers 有权
    遗传标记分析方法

    公开(公告)号：US09068221B2

    公开(公告)日：2015-06-30

    申请号：US13370177

    申请日：2012-02-09

    申请人： Barry Merriman ,  Paul Mola

    发明人： Barry Merriman ,  Paul Mola

    IPC分类号： C12Q1/68

    CPC分类号： G01N33/48721 ,  C12Q1/6816 ,  C12Q1/6827 ,  G01N27/26 ,  C12Q2537/16 ,  C12Q2565/631

    摘要： A method of analyzing genetic markers includes binding a set of probes to a segment of single stranded nucleic acids. The segment of single stranded nucleic acids includes a repeat region formed of at least two of a repeat unit. The repeat unit can include at least two nucleic acids. The set of probes includes a first probe complementary to the repeat unit. The method can further include directing the segment through a nanopore device and measuring a signal through the nanopore device. The signal can be indicative of the number of repeat units.

    摘要翻译： 分析遗传标记的方法包括将一组探针结合到单链核酸的片段。 单链核酸的片段包括由重复单元中的至少两个形成的重复区域。 重复单元可以包括至少两个核酸。 该组探针包括与重复单元互补的第一探针。 该方法可以进一步包括将片段引导通过纳米孔装置并通过纳米孔装置测量信号。 该信号可以指示重复单元的数量。