知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

976 条记录 (耗时 0.033 秒)

    COMPOSITIONS AND METHODS FOR ROLLING CIRCLE AMPLIFICATION
    51.
    发明申请
    COMPOSITIONS AND METHODS FOR ROLLING CIRCLE AMPLIFICATION 有权

    公开(公告)号：US20230044650A1

    公开(公告)日：2023-02-09

    申请号：US17816985

    申请日：2022-08-02

    申请人： 10x Genomics, Inc.

    发明人： Rhyan Blaine DOCKTER

    IPC分类号： C12Q1/6853

    摘要： The present disclosure in some aspects relates to methods and compositions for accurately detecting and quantifying multiple analytes present in a biological sample. In some aspects, the methods and compositions provided herein address one or more issues associated with the stability and/or size of nucleic acid structures such as rolling circle amplification products in the biological sample.

    Method and system for sampling material from cells
    53.
    发明授权
    Method and system for sampling material from cells 有权

    公开(公告)号：US11542541B2

    公开(公告)日：2023-01-03

    申请号：US17105333

    申请日：2020-11-25

    申请人： Bio-Rad Laboratories, Inc.

    发明人： Arkadiusz Bibillo ,  Pranav Patel ,  Christopher Reggiardo ,  Jonathan Petersen

    IPC分类号： C12Q1/68 ,  C12Q1/6806 ,  C12N15/10 ,  C12Q1/6853 ,  C12Q1/6874

    摘要： Methods, systems, and devices for sampling/isolating material from cells. An exemplary system may comprise a chip including an electrode array of sampling electrodes arranged along a surface of the chip. A cell-receiving area may be located adjacent the surface of the chip. The system also may comprise a tag array of tags supported by the chip and aligned with the electrode array. Each tag of the tag array may include an identifier that is unique to the tag within the tag array. Each tag may be configured to bind nucleic acids, or a capturing agent distinct from the tag may be aligned with each sampling electrode of the electrode array to capture a protein or other analyte of interest. The system further may comprise a control circuit configured to apply an individually controllable voltage to each sampling electrode of the electrode array and measure an electrical property of the sampling electrode.

    PRIMERS WITH SELF-COMPLEMENTARY SEQUENCES FOR MULTIPLE DISPLACEMENT AMPLIFICATION
    54.
    发明申请
    PRIMERS WITH SELF-COMPLEMENTARY SEQUENCES FOR MULTIPLE DISPLACEMENT AMPLIFICATION 有权

    公开(公告)号：US20220364165A1

    公开(公告)日：2022-11-17

    申请号：US17849078

    申请日：2022-06-24

    申请人： QIAGEN SCIENCES, LLC

    发明人： Yexun WANG ,  Quan PENG

    IPC分类号： C12Q1/6853

    摘要： The present disclosure provides primers, primer sets, kits and methods for multiple displacement amplification, especially in combination with nucleic acid sequencing. The primers comprise self-complementary sequences at their 5′ termini and random or semi-random sequences at their 3′ termini. Use of such primers facilitates handling of multiple samples, increases sequence coverage uniformity, and improves sequencing error corrections.

    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR
    57.
    发明申请
    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR 有权

    公开(公告)号：US20220315917A1

    公开(公告)日：2022-10-06

    申请号：US17302674

    申请日：2021-05-10

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Mark ANDERSEN ,  Daniel MAZUR ,  Sihong CHEN ,  Guobin LUO ,  Xinzhan PENG

    IPC分类号： C12N15/10 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6855

    摘要： Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.