公开(公告)号：US06579718B1

公开(公告)日：2003-06-17

申请号：US09632927

申请日：2000-08-04

Applicant: Stephen T. Yue ,  Thomas H. Steinberg ,  Wayne F. Patton ,  Ching-Ying Cheung ,  Richard P. Haugland

Inventor： Stephen T. Yue ,  Thomas H. Steinberg ,  Wayne F. Patton ,  Ching-Ying Cheung ,  Richard P. Haugland

IPC: G01N3348

CPC classification number: C07D401/06 ,  C07D209/08 ,  C07D209/86 ,  C07D213/20 ,  C07D215/10 ,  C07D277/64 ,  C07D417/06 ,  C09B23/105 ,  G01N33/6809 ,  Y10T436/25125 ,  Y10T436/25375

Abstract: The present invention describes a variety of substituted and unsubstituted carbazolylvinyl dyes and their use for detecting and quantifying poly(amino acids), including peptides, polypeptides and proteins. The labeled proteins or peptides are highly colored, but are also detected by their strong fluorescence enhancement. Poly(amino acids) are detected in solution, in electrophoretic gels, and on solid supports, including blots and dipsticks. The present method of staining is highly sensitive, extremely facile, and relatively non-selective and can be accomplished without the use of organic solvent additives.

Abstract translation: 本发明描述了各种取代和未取代的咔唑基乙烯基染料及其用于检测和定量聚（氨基酸）的用途，包括肽，多肽和蛋白质。 标记的蛋白质或肽是高度着色的，但也通过其强荧光增强来检测。 聚（氨基酸）在溶液中，电泳凝胶和固体支持物上检测，包括印迹和浸渍。 本发明的染色方法是高度敏感的，非常容易的和相对非选择性的，并且可以在不使用有机溶剂添加剂的情况下完成。

公开(公告)号：US20120288855A1

公开(公告)日：2012-11-15

申请号：US13559300

申请日：2012-07-26

Applicant: Jolene A. Bradford ,  Ching-Ying Cheung ,  Shih-Jung Huang ,  Patrick R. Pinson ,  Stephen T. Yue

Inventor： Jolene A. Bradford ,  Ching-Ying Cheung ,  Shih-Jung Huang ,  Patrick R. Pinson ,  Stephen T. Yue

IPC: G01N21/76 ,  C07D417/14 ,  C07D413/06 ,  C07D417/06

CPC classification number: G01N33/582 ,  C07D215/10 ,  C07D215/227 ,  C07D277/72 ,  C07D277/74 ,  C07D417/04 ,  C07D417/06 ,  C07D417/14 ,  C12Q1/6876 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2201/06113 ,  Y10T436/143333

Abstract: Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.

Abstract translation: 公开了对RNA和单链DNA具有对双链DNA的高选择性的化合物。 化学化合物是在照射时变成荧光并与双链DNA相互作用的污渍，但在不存在双链DNA的情况下表现出减少的或没有荧光。 该化合物可用于各种生物学应用中，以定性或定量测定DNA，即使在RNA的存在下。

公开(公告)号：US07598390B2

公开(公告)日：2009-10-06

申请号：US11432814

申请日：2006-05-11

Applicant: Jolene A. Bradford ,  Ching-Ying Cheung ,  Shih-Jung Huang ,  Patrick R. Pinson ,  Stephen T. Yue

Inventor： Jolene A. Bradford ,  Ching-Ying Cheung ,  Shih-Jung Huang ,  Patrick R. Pinson ,  Stephen T. Yue

IPC: C07D417/00 ,  C07D215/00 ,  C07D251/38

CPC classification number: G01N33/582 ,  C07D215/10 ,  C07D215/227 ,  C07D277/72 ,  C07D277/74 ,  C07D417/04 ,  C07D417/06 ,  C07D417/14 ,  C12Q1/6876 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2201/06113 ,  Y10T436/143333

Abstract: Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.

Abstract translation: 公开了对RNA和单链DNA具有对双链DNA的高选择性的化合物。 化学化合物是在照射时变成荧光并与双链DNA相互作用的污渍，但在不存在双链DNA的情况下表现出减少的或没有荧光。 该化合物可用于各种生物学应用中，以定性或定量测定DNA，即使在RNA的存在下。